LOCUS       sequence-173863-        9619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of Clover.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9619)
  TITLE     See lab plasmids
REFERENCE   2  (bases 1 to 9619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLNT-SFFV-Clover
FEATURES             Location/Qualifiers
     source          1..9619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(9..11,12..14,15..725)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    766..1354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(819..839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1237..1248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1357..1373)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1358..1374)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1464..1697
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1722..1740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1723..1740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2030..2049)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2263..2282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2382..2404
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2442..2460)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2528..2632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2633..2701,2702..3493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2851..2870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3664..4252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4153..4172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4406..4423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4493..4513)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4498..4827
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4678..4813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5961..6026
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6156..6176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6601..6735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6638..6657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6692..6711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6903..7536
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7583..7708
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8201..8434
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8619..8663
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8948..9065
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9218..9619
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 gatccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc gagggcgatg
      121 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ttcggctacg gcgtggcctg cttcagccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcacg gccgacaagc
      481 agaagaacgg catcaaggct aacttcaaga tccgccacaa cgttgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagccat cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggattac acatggcatg gacgagctgt
      721 acaagtaacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
      781 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
      841 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
      901 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
      961 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     1021 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     1081 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     1141 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     1201 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     1261 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     1321 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     1381 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     1441 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     1501 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1561 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1621 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1681 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     1741 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     1801 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     1861 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     1921 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     1981 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     2041 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     2101 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     2161 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     2221 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     2281 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2341 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2401 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2461 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4441 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     4501 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4561 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4621 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4681 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4741 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4801 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     4861 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     4921 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     4981 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     5041 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     5101 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     5161 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     5221 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     5281 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     5341 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     5401 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     5461 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     5521 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     5581 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     5641 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     5701 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     5761 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     5821 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     5881 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     5941 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     6001 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     6061 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     6121 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     6181 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     6241 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     6301 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     6361 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     6421 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     6481 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     6541 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     6601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6721 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     6781 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     6841 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     6901 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     6961 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     7021 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     7081 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     7141 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     7201 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     7261 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     7321 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     7381 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7441 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7501 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7561 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7621 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7681 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7741 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7801 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7861 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7921 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7981 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8041 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8101 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8161 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8221 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8281 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8341 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8401 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8461 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8521 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8581 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8641 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8701 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8761 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8821 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8881 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca
     8941 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9001 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9061 attttcgggt ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg
     9121 aattcctgca gccccgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     9181 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
     9241 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     9301 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     9361 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     9421 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     9481 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     9541 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     9601 cctccgacag actgagtcg
//
