LOCUS       sequence-173819-        8792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab44 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..27)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     218..237
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     450..472
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     464..481
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     465..481
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     488..507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        488..506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6421..6439)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6422..6439)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6457..6473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6457..6473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6470..6492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6481..6497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6505..6511,6512..6529,6530..6535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6550..6571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6688..6705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6859..7447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6939..6958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7618..8409,8410..8478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8241..8260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8479..8583)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(8661..8792,1..324))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
       61 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      121 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      181 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      301 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
      361 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      421 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      481 tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg ctcccggccg
      541 ccatggcggc cgcgggaatt cgattggcat cagtgtcatt ccaagcccag ttgttcagag
      601 aagaaaaata ggctcaactc gaagacctct aaaagggaat aatgtgactg cagagtcaga
      661 tgtgcttcaa aaagccactt tagctgatgc aaaattagaa aaagacaatg tatctgaaaa
      721 tactgagagc ctttcagctg tcaccgattc taatctttta cttcaacatc atggcactga
      781 aggagcagga gaaacagact caattcctaa acaaagtggc atcagcatca tccctggaga
      841 tcttcaaaga aaggagacaa acttagagtc aagtccagtt attcagagaa gaaaaatggg
      901 ctcaactcga agacctctca aaggaaataa aggacaaaga aaaggacgag agcttcatga
      961 tgaaaatgaa acatttaaca gtgaattttt gcttgatgta cagaaagaaa cattcggttt
     1021 ggaggatgaa ccctgtccag atgaatctac attacaaaac ataaaagatg gagctgaaga
     1081 acaggagaag gaactgtgtg tgaagaatga tgctagtctt gttggagagt ctgaatctgc
     1141 tctcattcag tcatcatacg gttcagattt agcgtctgga gaaaccactg cattgagtgt
     1201 ctgtaacatc acagaaccac aaaccctcac aaaaactcac gaagaggcct gggaaatagt
     1261 tgggggtgtt gaaagagcaa cagtagaaga agacacagac ttgcaatcaa aaaccgagtc
     1321 acaaaaccaa acatgtgcct caattagtga agcagaagaa aacatccatg atgtaaatgt
     1381 tttagtcaga gaagctgaag ttactggaga atcagttata ttggatagta atgtgactgc
     1441 agagtcagat gtgattcaaa aagacacttt agctgatgca gaattagaaa cattgcaagt
     1501 ttctgaaaat acagaggatg tttctgctgt gactgatact gattttctag tgcaacatca
     1561 tggcactgaa cgagcagaag ggacagacac aatttctgta caaagtggca tcaacatcat
     1621 tcctagagag tctcacggag aagaggcgaa ctcaaagtcg agtccagttg ttcagaggag
     1681 aaaaatgggc tcaactcgaa gacctctcaa aggaaataaa ggacaaagaa aaggacaaga
     1741 gcttgatgat gaaaaggaaa catttcactg tgaatttttg cttgatgtag aaaaagaaac
     1801 atttagtttg gagaatgaac cctgtccaga tgaatctaca ttacaaaaca tgaaagatgg
     1861 agctgaagaa caggagaagg aaatatgtct ggagaatgat gttggtcttg ttgaagagtc
     1921 tgaatctgct ctcattcagt catatagttc agatttagtg tctgaagaat ccactgcatt
     1981 gagtgtctgt aacatcacag aatcacaaac cctcacaaaa attcaagaag agtcctggga
     2041 aatagttgga gatgttgaaa gagaatcagt agatggagat acagacttac aattagaaac
     2101 agagtcacaa aaccaaacat gtgactcaac cagtaaagca gaacaaaaca tccatgatgt
     2161 aaatgtatta gtcagagaag ctgaaggtat tggggaatca gttaaagtgg acagcaatgt
     2221 gattgcagag tcagatgtgc ttcaaaaagc cactttagct gatccaaaat tggaaacatt
     2281 gcacgtatct gaaaatacta aggatgtttc tgctgtcacg gattctgatc ttctagtgca
     2341 acatgaacca actgtagaag caggagaaac agacacggtt cctgaaaaaa gtggaatcag
     2401 catcattcct ggagattctc acaacgaaga ggcaaactca aagtcaagtc cagttgttca
     2461 gaggagaaaa atgggctcaa ctcgaagacc tctcaaagga aataaaggac aaagaaaagg
     2521 acgagagctt caagatgaaa atgagacact taataatgaa attaggtttg atgtagagaa
     2581 agaaacattt ggtttggaga atgaaccctg tccagatgaa tctgcattac aaaacataaa
     2641 agttggggct gaagaacagg agaaggaaat atgtgtgaag aatgatgctg gtgttgttga
     2701 agagtatgaa tctgatctca ttcagtcatt ttatggttcg gatttagtgt ctgaagaatc
     2761 cactgcatta agtgtctgcc acatcgaaga agcacaaact ctcacaatta gtcatgaaca
     2821 atttcagaat aatcctggag atgcagaaag agcagcagta gttgaagatg ctgccgatac
     2881 tgaagatact tcagaaactg aagctacagt tacaatgaag agcactacaa atactgcatc
     2941 acatgttttt caaacgtctc ctagttcaag cacagatgca tttaatatat ctacaaatgt
     3001 cgaggaagat acttcaggag acacagacac aattttcaag cagagtaaca tcaacgttgt
     3061 tcatgaagat atttatagag gggaagctca cccaaagtta agtccagttg ttcagaagag
     3121 aaaaatgggc tcaactcgaa gacctctcag ggggaacaaa ggacaaaaaa aagataatga
     3181 tgaaaaggaa atctcatttc tggaaaatga acccaatcca gatgagctta tatacactgc
     3241 aatgggtatc agtgatattt cagaagctca aacccacgta aaagttcacg aagagtccca
     3301 ggacaaccat gaagttgttg aaaaagcaac agtaggggaa agcacacaat cacaaacaga
     3361 aaacaaagtg agtgtgttag tcagagaagc tgaagttcct gaaaatacag ttgtgattaa
     3421 tacaactgag gagtcgtatt tgtttcaata cgtctctgat tcaaaactga ccatatctga
     3481 ctatgaacat gaggctccag taattccaaa cataatttcc aaacaaagtg acttcagcat
     3541 tcctggagat tctcacaaag aagaggaaaa ctcacaatta agtccagttg ttcaaaagag
     3601 aaaaatgggc tcaactcgaa gacctctcaa agggaataag gggcaaggaa agcagagaga
     3661 agaatccaag gtaaagaata gagataatga ggcagggtca gaagaggaaa acacagaaac
     3721 acattcaaaa gaccaggaac aaaatgtggc aaaagacaat atgaatataa tagataatgt
     3781 caatgaagaa aaaaaggcaa caaaggcatt ggatacatcc atgcaggaac tatgtgaaca
     3841 agagcaaaga tcagatgtat cacaaacaca tgctgataca tcacaggatg aaaccagctc
     3901 acatttattg gacatcccag aaagaactgt agattttgtt agggagtcta cgagtcagcc
     3961 tgatcttgtt ctgtctgatg tgttgcttat gacagaagct caaacgtatt tgtcatcaga
     4021 agataatgtc atgggtaaca gttcaatatc tataacagag gtacatgaaa tgcatgcaga
     4081 ctccaataca tccaagagaa aaatggggtc aactagaaag agaggaaaga ggatggtgaa
     4141 tgaagaggaa gaggaagctg aaaatacaat tggttctgga gccacaagtt ccaaggagac
     4201 attaactcct aaaaaattaa ttgcagaatc ttctgtacat aaaccactgt ttagttcata
     4261 tgttattaac gaagaccatg acagtaaatc agatgtgcag actgagctct cagatcaaga
     4321 ttatctaaat tctaccataa acaccgaacc aatgaaagaa agagaaagtt ctccagatgt
     4381 tgttgctgaa atggtttctg actttgcttt gggacatgtt gacactgaag tagttcctga
     4441 gcaaagggtc attcctgaag agccacataa agatcaagca aacctgaatc cagttgttca
     4501 aaaacgaaaa atggggtcaa cacgtaaaac tcttagaggg aacaaagggc agagaaaaga
     4561 ggaagaatcg aaagaaatta atgaaggaat gacacaggat cggagtagag aactacattt
     4621 ggcaagtgaa agtaaacttg gtgaagaggt tcaacaaaac caatctctta ttaatatgac
     4681 aaacaataaa gaagctaaga aaccggagga tgaattggat gaaatgcctt cagcatcaag
     4741 catctctcaa ttcacagaag catcctcaga aactctcagt tcctcatatc ttaaatcaga
     4801 aaagttagat tcaaaagatg agaatgatta tcatgatact tgtgaacatg aaaatgccca
     4861 tcagatttgc gctgatgcta ccaaaagtct acagtcggaa tctgttccca ttgagaggag
     4921 aaggaagatg ggctcgaccc ggaaaaatat tggaagagaa ggaagaatgc aaagaaagcg
     4981 agatggatat gaggacacag aaatcataga cacaaatctg cagatgtctc acgaccctga
     5041 aatgagctca tcaggtataa ctgcagaaga acaaacaact gaagattact ccaatcagtc
     5101 agaggattca cttcttggtg aaatacagcg aatgctaaaa acaacttctc aaagtgcatc
     5161 caattttgaa ttgattattg atgagccgaa atcaaattca ccagaaggct cagaaatcag
     5221 atctctttca ccatcacaga gcttgacaac tctaaatgat ccaaactctc cagaaagacg
     5281 acggcgaaaa atgggttcaa ctcgcaagaa ccctaaacgg cagttcaaag cagaaagaga
     5341 agatgaagac aaagtagatc agaagacaga gtcaaacaaa gaagaaataa cggaggggtc
     5401 gctagtgcat aatatcacag aagaccctcc aaataaagag tatcttgatg ttcccagtgc
     5461 acacacaagc agccagcaag aagagagcga tagccctgtt gttcaagaaa gtgcgtcacc
     5521 atcaactaaa aggaagtttg ggtccagacg agctaataag ggcaaacttg gtaggcttga
     5581 ggcatctgat ggtatgactg aatcagaaga tggagatcat aaaccagatg gctctgctgt
     5641 tgaggacaga cagcacaggg atggttcaat gggttgtggt acgagcgtca gcttacaacc
     5701 agaaaatctg ccattttcca gaaattatga agttgctcca aaaggcactg gtgaagagat
     5761 tcaagtcaga aaacaggaca gaatgacggt caatgtaaag aacagggatt ctaaagtggt
     5821 tcagtttaat gtggtcatgg tgggaaacag ctgtgttgga aagacgtcct tcataagacg
     5881 tttccatgaa ggccagttca ccgaggatta ccgctccact attggtgttg acactttcgt
     5941 acagactgtt gagctgcccg acagaactgt taaactgcag atatgggaca cagcaggtca
     6001 agagaggttt cacagcatca ccacgcaagt tttccacaag gctgaggggc ttctgctcat
     6061 gtatgagatc acatgttcaa agagtttcat ttctatacga gattggatct ctcaagcccg
     6121 ggaaagggct caagatgatg tcgtcatgat gttgctcgga aacaagaatg attcagttaa
     6181 tcgtgaagtc cagattcagg aaggagcaga tctggccaga gagtataaca tacatttcat
     6241 ggagtgcagt gctgcaaacg gggctaatgt gtcagaatct atgaggactc ttgctgagtt
     6301 gttagtgcag cgcaagagaa aaagggagga acacacaacg aatcactagt gaattcgcgg
     6361 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat
     6421 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7021 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7261 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7501 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7561 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7621 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7681 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7741 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7801 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7861 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7921 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7981 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8041 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8101 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8161 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8221 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8281 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8341 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8401 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8461 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8521 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8581 gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa
     8641 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8761 atcaaaagaa tagaccgaga tagggttgag tg
//
