LOCUS       sequence-173871-        7714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral stable expression of chimeric protein TRF2ZNF1-8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pLPC-Myc-TRF2ZNF1-8
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2168..2637
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2767..2784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2938..3526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3018..3037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3686..4477,4478..4546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4309..4328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4547..4651)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4682..5151
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5216..5415
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5616..6032
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5962..5984
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6000..6016
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6050..6649
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6050..6069)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6546..6566
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6738..6757)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6929..7308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7309..7512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7463..7483
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7557..7576
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7557..7575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7607..7616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7616..7645
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcgtcccgca gtagcgggcg ggcccggcgg gggcgccacg agccggggct ggggggcccg
       61 gcggagcgcg gcgcggggga ggcacggctg gaagaggcag tcaatcgctg ggtgctcaag
      121 ttctacttcc acgaggcgct gcgggccttt cggggtagcc ggtacgggga cttcagacag
      181 atccgggaca tcatgcaggc tttgcttgtc aggcccttgg ggaaggagca caccgtgtcc
      241 cgattgctgc gggttatgca gtgtctgtcg cggattgaag aaggggaaaa tttagactgt
      301 tcctttgata tggaggctga gctcacacca ctggaatcag ctatcaatgt gctggagatg
      361 attaaaacgg aatttacact gacagaagca gtggtcgaat ccagtagaaa actggtcaag
      421 gaagctgctg tcattatttg tatcaaaaac aaagaatttg aaaaggcttc aaaaattttg
      481 aaaaaacata tgtccaagga ccccacaact cagaagctga gaaatgatct cctgaatatt
      541 attcgagaaa agaacttggc ccatcctgtt atccagaact tttcatatga gaccttccag
      601 cagaagatgc tgcgcttcct ggagagccac ctggatgacg ccgagcccta cctcctcacg
      661 atggccaaaa aggctttgaa atctgagtcc gctgcctcaa gtacagggaa ggaagataaa
      721 cagccagcac cagggcctgt ggaaaagcca cccagagaac ccgcaaggca gctacggaat
      781 cctccaacca ccattggaat gatgactctg aaagcagctt tcaagactct gtctggtgca
      841 caggattctg aggcagcctt tgcaaaactg gaccagaagg atctggttct tcctactcaa
      901 gctctcccag catcaccagc cctcaaaaac aagagaccca gaaaagatga aaacgaaagt
      961 tcagccccgg ctgacggtga gggtggctcg gaactgcagc ccaagaacaa gcgcatgaca
     1021 ataagcagat tggtcttgga ggaggacagc cagagtactg agcccagcgc aggcctcaac
     1081 tcctcccagg aggccgcttc agcgccacca tccaagccca ccgttctcaa ccaacccctc
     1141 cctggagaga agaatcccaa agtacccaaa ggcaagtgga acagctctaa tggggttgaa
     1201 gaaaaggaga cttgggtgga agaggatgaa ctgtttcaag ttcaggcacc agatgaagac
     1261 agtacaacca atataacaaa aaagcagaag tggactgtag aaggatccat gaggaaacat
     1321 actggggaga aaccctttga gtgtcccaaa tgtgggaagt gttactttcg gaaggagaac
     1381 ctcctggagc atgaagcccg gaattgcatg aaccgctcgg aacaggtctt cacgtgctct
     1441 gtgtgccagg agacattccg ccgaaggatg gagctgcggg tgcacatggt gtctcacaca
     1501 ggggagatgc cctacaagtg ttcctcctgc tcccagcagt tcatgcagaa gaaggacttg
     1561 cagagccaca tgatcaaact tcatggagcc cccaagcccc atgcatgccc cacctgtgcc
     1621 aagtgcttcc tgtctcggac agagctgcag ctgcatgaag ctttcaagca ccgtggtgag
     1681 aagctgtttg tgtgtgagga gtgtgggcac cgggcctcga gccggaatgg cctgcagatg
     1741 cacatcaagg ccaagcacag gaatgagagg ccacacgtat gtgagttctg cagccacgcc
     1801 ttcacccaaa aggccaatct caacatgcac ctgcgcacac acacgggtga gaagcccttc
     1861 cagtgccacc tctgtggcaa gaccttccga acccaagcca gcctggacaa gcacaaccgc
     1921 acccacaccg gggaaaggcc cttctaggaa ttctgcagat atccatcaca ctggcggccg
     1981 ctcgagcatg catctagagg gccgataaaa taaaagattt tatttagtct ccagaaaaag
     2041 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     2101 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     2161 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     2221 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     2281 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     2341 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     2401 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     2461 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     2521 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     2581 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat
     2641 gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata
     2701 gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3601 gagattatca aaaaggatct tcacctagat ccttttgcgg ccgcaaatca atctaaagta
     3661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcag
     4681 cctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     4741 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4801 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     4861 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     4921 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     4981 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     5041 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     5101 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc
     5161 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggcaagct
     5221 ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc
     5281 tgcgtcggta ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg
     5341 agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt
     5401 gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc
     5461 tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
     5521 ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct
     5581 gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa
     5641 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     5701 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     5761 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     5821 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     5881 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     5941 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     6001 tttatccagc cctcactcct tctctaggcg ccggccggat cagcttacca tgaccgagta
     6061 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     6121 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6181 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6241 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6301 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6361 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6421 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6481 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6541 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6601 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     6661 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcggatc tcccgatccc
     6721 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     6781 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     6841 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6901 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     6961 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     7021 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     7081 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     7141 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     7201 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     7261 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     7321 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     7381 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     7441 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     7501 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     7561 acgactcact atagggagac ccaagcttgg taccgagctc ggatcagcca ccatggaaca
     7621 aaagttgatt tctgaagaag atttgttaag aaagagagga tccgcgggag gaggcgggag
     7681 tagcgacggc agcgggcggg cagctggcag gcgg
//
