LOCUS       sequence-173892-        9702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human monocarboxylate transporter 2 (MCT2) promoter 4.2 kbp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, 
            Dave AM, Kiousis S, Sloan AE, Mathupala SP
  TITLE     pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity 
            Monocarboxylate Transporter Expression in Glioblastoma.
  JOURNAL   Neoplasia. 2017 Jan 13;19(2):121-134. doi: 
            10.1016/j.neo.2016.12.011.
  PUBMED    28092823
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2016.12"; journalName: "Neoplasia"; date: 
            "2017-01-13- 13"; volume: "19"; issue: "2"; pages: "121-134"
COMMENT     Sequence Label: pGL2-MCT2Prom4.2
FEATURES             Location/Qualifiers
     source          1..9702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(118..137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     328..349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    676..797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             5002..6654
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(5068..5086)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          6895..6960
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7090..7110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7535..7669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7811..7828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7982..8570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8062..8081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8741..9532,9533..9601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9364..9383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9602..9702,1..4))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
       61 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      301 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      421 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
      481 caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      541 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgtggag
      601 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      661 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      721 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      781 ctcatcaatg tatcttatgg tactgtaact gagctaacat aacccgggag gtaccactag
      841 tcttaaggac acattagtgc actttcaaaa gttcaatgtt tcattgggga ttttatgcct
      901 ttatgattta caatttcttt tttcgagacg gaattttgct cttgctgccc aggctggagt
      961 gcagtgttgc gatcttggct cactgcaacc tctgcctccc ggattcaagc gattcttctg
     1021 cctcagcctc ccgaagtacc tgggattaca ggtgcccacc acctcacctg gctaattttt
     1081 tttttttttt tttgtatttt tagttgagat gggttttcac catgttggct aggctagtct
     1141 tgaacttctg acctcaggtg atctacctgc cttggcctcc caaagtgctg ggattacaag
     1201 catgagccac cgcgcctggc tgacttattg tctttttcag taatatctta tattggtcag
     1261 aataggctag gttttgttcc catgacaaat agagcctgaa atttccaagg cttaaaacaa
     1321 tggtctcttg ctcacacaaa gtacagtgca attcaagcag tctttctttc tttttcagag
     1381 attccatttt gtagctagtt catcagaaac atggtactat ggcagggaaa gggagtgatg
     1441 aaggaggtac actgggtctt aactcccttt accagaaatt acacatgtca cttccattca
     1501 tactgtttta gaactacata gttgcatggt cccaatataa ctgtaaggaa agccaggaag
     1561 tgtggagcca ggcagatcat agatattgtg agcaccaata gcttctacca catatctgtt
     1621 gcctacagcc acactgtcat ctcttttctt catttggcaa acgttactat tttctatctg
     1681 caacgatttg tgctgcatga tagatgattc tgttacccga aaggggtact gattcagacc
     1741 ccaagagagg gttcttggat ttcatgcaag aaataatttg ggatgagtct ataaatgaaa
     1801 gcaggagtac taggaaagta aaggaacaaa aaaatggcta caccataggc agagtagcag
     1861 catgggctgc tccgctgctt atacttatta ttacctcttg attatatgtt aacaagagat
     1921 ggattattca tgagttttct gggaaagggg tgggaaattc tgggaactga gggtttctcc
     1981 ccattttaga ccatatgggg taacttcctg accttaccat ggcatttgta aactgtcatg
     2041 gcactggtgg gaatgtcttt tagcatgcta atgcattgta attagcacat aatgaggagt
     2101 gaggaggacc agaggtcact ttcgtctcca ttttggtttt ggtgggtttt agctggcttc
     2161 tttactgccg cctgttttat cagcatggtc tttatgacct gtatcttgtg ccaacctcct
     2221 atcttattct gtgactaaga atgccttaat ctcctgggaa tgcagcccaa taagtctcag
     2281 cctcatttta cccaggcctt actcaagatg gagtcactct ggttcaaatg cctgtgacaa
     2341 tcccagagat gaataaaatg tcatctctac tttcaaatag cctaatatat tggggctaca
     2401 gaaacaaaaa tcatattata atatagtcat atgtgaagct gaaacaaaat gccccagata
     2461 gaggcctaaa tcattttcta tgggcaggat cacagagaaa gatgaactta acccttattt
     2521 gttaacaaag aatccttaaa atgctataga agtgatgata tatttgctgg gcattaaagg
     2581 aggaaaaaag gtttagacaa gtttgatatg gaaaagaggg cagtgaatga gaaaagacag
     2641 aaaaatgtga gaagattgtg tatgactgag gaatgaagaa gggagaaaga acctaaacca
     2701 caaggatgtg ggtatatagt aaaagacatg tggacttcat gagtatatat gtgatgtacg
     2761 tattcacagt ttgctcatct gtggaatatg agttgctaga atgctaagtg tattgctatt
     2821 tgtttttccc actgttattt cagcttatga atataaatca aggcaaacat tttaccagag
     2881 cattccaagt tctaatcata tggcttattt tttccctaaa ttgtattttt ttctcaattt
     2941 cttaacaata ttaaactgta agtcatagga taaactggct gaatttattc ttagctgatt
     3001 ctccctcctt tcagatacct ttctagtcca atcaaatttt acataacata cctagctatc
     3061 acaacaaagc aatttagaaa tgagttccca aatactaact ctgtgacagt aggtaaatta
     3121 gatccagagt tttaattgtt gaaaaaacaa gacttaaaaa gaaggttacg atatcatgct
     3181 ataagtagtg atgtttggca ttcagcaata ggttaaacag gtcaagaaca tgggactata
     3241 agaaaaaaac aacaaaaaag aaaaatcaaa gagaaggaaa agggagaata ggatcaaaat
     3301 tcagaaagca tatggtcttt cttcttcctc ttctttgaca ggaaataaaa gtgatgaatg
     3361 agcaggaaaa atatcaatag caaacactca agactatgac cttgaaatgt gcctttatgt
     3421 tgtttctttt aaatgtttaa ctttttttaa ttaaatgaag tgtttatagt cactgctaga
     3481 catttcatca ttatcaaagg cctattatgt cttatacaaa tgtctattta agcattttta
     3541 gcttaaaaat ataaaatcct ttctgtaatt ttctgccttt ttttcatttt caaatatcat
     3601 tctttcaata aaattatctt ccagtaattt tgatgttatt aatcacgatt attttttcat
     3661 cgtgtattat gcctaccagc cacttcatgt atagtatttc acttaaatct tatgaaatca
     3721 acacgattga tattactttt atttcatagc tacagaaatt gaaattcaaa tttcgtccat
     3781 tacaacagac tagctgtggc aaagacaatg tgaatttacc caaatgactt aactcttttt
     3841 ataaattcgt aaaatgtgaa gaatcataga acacacctta aaatgattgt gaaatcaagg
     3901 aggagaaagc tagctaaact ggtagacaaa ctgcaatagg acttgtcaag ccaaaaccta
     3961 caattattct tttggatagt ttctttttct ctaaagtggc aatttaactt aaagatatta
     4021 aagaaaccca atctatgggg ttacttaaat gttctcttcc agcccttcct ctttccagtt
     4081 ttaccaagaa gcacatttta gacaggggaa gagaccaaac caatgacagc agaattacca
     4141 ttatctctag cactattcgt tgtcaaataa ctaaatgctg gtttgaagaa ctaagtaatt
     4201 tgagaaatta aaaatgaaag gaattataaa tgatttgaaa aaaatccttc ttatttttaa
     4261 tgttttttgg tgctgttttc tatgtccttc ctcctatggt gtcaattata tttttttccg
     4321 tgaagtgatt tggttggtga gtctaatctc caacagtgcc ctacctcttt ctcccaaaaa
     4381 ggcctcagtt gaaaaatggc tcaacacttt ccctgcaagc tcacaatgag gatctcctat
     4441 tctccctgca ttcggccaga ctcttctttt tttgcatgct ttcctctccc atgcttgggt
     4501 tgcacagagc taccgcacat ctgtcttcta ggtcaggcta cagagtactt cggatacagc
     4561 aagttacgac ccatctggaa agacagatct acctgccttt tggctttctt ccttggtcaa
     4621 aactaaaatc cgaagtggag tgccccgagc gatagagaaa gtgcctatag ctttgacagc
     4681 cgcttggagc atgctcagtc ggtctccagg caccgggagg cggctccccc cccaccaccc
     4741 cccgggccgg cctccaccgc agcctgtccg gcccccaccc ctggcgcgcg ctcctccgca
     4801 cccacaaggg cgttcagcag ccgcgcctcc ccttcccgcc accgcctcct tctccgctgg
     4861 ctgtggcggg cgaggacacg tcaggggcca taaataaata aataattcca ttcacccgtt
     4921 gacagcgagg cgaatcggct gcggtttgct gggaacggag ggctgcagcg ccaagcttgg
     4981 cattccggta ctgttggtaa aatggaagac gccaaaaaca taaagaaagg cccggcgcca
     5041 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     5101 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
     5161 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     5221 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     5281 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     5341 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
     5401 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
     5461 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     5521 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
     5581 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
     5641 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     5701 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     5761 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     5821 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
     5881 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     5941 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     6001 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     6061 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     6121 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     6181 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     6241 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     6301 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     6361 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc
     6421 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     6481 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     6541 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     6601 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta
     6661 actgtattca gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg
     6721 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa
     6781 taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac
     6841 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     6901 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     6961 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     7021 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     7081 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     7141 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     7201 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     7261 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     7321 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     7381 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     7441 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     7501 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     7561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gtcgaccgat
     7681 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     7741 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     7801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8401 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8641 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9061 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9121 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9181 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9241 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9301 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9361 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9421 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9481 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9541 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9601 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9661 acatatttga atgtatttag aaaaataaac aaataggggt tc
//
