LOCUS       sequence-173794-        4031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the straight four-helix-bundle shown in figure 4A of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_4hb_straight
FEATURES             Location/Qualifiers
     source          1..4031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1554..1576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1565..1581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1589..1595,1596..1613,1614..1619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1634..1655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1772..1789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1943..2531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2023..2042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2833..3627
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2887..2906)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3497..3516
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3792..3810
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3848..3870)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3970..3989
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tattggatga aatactagct aatactgtgg aaaggctaga ggtctgctcg acgtaactga
       61 cgtgagaact aacgatagca tcggttacca gtacatgtat aactaaacct cgctggacat
      121 agatagtcca agagcgctga ttacattgga aaaagagcaa ctcgtatgtt cgagatctat
      181 gaattatgag acgaaaacgc ctccacggac tataaatgtc ggttgccgtc tgccggatcc
      241 tcagtcagta aattggactg gactatccga gcgttgtcca tgcctttatg cttgcgttag
      301 aggacctatc acaagtacaa gtcttactgt atatccggac taacaccggc tctgaggttg
      361 acgccacaga gggtaccgca gccctcctta gcatagctag ccctaagtga gcttcaggcc
      421 atgagacagc ctcatcagcg gatcatctcg atggatgagc gtcagggggt agtaggtatt
      481 agaggtgcgc tgtaggtatc gactgtagat tcgtattcat gttataacgg acgggtatcc
      541 tccgatataa tgcccggact ctatccagga tggaagaaat tcgggcccca gtggctcatt
      601 cgtgaaccga caactcttga cacccgatag gacgcccgtt cggtaaatgt ggattcaccg
      661 gaaccgtgta tgtggacggt gatcgagaca aaaacggtgt atacttgcat agaactccac
      721 aagaaattgc cacggtggcg gcaatcggtc gttgtaccgg gaattcggcg cccacgccta
      781 agccgacatg ggtcggcgag gcactcccgg ccagcaccgc cggagacttt acagcctgac
      841 tttttgaagt ccatttagga ccgtaagatt ttatcactgt aaacatgttt tcaccaggct
      901 accatccggt ccgtgttagc gatgcacaat gtggggtacg atacagacta gaatgctcgc
      961 gtattgaatg gcgggatgtt gataccagtt cagtagtacc gatagtatcg tttcaaacat
     1021 atactgcatg ttcgagatca atcgatagta acctttgggc gtgtacgagg ctttcgacga
     1081 aacgttacgc ctcggactga tcccaacgcg accgcaaacc ctgtgaaacg tcctgcccta
     1141 ttccacgaca actaaagcta agagttcaag tgcatctggt ctgccgcaat tagcctaatt
     1201 taattctgaa acttagccag ggtaaatgcc atatcccccg tacagattgc ttcatttgga
     1261 ggtacatttc tgtgatcaga tttgtccggt aggtcaatat cgattcatct gaatcaacat
     1321 tcccattacc gtcgggctgg accgcggcgc ttaatacgct gaccaacagc cccgcagtta
     1381 caagtggctc attggcgatg ttcaatatga gagggcaata gatcgcacaa attatcacga
     1441 agaacattgg tggcatacat tttgtcactc actaatgcag gcaagctgca tatgtgatga
     1501 cttgaacgac agcgatcatc tgaagtgggc gatcgctcta gagctagcga attcctgtgt
     1561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1621 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1681 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaaaa tattccggaa
     2701 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     2761 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga
     2821 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     2881 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     2941 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3001 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     3061 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3121 gtgccggggc aggatctcct gtcatcccac cttgctcctg ccgagaaagt atccatcatg
     3181 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     3241 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3301 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     3361 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     3421 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     3481 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     3541 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     3601 tatcgccttc ttgacgagtt cttctgaacc ggtaatatta ttgaagcatt tatcagggtt
     3661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3721 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     3781 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     3841 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     3901 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     3961 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccaattggtc gacctcgagt
     4021 taattaacgt a
//
