LOCUS       sequence-173878-        7096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral stable expression of truncation protein Flag-TZAPZNF9-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pLPC-Flag-TZAPZNF9-11
FEATURES             Location/Qualifiers
     source          1..7096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..471
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(601..618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(772..1360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(852..871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1520..2311,2312..2380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2143..2162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2381..2485)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2516..2985
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3050..3249
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3450..3866
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3796..3818
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3834..3850
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3884..4483
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3884..3903)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4380..4400
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4572..4591)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4763..5142
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5143..5346
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5297..5317
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5391..5410
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5391..5409
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5441..5450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5450..5473
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
       61 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
      121 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
      181 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
      241 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
      301 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
      361 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
      421 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
      481 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
      541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1441 atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa agtatatatg
     1501 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1561 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1621 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1681 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1741 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1801 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1861 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1921 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1981 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2041 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2101 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2161 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2221 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2281 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2341 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2401 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2461 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcagcctgaa
     2521 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     2581 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     2641 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     2701 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     2761 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     2821 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     2881 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     2941 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     3001 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca agctggccag
     3061 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
     3121 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     3181 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     3241 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     3301 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     3361 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     3421 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     3481 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     3541 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     3601 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     3661 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     3721 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     3781 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     3841 cagccctcac tccttctcta ggcgccggcc ggatcagctt accatgaccg agtacaagcc
     3901 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3961 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4021 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4081 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4141 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4201 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4261 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4321 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4381 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4441 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     4501 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gatctcccga tcccctatgg
     4561 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     4621 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     4681 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     4741 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     4801 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4861 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4921 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4981 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     5041 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     5101 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     5161 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5221 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     5281 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5341 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     5401 cactataggg agacccaagc ttggtaccga gctcggatca gccaccatgg actacaaaga
     5461 cgatgacgat aaaggatcca tggacggctc cttcgtccag cacagtgtga gggttctgca
     5521 ggagctcaac aagcagcggg agaagggcca gtactgcgac gccactctgg acgtgggggg
     5581 cctggtgttt aaggcacact ggagtgtcct tgcctgctgc agtcactttt tccagagcct
     5641 ctacggggat ggctcagggg gcagtgtcgt cctccctgct ggcttcgctg agatctttgg
     5701 cctcttgttg gactttttct acactggtca cctcgctctc acctcaggga accgggatca
     5761 ggtgctcctg gcagccaggg agttgcgagt gccagaggcc gtagagctgt gccagagctt
     5821 caagcccaaa acttcagtgg gacaggcagc aggtggccag agtgggctgg ggccccctgc
     5881 ctcccagaat gtgaacagcc acgtcaagga gccggcaggc ttggaagaag aggaagtttc
     5941 gaggactctg ggtctagtcc ccagggatca ggagcccaga ggcagtcata gtcctcagag
     6001 gccccagctc cattccccag ctcagagtga gggcccctcc tccctctgtg ggaaactgaa
     6061 gcaggccttg aagccttgtc cccttgagga caagaaaccc gaggactgca aagtgccccc
     6121 aaggccctta gaggctgaag gtgcccagct gcagggcggc agtaatgagt gggaagtggt
     6181 ggttcaagtg gaggatgatg gggatggcga ttacatgtct gagcctgagg ctgtgctgac
     6241 caggaggaag tcaaatgtaa tccgaaagcc ctgtgcagct gagccagccc tgagcgcggg
     6301 ctccctagca gctgagcctg ctgagaacag aaaaggtaca gcggtgccgg tcgaaaccgg
     6361 ggaaaggccc ttcagttgcg agttctgtga acagcgcttc actgagaagg ggcccctcct
     6421 gaggcacgtg gccagccgcc atcaggaggg ccggccccac ttctgccaga tatgcggcaa
     6481 gaccttcaaa gccgtggagc aactgcgtgt gcacgtcaga cggcacaagg gggtgaggaa
     6541 gtttgagtgc accgagtgtg gctacaagtt tacccgacag gcccacctgc ggaggcacat
     6601 ggagatccac gaccgggtag agaactacaa cccgcggcag cgcaagctcc gcaacctgat
     6661 catcgaggac gagaagatgg tggtggtggc gctgcagccg cctgcagagc tggaggtggg
     6721 ctcggcggag gtcattgtgg agtccctggc ccagggcggc ctggcctccc agctccccgg
     6781 ccagagactg tgtgcagagg agagcttcac cggcccaggt gtcctggagc cctccctcat
     6841 catcacagct gctgtccccg aggactgtga cacataggaa ttctgcagat atccatcaca
     6901 ctggcggccg ctcgagcatg catctagagg gccgataaaa taaaagattt tatttagtct
     6961 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     7021 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     7081 aggaacagat ggaaca
//
