LOCUS       sequence-173795-        4137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the bent four-helix-bundle shown in figure 4E of 
            associated article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_4hb_bent
FEATURES             Location/Qualifiers
     source          1..4137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    41..57
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(65..71,72..89,90..95))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(248..265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(419..1007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(499..518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1309..2103
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1363..1382)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1973..1992
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2268..2286
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2324..2346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2446..2465
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgggcgatc gctctagagc tagcgaattc ctgtgtgaaa ttgttatccg ctcacaattc
       61 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      841 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1141 caatctaaag tatatatgag taaaaatatt ccggaattgc cagctggggc gccctctggt
     1201 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     1261 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     1321 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     1381 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     1441 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     1501 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     1561 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     1621 tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     1681 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     1741 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     1801 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     1861 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1921 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1981 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     2041 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     2101 tgaaccggta atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     2161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     2221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2281 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2341 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2401 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2461 ttgtactgag agtgcaccaa ttggtcgacc tcgagttaat taacgtatat tggatgaaat
     2521 actagctaat actgtggaaa ggctagaggt ctgctcgacg taactgacgt gagaactaac
     2581 gatagcatcg gttaccagta catgtataac taaacctcgc tggacataga tagtccaaga
     2641 gcgctgatta cattggaaaa agagcaactc gtatgttcga gatctatgaa ttatgagacg
     2701 aaaacgcctc cacggactat aaatgtcggt tgccgtctgc cggatcctca gtcagtaaat
     2761 tggactggac tatccgagcg ttgtccatgc ctttatgctt gcgttagagg acctatcaca
     2821 agtacaagtc ttactgtata tccggactaa caccggctct gaggttgacg ccacagaggg
     2881 taccgcagcc ctccttagca tagctagccc taagtgagct tcaggccatg agacagcctc
     2941 atcagcggat catctcgatg gatgagcgtc agggggtagt gcgtgtgtgt taggtattag
     3001 aggtgcgctg tatagattcg tacgtgggag attgtcggag ccattcatgt tataacggac
     3061 gggtgatctg agccctgcag cacacattcg ggccccactc tatccaggat ggaagaactg
     3121 ctaaactcac ccgataggac gcccgttctc gtgaaccgac aactcttgaa ccgtgtatgt
     3181 ggacggtgat cgagacaaaa aggtaaatgt ggattcaccg gatccacaag aaattgccac
     3241 ggtggcggca atcggtcgtt gtaccgggaa ttcggcgccc acgcctaagc cgacatgggt
     3301 cggcgaggca ctcccggcca gcaccgccgg agactttaca gcctgacttt ttgaagtcca
     3361 tttaggaccg cggccacaca gtggggcgta catgttttca ccaggctacc aaataacgaa
     3421 aggtaagatt ttatcactgt aaatacgata cagaatcccg atgaggactg tcagatccgg
     3481 tccgtgttag cgatgccatg ctgcgaaatg aggcaggttt caaacatact agaatgctcg
     3541 cgtattgaaa cgtaagtacc tttgggcgtg tacgaggctg ttcagtagta ccgatagtat
     3601 cgactgatcc caacgcgacc gcaaaccctg tgttcgagat caatcgatag taagacaact
     3661 aaagctaaga gttcaagtgc atctgttcga cgaaacgtta cgcctcttaa ttctgaaact
     3721 tagccagggt aaatgccata tcccccgtac agattgcttc atttggaggt acatttctgt
     3781 gatcagattt gtccggtagg ccgtggttta gatggaaatg gcaacattcc cattaccgtc
     3841 ggtgaagcct caccttcgta taccgctgac caacagcccc gcagtgccag gtaccaagac
     3901 gccttttcaa tatcgattca tctgaatccg gcgtaagcca gacatgaagc tggaccgcgg
     3961 cgcttaatac tggtcggtag ttactatcga ttacaagtgg ctcattggcg acgaagactg
     4021 ttttagtcaa atagatcgca caaattatca cgatcccgag cccagacggg cttattgtca
     4081 ctcactaatg caggcgaatt gtttttagct acgctcgaac gacagcgatc atctgaa
//
