LOCUS       sequence-173804-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic 
            stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pPHAGE2-Myc-mTZAP
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        184..1362
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          415..1353
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1310..1330
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1375..1404
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3478..3976
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3513..3536)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3909..3928
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4016..4615
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4016..4035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4512..4532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4629..5217
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4682..4702)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5100..5111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5365..5545
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5592..5610)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5678..5782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5783..5851,5852..6643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6001..6020)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6814..7402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7556..7573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7813..7834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7849..7854,7855..7872,7873..7879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7887..7903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7892..7914
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7911..7927
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7911..7927
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7977..7996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8188..8821
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8868..8993
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9490..9723
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9908..9952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      181 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      241 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      301 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      361 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      421 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      481 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      541 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      601 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      661 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      721 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      781 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      841 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      901 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      961 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1021 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1081 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1141 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1201 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1261 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1321 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccg catggaacaa
     1381 aagttgattt ctgaagaaga tttgttaaga aagagaggat ccgacggttc cttcgtgcag
     1441 cacagcgtga gggttctgca ggaactcaac aagcagcgcg agaagggcca gtattgcgac
     1501 gccacactgg acgtgggggg cctggtgttc aaggcgcact ggagtgtcct ggcgtgttgc
     1561 agccatttct tccagagaat ctacggggat ggcacagggg gcagcgttgt cctcccagcg
     1621 ggctttgctg agatttttgg cctcctgctg gactttttct acactggcca cctcgccctc
     1681 acctcaggga acagggatca ggtactcttg gcagccaaag agttgagggt gcctgaggct
     1741 gtggagctat gtcagagctt ccagccccaa acctcagtgg gacaggcaca gagtggactg
     1801 ggtcaacctg cctcccagga cgtgaaaagc cacctgaagg agccgacaga cttggatgag
     1861 gaggaagttt tcaggactct gagcctggcc tccgtggatc aggagcccag agacactgag
     1921 cagccccagc tgggtacacc tgctcagagc accacggcct tcctctgtgg gaaactcacg
     1981 caggctttga agccttctcc ctcagaggac aaagagtctg aggactgcaa agagccccca
     2041 aggcccttcg aggctggagg tgccccgctg cagggcgaga gcaatgagtg ggaagtggtg
     2101 gtccaagtgg aggacgacag ggacggtgat tacgtatctg agcctgagac tgtgctaacc
     2161 agaaggaagt caaaagtgat tagaaaaccc tgtgcagccg agccagccct gggtgcgggt
     2221 tctctcacag ccgagcccac tgacagcaga aaaggtgcag ccgttccggt cgaatgtccc
     2281 acatgtcaca aaaagttcct cagcaaatat tacctgaaag tccacaacag gaaacacact
     2341 ggggagaaac ccttcgagtg tcccaaatgt gggaagtgtt acttccgcaa ggagaatctc
     2401 ttggaacatg aagccaggaa ttgcatgaac cgctcagaac aggtcttcac atgctccgta
     2461 tgccaggaga ccttccgacg gaggatggag ctgcgcttgc acatggtgtc tcacacaggg
     2521 gagatgccct acaagtgttc ctcctgctcc cagcagttca tgcagaagaa agacttgcag
     2581 agccacatga tcaagctgca cggagcccct aaaccccacg catgccccac ttgtgccaag
     2641 tgcttcctgt ctaggacgga gctacagctg cacgaggctt ttaagcatcg tggagaaaag
     2701 ctctttgtgt gtgaggaatg cgggcaccgg gcctcgagcc gcaacggact gcagatgcac
     2761 atcaaggcca agcacaggaa tgaaaggcct tatgtctgtg agttctgcag ccatgccttc
     2821 acccagaagg ccaacctcaa catgcacctg cgcacacaca ccggcgagaa gcctttccag
     2881 tgccacctct gtgggaagac cttccgcacc caagccagtt tggacaagca caaccgcacc
     2941 cacacgggcg agaggccttt cagctgtgaa ttctgtgagc agcgcttcac tgagaagggc
     3001 ccccttctga ggcatgtggc cagccgccat caagagggca ggccccactt ctgccagatc
     3061 tgtggcaaga ccttcaaagc tgtggaacag ttacgagtgc acgtcaggcg acacaaaggt
     3121 gtgagaaagt tcgagtgcac cgagtgtggc tacaagttca cgcggcaggc tcacctgcgg
     3181 aggcacatgg agatccacga cagagtggag aactacaatc cccggcagcg caagctccgg
     3241 aacctcatca ttgaggatga gaagatggtg gtcgtggctc tccagccccc tgcagacctg
     3301 gaggtgggct ctgctgaggt cattgtggag tccctgaccc agggtggcct tgcctcccaa
     3361 ctccctagcc agagactgtg ttcagaggag agctttgcta gccctggcgt cctggagccc
     3421 tctctcatca tcaccgctgc tgttcccgag gactgtgaca cgtagccatg gggatccggg
     3481 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     3541 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     3601 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     3661 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     3721 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     3781 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagcagctg ggaagggtgg
     3841 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     3901 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     3961 atctccgggc ctttcgacct gcatccatct agatctcgag cagctgaagc ttaccatgac
     4021 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4081 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4141 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4201 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4261 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4321 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4381 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4441 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4501 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4561 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaatcga
     4621 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4681 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4741 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4801 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4861 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4921 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4981 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5041 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5101 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5161 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5221 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5281 aaggggggac tggaagggct aattcactcc caacgaagac aagatcacct gcaggacagg
     5341 cgcgccctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5401 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5461 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5521 tagtcagtgt ggaaaatctc tagcacccgg gcgattaagg aaagggctag atcattcttg
     5581 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5641 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5701 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5761 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5821 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5881 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5941 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6001 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     6061 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6121 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6181 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6241 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6301 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6361 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6421 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6481 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6541 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6601 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6661 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6721 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6781 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6841 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6901 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6961 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7021 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7141 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7201 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7261 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7321 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7381 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7441 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7501 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7561 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7621 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
     7681 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7741 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
     7801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7921 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7981 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     8041 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     8101 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     8161 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     8221 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     8281 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     8341 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     8401 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     8461 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     8521 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     8581 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     8641 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8701 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8761 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8821 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     8881 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     8941 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9001 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9061 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     9121 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     9181 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     9241 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9301 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     9361 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9421 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9481 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9541 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9601 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9661 catcaagcag cttcaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9721 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9781 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9841 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9901 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9961 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10021 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10081 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
    10141 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    10201 tcgacggtat cgccgaattc acaaat
//
