LOCUS       sequence-173791-        3726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the three-armed star shown in figure 3D of associated 
            article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_wm_ruleB
FEATURES             Location/Qualifiers
     source          1..3726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1250..1272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1261..1277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1285..1291,1292..1309,1310..1315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1330..1351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1468..1485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1639..2227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1719..1738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2529..3323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2583..2602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3193..3212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3488..3506
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3544..3566)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3666..3685
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aagatcgcac aaattatcac gaatactgtg gaaagtagct agtatttcat ccaatagcag
       61 ttacacgtca gttacgtcga gcagaattcc tatactcatt catgttataa cggacgggta
      121 tagatagtga gggtctctaa tgtcccaagg aaaaagagca attcttccat cctggataga
      181 gtttgggcat gtaagatttt atcactgtaa actggttgca ccattataaa tgtcggttgc
      241 cgtcttcagt aaattcgaga tcaatcgata gtaattgtcc atgcctttat gcttgcgtta
      301 gaggacctat cacaagtaca agtcttactg tatatccgga ctaacaccgg ctctgaggtt
      361 gacgccacag agggtaccgc agccctcctt agcatagcta gccctaagtg agcttcaggc
      421 catgagacag ccaaggggtc agtcgcaata gtcggatgag cgtcaggggg tagtaggtat
      481 tagaggtgcg ctgtaggtat cgactgtatc atcagcggat catctcgatc ggagccaggt
      541 aaatgtggat tcaccggaga tctgagccct gtcaatacgc gagcattcta gttggctcat
      601 tgctcagtgt actgctaaac tcacccgata ggacttcagt agtaccgata gtatcgatag
      661 ttagcctgca ttagtgagtg acaacgagac aaaaacgttc gacgaaacgt tacgcctcca
      721 caagaattcg agatctatga attatgaggt cgttgtaccg ggaattcggc gcccacgcct
      781 aagccgacat gggtcggcga ggcactcccg gccagcaccg ccggagactt tacagcctga
      841 ctttttgaag tccatttagg accgcggcca cacagtgggg cgtacatgtt ttcaccaggc
      901 taccaaataa cgaaagtgac gcgcgaacaa tgtggggtac gatacaggct ggaccgcggc
      961 gcttaataat ggcgggatgt ttcaatatcg attcatctga attgaggcag gcatcgctaa
     1021 cacggaccgg agtgatatat aaaattacaa gtggctcatt ggcgaacgag gctgagaata
     1081 cccgtgggga caagggactg atcccaatcc aagagcgctg attacattac gtcctgtcaa
     1141 gagttgtcgg ttcacgagct aagagttcaa ttcagatgat cgctgtcgtt ctagcctaat
     1201 taccagtaca tgtataacta gtgggcgatc gctctagagc tagcgaattc ctgtgtgaaa
     1261 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1321 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1381 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1441 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2041 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaaaatatt ccggaattgc
     2401 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     2461 tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
     2521 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     2581 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2641 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     2701 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     2761 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     2821 cggggcagga tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg
     2881 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     2941 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3001 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3061 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3121 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3181 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3241 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3301 gccttcttga cgagttcttc tgaaccggta atattattga agcatttatc agggttattg
     3361 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3421 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     3481 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     3541 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     3601 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     3661 ctatgcggca tcagagcaga ttgtactgag agtgcaccaa ttggtcgacc tcgagttaat
     3721 taacgt
//
