LOCUS       sequence-173880-        7597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral stable expression of chimeric protein TRF2ZNF9-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder
            J, Lazzerini Denchi E
  TITLE     TZAP: A telomere-associated protein involved in telomere length 
            control.
  JOURNAL   Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752.
  PUBMED    28082411
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752."
COMMENT     Sequence Label: pLPC-Myc-TRF2ZNF9-11
FEATURES             Location/Qualifiers
     source          1..7597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2049..2518
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2648..2665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2819..3407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2899..2918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3567..4358,4359..4427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4190..4209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4428..4532)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4563..5032
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5097..5296
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5497..5913
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5843..5865
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5881..5897
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5931..6530
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5931..5950)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6427..6447
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6619..6638)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6810..7189
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7190..7393
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7344..7364
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7438..7457
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7438..7456
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7488..7497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7497..7526
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtcccgcagt agcgggcggg cccggcgggg gcgccacgag ccggggctgg ggggcccggc
       61 ggagcgcggc gcgggggagg cacggctgga agaggcagtc aatcgctggg tgctcaagtt
      121 ctacttccac gaggcgctgc gggcctttcg gggtagccgg tacggggact tcagacagat
      181 ccgggacatc atgcaggctt tgcttgtcag gcccttgggg aaggagcaca ccgtgtcccg
      241 attgctgcgg gttatgcagt gtctgtcgcg gattgaagaa ggggaaaatt tagactgttc
      301 ctttgatatg gaggctgagc tcacaccact ggaatcagct atcaatgtgc tggagatgat
      361 taaaacggaa tttacactga cagaagcagt ggtcgaatcc agtagaaaac tggtcaagga
      421 agctgctgtc attatttgta tcaaaaacaa agaatttgaa aaggcttcaa aaattttgaa
      481 aaaacatatg tccaaggacc ccacaactca gaagctgaga aatgatctcc tgaatattat
      541 tcgagaaaag aacttggccc atcctgttat ccagaacttt tcatatgaga ccttccagca
      601 gaagatgctg cgcttcctgg agagccacct ggatgacgcc gagccctacc tcctcacgat
      661 ggccaaaaag gctttgaaat ctgagtccgc tgcctcaagt acagggaagg aagataaaca
      721 gccagcacca gggcctgtgg aaaagccacc cagagaaccc gcaaggcagc tacggaatcc
      781 tccaaccacc attggaatga tgactctgaa agcagctttc aagactctgt ctggtgcaca
      841 ggattctgag gcagcctttg caaaactgga ccagaaggat ctggttcttc ctactcaagc
      901 tctcccagca tcaccagccc tcaaaaacaa gagacccaga aaagatgaaa acgaaagttc
      961 agccccggct gacggtgagg gtggctcgga actgcagccc aagaacaagc gcatgacaat
     1021 aagcagattg gtcttggagg aggacagcca gagtactgag cccagcgcag gcctcaactc
     1081 ctcccaggag gccgcttcag cgccaccatc caagcccacc gttctcaacc aacccctccc
     1141 tggagagaag aatcccaaag tacccaaagg caagtggaac agctctaatg gggttgaaga
     1201 aaaggagact tgggtggaag aggatgaact gtttcaagtt caggcaccag atgaagacag
     1261 tacaaccaat ataacaaaaa agcagaagtg gactgtagaa ggatccaccg gggaaaggcc
     1321 cttcagttgc gagttctgtg aacagcgctt cactgagaag gggcccctcc tgaggcacgt
     1381 ggccagccgc catcaggagg gccggcccca cttctgccag atatgcggca agaccttcaa
     1441 agccgtggag caactgcgtg tgcacgtcag acggcacaag ggggtgagga agtttgagtg
     1501 caccgagtgt ggctacaagt ttacccgaca ggcccacctg cggaggcaca tggagatcca
     1561 cgaccgggta gagaactaca acccgcggca gcgcaagctc cgcaacctga tcatcgagga
     1621 cgagaagatg gtggtggtgg cgctgcagcc gcctgcagag ctggaggtgg gctcggcgga
     1681 ggtcattgtg gagtccctgg cccagggcgg cctggcctcc cagctccccg gccagagact
     1741 gtgtgcagag gagagcttca ccggcccagg tgtcctggag ccctccctca tcatcacagc
     1801 tgctgtcccc gaggactgtg acacatagga attctgcaga tatccatcac actggcggcc
     1861 gctcgagcat gcatctagag ggccgataaa ataaaagatt ttatttagtc tccagaaaaa
     1921 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     1981 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     2041 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     2101 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     2161 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     2221 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     2281 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     2341 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     2401 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     2461 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     2521 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     2581 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3481 tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccgcaaatc aatctaaagt
     3541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgca
     4561 gcctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     4621 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4681 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     4741 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     4801 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     4861 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     4921 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     4981 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     5041 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc
     5101 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
     5161 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     5221 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     5281 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     5341 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     5401 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     5461 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     5521 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     5581 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     5641 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     5701 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     5761 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     5821 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     5881 ctttatccag ccctcactcc ttctctaggc gccggccgga tcagcttacc atgaccgagt
     5941 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     6001 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     6061 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     6121 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     6181 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     6241 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     6301 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     6361 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     6421 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     6481 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     6541 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcggat ctcccgatcc
     6601 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     6661 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     6721 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     6781 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     6841 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6901 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6961 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     7021 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     7081 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     7141 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     7201 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     7261 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     7321 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     7381 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     7441 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatcagcc accatggaac
     7501 aaaagttgat ttctgaagaa gatttgttaa gaaagagagg atccgcggga ggaggcggga
     7561 gtagcgacgg cagcgggcgg gcagctggca ggcgggc
//
