LOCUS       sequence-173793-        4027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the cross shown in figure 3G in the associated article.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_cross
FEATURES             Location/Qualifiers
     source          1..4027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(183..205)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     305..324
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1916..1938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1927..1943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1951..1957,1958..1975,1976..1981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1996..2017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2134..2151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2305..2893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2385..2404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3195..3989
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3249..3268)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3859..3878
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
       61 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
      121 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
      181 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
      241 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
      301 tatgcggcat cagagcagat tgtactgaga gtgcaccaat tggtcgacct cgagttaatt
      361 aacgtaacgt cagttacgtc gagcagaata ctgtggaaag ttcatgttat aacggacggg
      421 tgcagttacg taagatttta tcactgtaaa attcctatac tcaagaattt taacctcgct
      481 ggacatagat agtgagggtc tctaatgtcc caaactctat ccaggatgga agaatacaat
      541 cccacatccg gtccgtgtta gcgatgctcc acggatcgcc aatgagccac ttgtaaagcc
      601 ggatcctcag tcagtaaatt ggactggact atccgagcgt tgtccatgcc tttatgcttg
      661 cgttagagga cctatcacaa gtacaagtct tactgtatat ccggactaac accggctctg
      721 aggttgacgc cacagagggt accgcagccc tccttagcat agctagccct aagtgagctt
      781 caggccatga gacagcgata ctatcggtac tactgaacgg atgagcgtca ttgtcactca
      841 ctaatgcagg ctaccaacga ctagaatgct cgcgtattga acgtgggaga ttgtcggagc
      901 caatcctccg atataatgcc cgggatctga gccctgcagc acagaacgac agcgatcatc
      961 tgaattgctc agtgtatcgt gataatttgt gcgatctacg cccgtatcga gatgatccgc
     1021 tgatgataac cgtgtatgtg gacggtgatc gagacaaaaa cggtgtatac ttgcatagaa
     1081 ctccacaaga aattgccacg gtggcggcaa tcggtcgttg taccgggaat tcggcgccca
     1141 cgcctaagcc gacatgggtc ggcgaggcac tcccggccag caccgccgga gactttacag
     1201 cctgactttt tgaagtccat ttaggaagac ggcaaccgac atttatatac atgttttcac
     1261 tagttataca tgtactggta atgacgcgct attggatgaa atactagcta agaatcccga
     1321 tgaggactgt cagatggcgg gatgttgata ccagccatgc tgcgaaatga ggcaggtatt
     1381 agaggtgcgc tgtatggtga tatatattcg acgaaacgtt acgcctctgt acgagttcga
     1441 gatctatgaa ttatgaaagg gactgatccc aacgcgaccg caaaccctgt gaaacgtcct
     1501 gccctattcc acgacaacta aagctaagag ttcaagtgca tctggtctgc cgcaattagc
     1561 ctaatttaat tctgaaactt agccagggta aatgccatat cccccgtaca gattgcttca
     1621 tttggaggta catttctgtg atcagatttg tccggtgctg gaccgcggcg cttaatatgg
     1681 caacattccc tcaatatcga ttcatctgaa tccttcgtat tcgagatcaa tcgatagtaa
     1741 cagtgccagg taccaagacg cctttgttca atatgagagg gcaataccgg cgtaagccag
     1801 acaggtaaat gtggattcac cggaattact ggtcggtcca agagcgctga ttacattatg
     1861 tgatgtcaag agttgtcggt tcacgagtgg gcgatcgctc tagagctagc gaattcctgt
     1921 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1981 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2101 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa aatattccgg
     3061 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     3121 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     3181 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3241 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3301 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3361 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3421 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     3481 aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa gtatccatca
     3541 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     3601 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     3661 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     3721 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     3781 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     3841 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     3901 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     3961 tctatcgcct tcttgacgag ttcttctgaa ccggtaatat tattgaagca tttatcaggg
     4021 ttattgt
//
