LOCUS       sequence-173832-        9848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-4
FEATURES             Location/Qualifiers
     source          1..9848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..161)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(301..960)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     875..894
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(961..1063)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1987..2206
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2297..3052
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3055..3305
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3631..4806
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4806..5777
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5850..6323
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6583..6982
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     primer_bind     complement(7124..7143)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7243..7265
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7303..7321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7389..7493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7494..7562,7563..8354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7712..7731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8525..9113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9014..9033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9267..9284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9384..9782
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9800..9833)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taatacgact cactataggg agaagcttgc tgaggacact ctttccctac acgacgctct
       61 tccgatcttc atccacgtgc atgctggatc cgatacagat cggaagagca cacgtctgaa
      121 ctccagtcac atcctcagcg atcctatagt gtcacctaaa tcgtatgcgg ccgcccgggc
      181 cgtcgaccaa ttctcatgtt tgacagctta tcatcgaatt tctgccattc atccgcttat
      241 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
      301 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      361 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      421 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      481 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
      541 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      601 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      661 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      721 accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg
      781 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      841 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      901 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      961 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1021 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     1081 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
     1141 gtgatcttcc gtcacaggta tttattcgcg ataagctcat ggagcggcgt aaccgtcgca
     1201 caggaaggac agagaaagcg cggatctggg aagtgacgga cagaacggtc aggacctgga
     1261 ttggggaggc ggttgccgcc gctgctgctg acggtgtgac gttctctgtt ccggtcacac
     1321 cacatacgtt ccgccattcc tatgcgatgc acatgctgta tgccggtata ccgctgaaag
     1381 ttctgcaaag cctgatggga cataagtcca tcagttcaac ggaagtctac acgaaggttt
     1441 ttgcgctgga tgtggctgcc cggcaccggg tgcagtttgc gatgccggag tctgatgcgg
     1501 ttgcgatgct gaaacaatta tcctgagaat aaatgccttg gcctttatat ggaaatgtgg
     1561 aactgagtgg atatgctgtt tttgtctgtt aaacagagaa gctggctgtt atccactgag
     1621 aagcgaacga aacagtcggg aaaatctccc attatcgtag agatccgcat tattaatctc
     1681 aggagcctgt gtagcgttta taggaagtag tgttctgtca tgatgcctgc aagcggtaac
     1741 gaaaacgatt tgaatatgcc ttcaggaaca atagaaatct tcgtgcggtg ttacgttgaa
     1801 gtggagcgga ttatgtcagc aatggacaga acaacctaat gaacacagaa ccatgatgtg
     1861 gtctgtcctt ttacagccag taggctcgcc gcagtcgagc gacggcgaag ccctcgagtg
     1921 agcgaggaag caccagggaa cagcacttat atattctgct tacacacgat gcctgaaaaa
     1981 acttcccttg gggttatcca cttatccacg gggatatttt tataattatt ttttttatag
     2041 tttttagatc ttctttttta gagcgccttg taggccttta tccatgctgg ttctagagaa
     2101 ggtgttgtga caaattgccc tttcagtgtg acaaatcacc ctcaaatgac agtcctgtct
     2161 gtgacaaatt gcccttaacc ctgtgacaaa ttgccctcag aagaagctgt tttttcacaa
     2221 agttatccct gcttattgac tcttttttat ttagtgtgac aatctaaaaa cttgtcacac
     2281 ttcacatgga tctgtcatgg cggaaacagc ggttatcaat cacaagaaac gtaaaaatag
     2341 cccgcgaatc gtccagtcaa acgacctcac tgaggcggca tatagtctct cccgggatca
     2401 aaaacgtatg ctgtatctgt tcgttgacca gatcagaaaa tctgatggca ccctacagga
     2461 acatgacggt atctgcgaga tccatgttgc taaatatgct gaaatattcg gattgacctc
     2521 tgcggaagcc agtaaggata tacggcaggc attgaagagt ttcgcgggga aggaagtggt
     2581 tttttatcgc cctgaagagg atgccggcga tgaaaaaggc tatgaatctt ttccttggtt
     2641 tatcaaacgt gcgcacagtc catccagagg gctttacagt gtacatatca acccatatct
     2701 cattcccttc tttatcgggt tacagaaccg gtttacgcag tttcggctta gtgaaacaaa
     2761 agaaatcacc aatccgtatg ccatgcgttt atacgaatcc ctgtgtcagt atcgtaagcc
     2821 ggatggctca ggcatcgtct ctctgaaaat cgactggatc atagagcgtt accagctgcc
     2881 tcaaagttac cagcgtatgc ctgacttccg ccgccgcttc ctgcaggtct gtgttaatga
     2941 gatcaacagc agaactccaa tgcgcctctc atacattgag aaaaagaaag gccgccagac
     3001 gactcatatc gtattttcct tccgcgatat cacttccatg acgacaggat agtctgaggg
     3061 ttatctgtca cagatttgag ggtggttcgt cacatttgtt ctgacctact gagggtaatt
     3121 tgtcacagtt ttgctgtttc cttcagcctg catggatttt ctcatacttt ttgaactgta
     3181 atttttaagg aagccaaatt tgagggcagt ttgtcacagt tgatttcctt ctctttccct
     3241 tcgtcatgtg acctgatatc gggggttagt tcgtcatcat tgatgagggt tgattatcac
     3301 agtttattac tctgaattgg ctatccgcgt gtgtacctct acctggagtt tttcccacgg
     3361 tggatatttc ttcttgcgct gagcgtaaga gctatctgac agaacagttc ttctttgctt
     3421 cctcgccagt tcgctcgcta tgctcggtta cacggctgcg gcgagcgcta gtgataataa
     3481 gtgactgagg tatgtgctcc tcttatctcc ttttgtagtg ttgctcttat tttaaacaac
     3541 tttgcggttt tttgatgact ttgcgatttt gttgttgctt tgcagtaaat tgcaagattt
     3601 aataaaaaaa cgcaaagcaa tgattaaagg atgttcagaa tgaaactcat ggaaacactt
     3661 aaccagtgca taaacgctgg tcatgaaatg acgaaggcta tcgccattgc acagtttaat
     3721 gatgacagcc cggaagcgag gaaaataacc cggcgctgga gaataggtga agcagcggat
     3781 ttagttgggg tttcttctca ggctatcaga gatgccgaga aagcagggcg actaccgcac
     3841 ccggatatgg aaattcgagg acgggttgag caacgagttg gttatacaat tgaacaaatt
     3901 aatcatatgc gtgatgtgtt tggtacgcga ttgcgacgtg ctgaagacgt atttccaccg
     3961 gtgatcgggg ttgctgctca taaaggtggc gtttacaaaa cctcagtttc tgttcatctt
     4021 gctcaggatc tggctctgaa ggggctacga gttttgctcg tggaaggtaa cgacccccag
     4081 ggaacagcct caatgtatca cggatgggta ccagatcttc atattcatgc agaagacact
     4141 ctcctgcctt tctatcttgg ggaaaaggac gatgtcactt atgcaataaa gcccacttgc
     4201 tggccggggc ttgacattat tccttcctgt ctggctctgc accgtattga aactgagtta
     4261 atgggcaaat ttgatgaagg taaactgccc accgatccac acctgatgct ccgactggcc
     4321 attgaaactg ttgctcatga ctatgatgtc atagttattg acagcgcacc taacctgggt
     4381 atcggcacga ttaatgtcgt atgtgctgct gatgtgctga ttgttcccac gcctgctgag
     4441 ttgtttgact acacctccgc actgcagttt ttcgatatgc ttcgtgatct gctcaagaac
     4501 gttgatctta aagggttcga gcctgatgta cgtattttgc ttaccaaata cagcaatagt
     4561 aatggctctc agtccccgtg gatggaggag caaattcggg atgcctgggg aagcatggtt
     4621 ctaaaaaatg ttgtacgtga aacggatgaa gttggtaaag gtcagatccg gatgagaact
     4681 gtttttgaac aggccattga tcaacgctcc tcaactggtg cctggagaaa tgctctttct
     4741 atttgggaac ctgtctgcaa tgaaattttc gatcgtctga ttaaaccacg ctgggagatt
     4801 agataatgaa gcgtgcgcct gttattccaa aacatacgct caatactcaa ccggttgaag
     4861 atacttcgtt atcgacacca gctgccccga tggtggattc gttaattgcg cgcgtaggag
     4921 taatggctcg cggtaatgcc attactttgc ctgtatgtgg tcgggatgtg aagtttactc
     4981 ttgaagtgct ccggggtgat agtgttgaga agacctctcg ggtatggtca ggtaatgaac
     5041 gtgaccagga gctgcttact gaggacgcac tggatgatct catcccttct tttctactga
     5101 ctggtcaaca gacaccggcg ttcggtcgaa gagtatctgg tgtcatagaa attgccgatg
     5161 ggagtcgccg tcgtaaagct gctgcactta ccgaaagtga ttatcgtgtt ctggttggcg
     5221 agctggatga tgagcagatg gctgcattat ccagattggg taacgattat cgcccaacaa
     5281 gtgcttatga acgtggtcag cgttatgcaa gccgattgca gaatgaattt gctggaaata
     5341 tttctgcgct ggctgatgcg gaaaatattt cacgtaagat tattacccgc tgtatcaaca
     5401 ccgccaaatt gcctaaatca gttgttgctc ttttttctca ccccggtgaa ctatctgccc
     5461 ggtcaggtga tgcacttcaa aaagccttta cagataaaga ggaattactt aagcagcagg
     5521 catctaacct tcatgagcag aaaaaagctg gggtgatatt tgaagctgaa gaagttatca
     5581 ctcttttaac ttctgtgctt aaaacgtcat ctgcatcaag aactagttta agctcacgac
     5641 atcagtttgc tcctggagcg acagtattgt ataagggcga taaaatggtg cttaacctgg
     5701 acaggtctcg tgttccaact gagtgtatag agaaaattga ggccattctt aaggaacttg
     5761 aaaagccagc accctgatgc gaccacgttt tagtctacgt ttatctgtct ttacttaatg
     5821 tcctttgtta caggccagaa agcataactg gcctgaatat tctctctggg cccactgttc
     5881 cacttgtatc gtcggtctga taatcagact gggaccacgg tcccactcgt atcgtcggtc
     5941 tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
     6001 ccacggtccc actcgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta
     6061 tcgtcggtct gattattagt ctgggaccat ggtcccactc gtatcgtcgg tctgattatt
     6121 agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg aaccacggtc
     6181 ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt
     6241 ctgattatta gtctgggacc acgatcccac tcgtgttgtc ggtctgatta tcggtctggg
     6301 accacggtcc cacttgtatt gtcgatcaga ctatcagcgt gagactacga ttccatcaat
     6361 gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt agaacggagt aacctcggtg
     6421 tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc ttatccacaa cattttgcgc
     6481 acggttatgt ggacaaaata cctggttacc caggccgtgc cggcacgtta accgggctgc
     6541 atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct cggacatgag gttgccccgt
     6601 attcagtgtc gctgatttgt attgtctgaa gttgttttta cgttaagttg atgcagatca
     6661 attaatacga tacctgcgtc ataattgatt atttgacgtg gtttgatggc ctccacgcac
     6721 gttgtgatat gtagatgata atcattatca ctttacgggt cctttccggt gatccgacag
     6781 gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg gtttaaggcg
     6841 tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct gaaaagaaag
     6901 gaaacgacag gtgctgaaag cgaggctttt tggcctctgt cgtttccttt ctctgttttt
     6961 gtccgtggaa tgaacaatgg aagtcctggc gtaatagcga agaggcccgc accgatcgcc
     7021 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     7081 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     7141 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7201 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7261 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8881 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     8941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaggagcgcc
     9301 caatacgcaa accgcctctg tttaaacgtt ggccgattca ttaatgcagc ttgcgccgtt
     9361 ccacttcgat gcgtcagtga agcgacatga ggcgcgcccg tattcagtgt cgctgatttg
     9421 tattgtctga agttgttttt acgttaagtt gaagcagatc aattaatacg atacctgcgt
     9481 cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagaagat
     9541 aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc
     9601 gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca
     9661 taacttaatg aatttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa
     9721 gcgagctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     9781 aagtccgagc tcatcgctaa taacttcgta tagcatacat tatacgaagt tatattcgat
     9841 gcggccgc
//
