LOCUS       sequence-173864-        9713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  TITLE     See lab plasmids
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLNT-SFFV-mRuby2
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..620
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(85..105)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(503..514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(623..639)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(624..640)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             730..963
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(988..1006)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(989..1006)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1296..1315)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1529..1548)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1648..1670
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1708..1726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1794..1898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1899..1967,1968..2759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2117..2136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3672..3689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3759..3779)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3764..4093
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3944..4079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5227..5292
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5422..5442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5867..6001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5904..5923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5958..5977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6169..6802
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6849..6974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7467..7700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7885..7929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8214..8331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8484..8885
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8909..8926
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8930..8962
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(8966..8974,8975..8989)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             8996..9706
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
       61 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      121 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      181 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      241 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
      301 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
      361 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
      421 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
      481 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
      541 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
      601 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
      661 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
      721 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
      781 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      841 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      901 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      961 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     1021 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     1081 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     1141 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     1201 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     1261 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1321 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1381 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     1441 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1501 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     1561 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1621 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1681 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1741 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3721 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     3781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3841 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3901 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4021 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4081 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     4141 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     4201 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     4261 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     4321 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     4381 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     4441 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     4501 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     4561 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     4621 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     4681 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     4741 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     4801 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     4861 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     4921 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     4981 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     5041 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     5101 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     5161 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     5221 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     5281 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     5341 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     5401 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     5461 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     5521 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     5581 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     5641 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     5701 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     5761 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6001 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     6061 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     6121 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     6181 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     6241 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     6301 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     6361 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     6421 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     6481 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     6541 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     6601 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6661 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6721 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6781 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     6841 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     6901 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     6961 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     7021 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     7081 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     7141 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     7201 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     7261 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     7321 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     7381 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     7441 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     7501 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     7561 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     7621 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     7681 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     7741 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     7801 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     7861 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     7921 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     7981 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     8041 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     8101 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     8161 cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa
     8221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     8281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     8341 tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt cctgcagccc
     8401 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     8461 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     8521 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     8581 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     8641 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     8701 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     8761 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     8821 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg
     8881 agtcggatcc accatggtgc ggggttctca tcatcatcat catcatggta tggctagcat
     8941 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgatggt
     9001 gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc
     9061 ggtcaacggc caccaattca aatgcacagg tgaaggagaa ggcaatccgt acatgggaac
     9121 tcaaaccatg aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct
     9181 tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag tacccgaaag gcattcctga
     9241 tttctttaaa cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga
     9301 tggtggagtc gtcaccgtca tgcaggacac cagccttgag gatggctgtc tcgtttacca
     9361 cgtccaagtc agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa
     9421 gggttgggag cctaatacag agatgatgta tccagcagat ggtggtctga ggggatacac
     9481 tcatatggca ctgaaagttg atggtggtgg ccatctgtct tgctctttcg taacaactta
     9541 caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt atccatgccg ttgatcaccg
     9601 cctggaaagg ttagaggaaa gtgacaatga aatgttcgta gtacaacgcg aacacgcagt
     9661 tgccaagttc gccgggcttg gtggtgggat ggacgagctg tacaagtaac gca
//
