LOCUS       sequence-173824-        3716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab26 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..27)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     218..237
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     450..472
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     464..481
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     465..481
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     488..507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        488..506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1345..1363)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1346..1363)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1381..1397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1381..1397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1394..1416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1405..1421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1429..1435,1436..1453,1454..1459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1474..1495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1612..1629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1783..2371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1863..1882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2542..3333,3334..3402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3165..3184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3403..3507)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(3585..3716,1..324))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
       61 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      121 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      181 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      241 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      301 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
      361 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      421 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      481 tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg ctcccggccg
      541 ccatggcggc cgcgggaatt cgattatgga gaacgagccg gagcggagca gcggagctct
      601 gcgggatctg cggcagcggc ccgggaatca gagctgcgcg gactgcggtg ctgcagggct
      661 gattgcgtgt gcatggtctg aaagcggatc tgagggatgt gtgctgttgt tctggtgtct
      721 ggtgtctgac ggacagcagc acttgctgat ggttcagctg tcgggacggc tgacgctgca
      781 cagcacaaca caagagcaga ataaagtgct gagcatcgac agcatgaggg ttaaactaca
      841 gatctgggat acagcgggac aggagcgttt caggagtgtg acgcacgcat actatcgaga
      901 cgctcacgct cttttgctgc tttatgatgt gacaaataaa tcctcctttg acaacataca
      961 ggcctggctg acggagattc acgagttcgc tcagcagaac gtggtcatta tgctgttggg
     1021 taataaggct gatgctacac atgaacgcat tgtgaagagg gaggaggggg agaaactagc
     1081 taaggagttt ggcgttccct tcatggagac cagtgctaaa acaggcctga atgtggagtt
     1141 ggccttcact gcggttgcca aggaactgaa gcacagggag ttaaaggaac caaacgagcc
     1201 caagtttcag ctgcaggagt acgtgaacaa ggagatgaag ggagtcgggt gctgtcgctc
     1261 ataaaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac
     1321 gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
     1381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgatgcggt
     3541 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     3601 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3661 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtg
//
