LOCUS       sequence-173877-        7114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral stable expression of truncation protein Flag-TZAPZNF7-9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pLPC-Flag-TZAPZNF7-9
FEATURES             Location/Qualifiers
     source          1..7114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             435..1034
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(435..454)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     931..951
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1123..1142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1314..1693
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1694..1897
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1848..1868
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1942..1961
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1942..1960
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1992..2001
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2001..2024
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             3667..4136
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4266..4283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4437..5025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4517..4536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5185..5976,5977..6045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5808..5827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6046..6150)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6181..6650
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6715..6914
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatcagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      481 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      541 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      601 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      661 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      721 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      781 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      841 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
      901 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
      961 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     1021 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     1081 cccatgcatc ggatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     1141 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     1201 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     1261 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     1321 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     1381 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     1441 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     1501 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1561 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1621 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     1681 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1741 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1801 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1861 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1921 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg
     1981 agctcggatc agccaccatg gactacaaag acgatgacga taaaggatcc atggacggct
     2041 ccttcgtcca gcacagtgtg agggttctgc aggagctcaa caagcagcgg gagaagggcc
     2101 agtactgcga cgccactctg gacgtggggg gcctggtgtt taaggcacac tggagtgtcc
     2161 ttgcctgctg cagtcacttt ttccagagcc tctacgggga tggctcaggg ggcagtgtcg
     2221 tcctccctgc tggcttcgct gagatctttg gcctcttgtt ggactttttc tacactggtc
     2281 acctcgctct cacctcaggg aaccgggatc aggtgctcct ggcagccagg gagttgcgag
     2341 tgccagaggc cgtagagctg tgccagagct tcaagcccaa aacttcagtg ggacaggcag
     2401 caggtggcca gagtgggctg gggccccctg cctcccagaa tgtgaacagc cacgtcaagg
     2461 agccggcagg cttggaagaa gaggaagttt cgaggactct gggtctagtc cccagggatc
     2521 aggagcccag aggcagtcat agtcctcaga ggccccagct ccattcccca gctcagagtg
     2581 agggcccctc ctccctctgt gggaaactga agcaggcctt gaagccttgt ccccttgagg
     2641 acaagaaacc cgaggactgc aaagtgcccc caaggccctt agaggctgaa ggtgcccagc
     2701 tgcagggcgg cagtaatgag tgggaagtgg tggttcaagt ggaggatgat ggggatggcg
     2761 attacatgtc tgagcctgag gctgtgctga ccaggaggaa gtcaaatgta atccgaaagc
     2821 cctgtgcagc tgagccagcc ctgagcgcgg gctccctagc agctgagcct gctgagaaca
     2881 gaaaaggtac agcggtgccg gtcgaaaatg agaggccaca cgtatgtgag ttctgcagcc
     2941 acgccttcac ccaaaaggcc aatctcaaca tgcacctgcg cacacacacg ggtgagaagc
     3001 ccttccagtg ccacctctgt ggcaagacct tccgaaccca agccagcctg gacaagcaca
     3061 accgcaccca caccggggaa aggcccttca gttgcgagtt ctgtgaacag cgcttcactg
     3121 agaaggggcc cctcctgagg cacgtggcca gccgccatca ggagggccgg ccccacttcg
     3181 accgggtaga gaactacaac ccgcggcagc gcaagctccg caacctgatc atcgaggacg
     3241 agaagatggt ggtggtggcg ctgcagccgc ctgcagagct ggaggtgggc tcggcggagg
     3301 tcattgtgga gtccctggcc cagggcggcc tggcctccca gctccccggc cagagactgt
     3361 gtgcagagga gagcttcacc ggcccaggtg tcctggagcc ctccctcatc atcacagctg
     3421 ctgtccccga ggactgtgac acataggaat tctgcagata tccatcacac tggcggccgc
     3481 tcgagcatgc atctagaggg ccgataaaat aaaagatttt atttagtctc cagaaaaagg
     3541 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     3601 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     3661 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     3721 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     3781 cctgccccga ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     3841 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     3901 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     3961 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     4021 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     4081 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     4141 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
     4201 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4861 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5101 agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat
     5161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcagc
     6181 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     6241 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     6301 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     6361 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     6421 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     6481 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     6541 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     6601 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     6661 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
     6721 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     6781 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     6841 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     6901 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     6961 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     7021 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
     7081 tctgactgtg tttctgtatt tgtctgaaaa ttag
//
