LOCUS       sequence-173792-        3572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the Drigalski spatula shown in figure 3E of associated 
            article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_drigalski
FEATURES             Location/Qualifiers
     source          1..3572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     282..300
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(338..360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     460..479
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1616..1638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1627..1643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1651..1657,1658..1675,1676..1681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1696..1717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1834..1851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2005..2593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2085..2104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2895..3572,1..117)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2949..2968)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     join(3559..3572,1..6)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
       61 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaacc
      121 ggtaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
      241 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      301 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      421 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      481 tgagagtgca ccaattggtc gacctcgagt taattaacgt aacgtcagtt acgtcgagca
      541 gaatactgtg gaaatccaag agcgctgatt acattcgcag ttagtaagat tttatcactg
      601 taaagattcc tatactcaag aattttaacc tcgctggaca tagatagact ctatccagga
      661 tggaagaagg aaaaagagca tccggtccgt gttagcgatg cattgggcat cgccaatgag
      721 ccacttgtaa cctggttgca ccatcgtgca cagccggatc ctcagtcagt aaattggact
      781 ggactatccg agcggaacga cagcgatcat ctgaacgtta gaggacctcg tgataatttg
      841 tgcgatcttg tatatcatcg agatgatccg ctgatgagtt gacgccacag agggtaccgc
      901 agccctcctt agcatagcta gccctaagtg agcttcaggc caggtattag aggtgcgctg
      961 tatcgcaata gtcgttcgac gaaacgttac gcctccgtgt gtgttcgaga tctatgaatt
     1021 atgatgtaga ttcgtacgtg ggagattgtc ggagccaatc ctccgatata atgcccggga
     1081 tctcaatacg cgagcattct agtgggcccc agcctgcatt agtgagtgac aatgctaaac
     1141 tcaccttcag tagtaccgat agtatcgttg ggcttgatag ttaaccgtgt atgtggacgg
     1201 tgatcgagac aaaaacggtg tatacttgct agctagtatt tcatccaata ccacggtgtt
     1261 accagtacat gtataactag ggaattcggc gctataaatg tcggttgccg tcttcggcga
     1321 ggcactcccg gccagcaccg ccggagactt tacagcctga ctttttgaag tccattttac
     1381 tatcgattga tctcgaaggg cgtacattca gatgaatcga tattgaataa cgaaagtgat
     1441 attaagcgcc gcggtccagc gatacagaat cccgatgagg actgtcagat ggcgggatgt
     1501 tgggtaaatg tggattcacc ggagaggcag gtttcattca tgttataacg gacgggttat
     1561 aaaactcaag agttgtcggt tcacgagtgg gcgatcgctc tagagctagc gaattcctgt
     1621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa aatattccgg
     2761 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     2821 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     2881 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     2941 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3001 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3061 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3121 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     3181 aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa gtatccatca
     3241 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     3301 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     3361 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     3421 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     3481 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     3541 accgctatca ggacatagcg ttggctaccc gt
//
