LOCUS       sequence-173868-        8215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral stable expression of chimeric protein TRF2ZNF1-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pLPC-Myc-TRF2ZNF1-11
FEATURES             Location/Qualifiers
     source          1..8215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(129..158)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(198..217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(199..217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(262..465)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(291..311)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        466..845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1017..1036
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1125..1724)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1208..1228)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1705..1724
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(1742..2158)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1758..1774)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1790..1812)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2359..2558
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             2623..3092
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3123..3227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3228..3296,3297..4088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3446..3465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4248..4836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4737..4756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4990..5007
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             5137..5606
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gggcccccca gccccggctc gtggcgcccc cgccgggccc gcccgctact gcgggacgcc
       61 cgcctgccag ctgcccgccc gctgccgtcg ctactcccgc ctcctcccgc ggatcctctc
      121 tttcttaaca aatcttcttc agaaatcaac ttttgttcca tggtggctga tccgagctcg
      181 gtaccaagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
      241 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
      301 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
      361 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
      421 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
      481 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
      541 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
      601 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
      661 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
      721 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
      781 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
      841 atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag
      901 cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc
      961 gcactactca gcgacctcca acacacaagc agggagcaga tactggctta actatgcggc
     1021 atcagagcag attgtactga gagtgcacca taggggatcg ggagatccga tgcatggggt
     1081 cgtgcgctcc tttcggtcgg gcgctgcggg tcgtggggcg ggcgtcaggc accgggcttg
     1141 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
     1201 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
     1261 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
     1321 tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
     1381 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
     1441 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
     1501 accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
     1561 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg
     1621 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg
     1681 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggtaag ctgatccggc
     1741 cggcgcctag agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag
     1801 gaggttcaag ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta
     1861 caaagggctt gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg
     1921 ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga
     1981 tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg
     2041 cagagcagaa ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc
     2101 gctcgacatc tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggccct
     2161 aattttcaga caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc
     2221 gctgcagcag acaagacgcg cggcttcggt tccaaaccga aagcaaaaat tcagacggag
     2281 gcgggaactg ttttaggttc tcgtctccta ccagaaccac atatcctgac ggggtcggat
     2341 tccacatcga ctcccttcct caggtcgggc cacaaaaacg gcccccaaag tccctgggac
     2401 gtctcccagg gttgcggccg ggtgttcaga actcgtcagt tccaccacgg gtccgccaga
     2461 tacagagcta gttagctaac tagtaccgac gcaggcgcat aaaatcagtc atagacacta
     2521 gacaatcgga cagacacaga taagttgctg gccagcttgc ctcccggtgg tgggtcggtg
     2581 gtccctgggc aggggtctcc cgatcccgga cgagccccca aatgaaagac ccccgctgac
     2641 gggtagtcaa tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc
     2701 aactgcaaga gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact
     2761 ggcgccccga gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga
     2821 acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc
     2881 cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat
     2941 ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     3001 gtttggccca tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct
     3061 taccacagat atcctgtttg gcccatattc aggctgcagg tggcactttt cggggaaatg
     3121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3181 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3241 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3301 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3361 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3421 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3481 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3541 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3601 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3661 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3721 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3781 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3841 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3901 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3961 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4021 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4081 attggtaact gtcagaccaa gtttactcat atatacttta gattgatttg cggccgcaaa
     4141 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4201 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4261 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4321 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4381 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4441 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4501 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4561 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4621 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4681 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4741 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4801 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4861 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4921 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4981 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5041 ctccccgcgc gttggccgat tcattaatgc aactatggcc atttaatgta aatacttaag
     5101 aaaaaaaacc aaattaattt tgatacatgc tgcatgtgaa agacccccgc tgacgggtag
     5161 tcaatcactc agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc
     5221 aagagggttt attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc
     5281 ccgagtgagg ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa
     5341 gcgagaagcg aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg
     5401 gcaccctgga aacatctgat ggttctctag aaactgctga gggctggacc gcatctgggg
     5461 accatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     5521 cccatattca gctgttccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac
     5581 agatatcctg tttggcccat attcagctgt tccatctgtt cctgaccttg atctgaactt
     5641 ctctattctc agttatgtat ttttccatgc cttgcaaaat ggcgttactt aagctagctt
     5701 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
     5761 ttattttatc ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
     5821 gaattcctat gtgtcacagt cctcggggac agcagctgtg atgatgaggg agggctccag
     5881 gacacctggg ccggtgaagc tctcctctgc acacagtctc tggccgggga gctgggaggc
     5941 caggccgccc tgggccaggg actccacaat gacctccgcc gagcccacct ccagctctgc
     6001 aggcggctgc agcgccacca ccaccatctt ctcgtcctcg atgatcaggt tgcggagctt
     6061 gcgctgccgc gggttgtagt tctctacccg gtcgtggatc tccatgtgcc tccgcaggtg
     6121 ggcctgtcgg gtaaacttgt agccacactc ggtgcactca aacttcctca cccccttgtg
     6181 ccgtctgacg tgcacacgca gttgctccac ggctttgaag gtcttgccgc atatctggca
     6241 gaagtggggc cggccctcct gatggcggct ggccacgtgc ctcaggaggg gccccttctc
     6301 agtgaagcgc tgttcacaga actcgcaact gaagggcctt tccccggtgt gggtgcggtt
     6361 gtgcttgtcc aggctggctt gggttcggaa ggtcttgcca cagaggtggc actggaaggg
     6421 cttctcaccc gtgtgtgtgc gcaggtgcat gttgagattg gccttttggg tgaaggcgtg
     6481 gctgcagaac tcacatacgt gtggcctctc attcctgtgc ttggccttga tgtgcatctg
     6541 caggccattc cggctcgagg cccggtgccc acactcctca cacacaaaca gcttctcacc
     6601 acggtgcttg aaagcttcat gcagctgcag ctctgtccga gacaggaagc acttggcaca
     6661 ggtggggcat gcatggggct tgggggctcc atgaagtttg atcatgtggc tctgcaagtc
     6721 cttcttctgc atgaactgct gggagcagga ggaacacttg tagggcatct cccctgtgtg
     6781 agacaccatg tgcacccgca gctccatcct tcggcggaat gtctcctggc acacagagca
     6841 cgtgaagacc tgttccgagc ggttcatgca attccgggct tcatgctcca ggaggttctc
     6901 cttccgaaag taacacttcc cacatttggg acactcaaag ggtttctccc cagtatgttt
     6961 cctcatggat ccttctacag tccacttctg cttttttgtt atattggttg tactgtcttc
     7021 atctggtgcc tgaacttgaa acagttcatc ctcttccacc caagtctcct tttcttcaac
     7081 cccattagag ctgttccact tgcctttggg tactttggga ttcttctctc cagggagggg
     7141 ttggttgaga acggtgggct tggatggtgg cgctgaagcg gcctcctggg aggagttgag
     7201 gcctgcgctg ggctcagtac tctggctgtc ctcctccaag accaatctgc ttattgtcat
     7261 gcgcttgttc ttgggctgca gttccgagcc accctcaccg tcagccgggg ctgaactttc
     7321 gttttcatct tttctgggtc tcttgttttt gagggctggt gatgctggga gagcttgagt
     7381 aggaagaacc agatccttct ggtccagttt tgcaaaggct gcctcagaat cctgtgcacc
     7441 agacagagtc ttgaaagctg ctttcagagt catcattcca atggtggttg gaggattccg
     7501 tagctgcctt gcgggttctc tgggtggctt ttccacaggc cctggtgctg gctgtttatc
     7561 ttccttccct gtacttgagg cagcggactc agatttcaaa gcctttttgg ccatcgtgag
     7621 gaggtagggc tcggcgtcat ccaggtggct ctccaggaag cgcagcatct tctgctggaa
     7681 ggtctcatat gaaaagttct ggataacagg atgggccaag ttcttttctc gaataatatt
     7741 caggagatca tttctcagct tctgagttgt ggggtccttg gacatatgtt ttttcaaaat
     7801 ttttgaagcc ttttcaaatt ctttgttttt gatacaaata atgacagcag cttccttgac
     7861 cagttttcta ctggattcga ccactgcttc tgtcagtgta aattccgttt taatcatctc
     7921 cagcacattg atagctgatt ccagtggtgt gagctcagcc tccatatcaa aggaacagtc
     7981 taaattttcc ccttcttcaa tccgcgacag acactgcata acccgcagca atcgggacac
     8041 ggtgtgctcc ttccccaagg gcctgacaag caaagcctgc atgatgtccc ggatctgtct
     8101 gaagtccccg taccggctac cccgaaaggc ccgcagcgcc tcgtggaagt agaacttgag
     8161 cacccagcga ttgactgcct cttccagccg tgcctccccc gcgccgcgct ccgcc
//
