LOCUS       sequence-17379-e         231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, 
            Simonitch TA, Hack FM, Lawrence HJ
  TITLE     Lineage-restricted expression of homeobox-containing genes in human 
            hematopoietic cell lines.
  JOURNAL   Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40.
  PUBMED    2573064
REFERENCE   2  (bases 1 to 231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1989-11"; volume: "86(21)"; pages: "8536-40"
COMMENT     Sequence Label: HOXB4 (human) HIS-tag pET new RBS
FEATURES             Location/Qualifiers
     source          1..231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..65
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             69..101
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
       61 gcagccatat ggctagcatg actggtggac agcaaatggg tcgcggatcc gaattcgcca
      121 ccatggctat gagttctttt ttgatcagct caaactatgt cgaccccaag ttccctccat
      181 gcgaggaata ttcacagagc gattacctac ccagcgacca ctcgcccggg a
//
