LOCUS       sequence-173802-        7783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral stable expression of Myc-TZAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pLPC-Myc-TZAP
FEATURES             Location/Qualifiers
     source          1..7783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             12..481
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(611..628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(862..881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1530..2321,2322..2390))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2153..2172
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2391..2495)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2526..2995
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3060..3259
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3460..3876
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3806..3828
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3844..3860
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3894..4493
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3894..3913)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4390..4410
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4582..4601)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4773..5152
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5153..5356
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5307..5327
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5401..5420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5401..5419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5451..5460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5460..5489
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
       61 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
      121 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca
      181 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
      241 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
      301 ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg
      361 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
      421 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
      481 acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc
      541 catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1441 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
     1501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2521 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     2581 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     2641 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     2701 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2761 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2821 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     2881 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     2941 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
     3001 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
     3061 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
     3121 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     3181 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     3241 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     3301 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     3361 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     3421 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     3481 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     3541 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3601 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3661 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3721 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3781 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3841 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcagctt accatgaccg
     3901 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     3961 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4021 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     4081 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     4141 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     4201 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     4261 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     4321 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     4381 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     4441 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     4501 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gatctcccga
     4561 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     4621 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     4681 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     4741 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     4801 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4861 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4921 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4981 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     5041 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     5101 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     5161 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     5221 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5281 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     5341 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     5401 taatacgact cactataggg agacccaagc ttggtaccga gctcggatca gccaccatgg
     5461 aacaaaagtt gatttctgaa gaagatttgt taagaaagag aggatccatg gacggctcct
     5521 tcgtccagca cagtgtgagg gttctgcagg agctcaacaa gcagcgggag aagggccagt
     5581 actgcgacgc cactctggac gtggggggcc tggtgtttaa ggcacactgg agtgtccttg
     5641 cctgctgcag tcactttttc cagagcctct acggggatgg ctcagggggc agtgtcgtcc
     5701 tccctgctgg cttcgctgag atctttggcc tcttgttgga ctttttctac actggtcacc
     5761 tcgctctcac ctcagggaac cgggatcagg tgctcctggc agccagggag ttgcgagtgc
     5821 cagaggccgt agagctgtgc cagagcttca agcccaaaac ttcagtggga caggcagcag
     5881 gtggccagag tgggctgggg ccccctgcct cccagaatgt gaacagccac gtcaaggagc
     5941 cggcaggctt ggaagaagag gaagtttcga ggactctggg tctagtcccc agggatcagg
     6001 agcccagagg cagtcatagt cctcagaggc cccagctcca ttccccagct cagagtgagg
     6061 gcccctcctc cctctgtggg aaactgaagc aggccttgaa gccttgtccc cttgaggaca
     6121 agaaacccga ggactgcaaa gtgcccccaa ggcccttaga ggctgaaggt gcccagctgc
     6181 agggcggcag taatgagtgg gaagtggtgg ttcaagtgga ggatgatggg gatggcgatt
     6241 acatgtctga gcctgaggct gtgctgacca ggaggaagtc aaatgtaatc cgaaagccct
     6301 gtgcagctga gccagccctg agcgcgggct ccctagcagc tgagcctgct gagaacagaa
     6361 aaggtacagc ggtgccggtc gaatgcccca catgtcataa aaagttcctc agcaaatatt
     6421 atctaaaagt ccacaacagg aaacatactg gggagaaacc ctttgagtgt cccaaatgtg
     6481 ggaagtgtta ctttcggaag gagaacctcc tggagcatga agcccggaat tgcatgaacc
     6541 gctcggaaca ggtcttcacg tgctctgtgt gccaggagac attccgccga aggatggagc
     6601 tgcgggtgca catggtgtct cacacagggg agatgcccta caagtgttcc tcctgctccc
     6661 agcagttcat gcagaagaag gacttgcaga gccacatgat caaacttcat ggagccccca
     6721 agccccatgc atgccccacc tgtgccaagt gcttcctgtc tcggacagag ctgcagctgc
     6781 atgaagcttt caagcaccgt ggtgagaagc tgtttgtgtg tgaggagtgt gggcaccggg
     6841 cctcgagccg gaatggcctg cagatgcaca tcaaggccaa gcacaggaat gagaggccac
     6901 acgtatgtga gttctgcagc cacgccttca cccaaaaggc caatctcaac atgcacctgc
     6961 gcacacacac gggtgagaag cccttccagt gccacctctg tggcaagacc ttccgaaccc
     7021 aagccagcct ggacaagcac aaccgcaccc acaccgggga aaggcccttc agttgcgagt
     7081 tctgtgaaca gcgcttcact gagaaggggc ccctcctgag gcacgtggcc agccgccatc
     7141 aggagggccg gccccacttc tgccagatat gcggcaagac cttcaaagcc gtggagcaac
     7201 tgcgtgtgca cgtcagacgg cacaaggggg tgaggaagtt tgagtgcacc gagtgtggct
     7261 acaagtttac ccgacaggcc cacctgcgga ggcacatgga gatccacgac cgggtagaga
     7321 actacaaccc gcggcagcgc aagctccgca acctgatcat cgaggacgag aagatggtgg
     7381 tggtggcgct gcagccgcct gcagagctgg aggtgggctc ggcggaggtc attgtggagt
     7441 ccctggccca gggcggcctg gcctcccagc tccccggcca gagactgtgt gcagaggaga
     7501 gcttcaccgg cccaggtgtc ctggagccct ccctcatcat cacagctgct gtccccgagg
     7561 actgtgacac ataggaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
     7621 ctagagggcc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     7681 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     7741 atacataact gagaatagag aagttcagat caaggtcagg aac
//
