LOCUS       sequence-173790-        3519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the square shown in figure 3F of associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_square
FEATURES             Location/Qualifiers
     source          1..3519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1042..1064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1053..1069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1077..1083,1084..1101,1102..1107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1122..1143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1260..1277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1431..2019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1511..1530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2321..3115
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2375..2394)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2985..3004
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3280..3298
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3336..3358)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3458..3477
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgtcagtta cgtcgagcag aatactgtgg aaatccaaga gcgctgatta cattcgcagt
       61 tagtaagatt ttatcactgt aaagattcct atactcaaga attttaacct cgctggacat
      121 agatagtgag ggtctctaat gtcccaaact ctatccagga tggaagaata caatcccaca
      181 tccggtccgt gttagcgatg ctccacggat cgccaatgag ccacttgtaa agccggatcc
      241 tcagtcagta aattggactg gactatccga gcgttgtcca tgcctttatg cttggaacga
      301 cagcgatcat ctgaatacaa gtcttactcg tgataatttg tgcgatctgc tctgagatcg
      361 agatgatccg ctgatgagca gccctcctta gcatagctag ccctaagtga gcttcaggcc
      421 atgagacagc caaggggtca gaggtattag aggtgcgctg tacagggggt agtgttcgac
      481 gaaacgttac gcctcgtatc gacttcgaga tctatgaatt atgattgtcg gagccaatcc
      541 tccgatataa tgcccgggat ctgagccctg cagcacacat tctcgtgaac cgacaactct
      601 tgacagtgta cacccgtccg ttataacatg aacgcccgtt ccatgtccgg tgaatccaca
      661 tttacctgta tgtggacggt gatcgagaca aaaacggtca atacgcgagc attctagtac
      721 aagaaagcct gcattagtga gtgacaagtc gttgtaccgg ttcagtagta ccgatagtat
      781 cgccgacatg ggtcggcgag gcactcccgg ccatagctag tatttcatcc aatacctgac
      841 ttttaccagt acatgtataa ctacggccac acagtgtata aatgtcggtt gccgtctgct
      901 accaaataac gaaagtgacg cgcgaacatt actatcgatt gatctcgaac ccgatgaatt
      961 cagatgaatc gatattgatt gataccagcc atattaagcg ccgcggtcca gcgtgggcga
     1021 tcgctctaga gctagcgaat tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1861 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2161 agtatatatg agtaaaaata ttccggaatt gccagctggg gcgccctctg gtaaggttgg
     2221 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
     2281 atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     2341 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     2401 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     2461 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     2521 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     2581 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catcccacct
     2641 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     2701 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     2761 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     2821 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     2881 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     2941 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3001 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3061 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaaccgg
     3121 taatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3241 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3301 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3361 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3421 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3481 agagtgcacc aattggtcga cctcgagtta attaacgta
//
