LOCUS       sequence-173898-        8730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, 
            Dave AM, Kiousis S, Sloan AE, Mathupala SP
  TITLE     pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity 
            Monocarboxylate Transporter Expression in Glioblastoma.
  JOURNAL   Neoplasia. 2017 Jan 13;19(2):121-134. doi: 
            10.1016/j.neo.2016.12.011.
  PUBMED    28092823
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2016.12"; journalName: "Neoplasia"; date: 
            "2017-01-13- 13"; volume: "19"; issue: "2"; pages: "121-134"
COMMENT     Sequence Label: pGL2-MCT2Prom3.2
FEATURES             Location/Qualifiers
     source          1..8730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    652..773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4006..5658
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4072..4090)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5899..5964
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6094..6114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6539..6673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6815..6832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6986..7574)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7066..7085)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7745..8536,8537..8605))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8368..8387
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8606..8710)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
      541 taccatgata agtaagtaat attaaggtac gtggaggttt tacttgcttt aaaaaacctc
      601 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      661 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      721 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatggtact
      781 gtaactgagc taacataaat gaaagcagga gtactaggaa agtaaaggaa caaaaaaatg
      841 gctacaccat aggcagagta gcagcatggg ctgctccgct gcttatactt attattacct
      901 cttgattata tgttaacaag agatggatta ttcatgagtt ttctgggaaa ggggtgggaa
      961 attctgggaa ctgagggttt ctccccattt tagaccatat ggggtaactt cctgacctta
     1021 ccatggcatt tgtaaactgt catggcactg gtgggaatgt cttttagcat gctaatgcat
     1081 tgtaattagc acataatgag gagtgaggag gaccagaggt cactttcgtc tccattttgg
     1141 ttttggtggg ttttagctgg cttctttact gccgcctgtt ttatcagcat ggtctttatg
     1201 acctgtatct tgtgccaacc tcctatctta ttctgtgact aagaatgcct taatctcctg
     1261 ggaatgcagc ccaataagtc tcagcctcat tttacccagg ccttactcaa gatggagtca
     1321 ctctggttca aatgcctgtg acaatcccag agatgaataa aatgtcatct ctactttcaa
     1381 atagcctaat atattggggc tacagaaaca aaaatcatat tataatatag tcatatgtga
     1441 agctgaaaca aaatgcccca gatagaggcc taaatcattt tctatgggca ggatcacaga
     1501 gaaagatgaa cttaaccctt atttgttaac aaagaatcct taaaatgcta tagaagtgat
     1561 gatatatttg ctgggcatta aaggaggaaa aaaggtttag acaagtttga tatggaaaag
     1621 agggcagtga atgagaaaag acagaaaaat gtgagaagat tgtgtatgac tgaggaatga
     1681 agaagggaga aagaacctaa accacaagga tgtgggtata tagtaaaaga catgtggact
     1741 tcatgagtat atatgtgatg tacgtattca cagtttgctc atctgtggaa tatgagttgc
     1801 tagaatgcta agtgtattgc tatttgtttt tcccactgtt atttcagctt atgaatataa
     1861 atcaaggcaa acattttacc agagcattcc aagttctaat catatggctt attttttccc
     1921 taaattgtat ttttttctca atttcttaac aatattaaac tgtaagtcat aggataaact
     1981 ggctgaattt attcttagct gattctccct cctttcagat acctttctag tccaatcaaa
     2041 ttttacataa catacctagc tatcacaaca aagcaattta gaaatgagtt cccaaatact
     2101 aactctgtga cagtaggtaa attagatcca gagttttaat tgttgaaaaa acaagactta
     2161 aaaagaaggt tacgatatca tgctataagt agtgatgttt ggcattcagc aataggttaa
     2221 acaggtcaag aacatgggac tataagaaaa aaacaacaaa aaagaaaaat caaagagaag
     2281 gaaaagggag aataggatca aaattcagaa agcatatggt ctttcttctt cctcttcttt
     2341 gacaggaaat aaaagtgatg aatgagcagg aaaaatatca atagcaaaca ctcaagacta
     2401 tgaccttgaa atgtgccttt atgttgtttc ttttaaatgt ttaacttttt ttaattaaat
     2461 gaagtgttta tagtcactgc tagacatttc atcattatca aaggcctatt atgtcttata
     2521 caaatgtcta tttaagcatt tttagcttaa aaatataaaa tcctttctgt aattttctgc
     2581 ctttttttca ttttcaaata tcattctttc aataaaatta tcttccagta attttgatgt
     2641 tattaatcac gattattttt tcatcgtgta ttatgcctac cagccacttc atgtatagta
     2701 tttcacttaa atcttatgaa atcaacacga ttgatattac ttttatttca tagctacaga
     2761 aattgaaatt caaatttcgt ccattacaac agactagctg tggcaaagac aatgtgaatt
     2821 tacccaaatg acttaactct ttttataaat tcgtaaaatg tgaagaatca tagaacacac
     2881 cttaaaatga ttgtgaaatc aaggaggaga aagctagcta aactggtaga caaactgcaa
     2941 taggacttgt caagccaaaa cctacaatta ttcttttgga tagtttcttt ttctctaaag
     3001 tggcaattta acttaaagat attaaagaaa cccaatctat ggggttactt aaatgttctc
     3061 ttccagccct tcctctttcc agttttacca agaagcacat tttagacagg ggaagagacc
     3121 aaaccaatga cagcagaatt accattatct ctagcactat tcgttgtcaa ataactaaat
     3181 gctggtttga agaactaagt aatttgagaa attaaaaatg aaaggaatta taaatgattt
     3241 gaaaaaaatc cttcttattt ttaatgtttt ttggtgctgt tttctatgtc cttcctccta
     3301 tggtgtcaat tatatttttt tccgtgaagt gatttggttg gtgagtctaa tctccaacag
     3361 tgccctacct ctttctccca aaaaggcctc agttgaaaaa tggctcaaca ctttccctgc
     3421 aagctcacaa tgaggatctc ctattctccc tgcattcggc cagactcttc tttttttgca
     3481 tgctttcctc tcccatgctt gggttgcaca gagctaccgc acatctgtct tctaggtcag
     3541 gctacagagt acttcggata cagcaagtta cgacccatct ggaaagacag atctacctgc
     3601 cttttggctt tcttccttgg tcaaaactaa aatccgaagt ggagtgcccc gagcgataga
     3661 gaaagtgcct atagctttga cagccgcttg gagcatgctc agtcggtctc caggcaccgg
     3721 gaggcggctc cccccccacc accccccggg ccggcctcca ccgcagcctg tccggccccc
     3781 acccctggcg cgcgctcctc cgcacccaca agggcgttca gcagccgcgc ctccccttcc
     3841 cgccaccgcc tccttctccg ctggctgtgg cgggcgagga cacgtcaggg gccataaata
     3901 aataaataat tccattcacc cgttgacagc gaggcgaatc ggctgcggtt tgctgggaac
     3961 ggagggctgc agcgccaagc ttggcattcc ggtactgttg gtaaaatgga agacgccaaa
     4021 aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc tggagagcaa
     4081 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
     4141 catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa
     4201 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
     4261 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
     4321 aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca gcctaccgta
     4381 gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata
     4441 atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
     4501 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt accagagtcc
     4561 tttgatcgtg acaaaacaat tgcactgata atgaattcct ctggatctac tgggttacct
     4621 aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc cagagatcct
     4681 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
     4741 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     4801 tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat tcaaagtgcg
     4861 ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga caaatacgat
     4921 ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa
     4981 gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct cactgagact
     5041 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     5101 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     5161 cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt aaacaatccg
     5221 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     5281 tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat taaatacaaa
     5341 ggatatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc caacatcttc
     5401 gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     5461 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     5521 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     5581 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     5641 ggaaagtcca aattgtaaaa tgtaactgta ttcagcgatg acgaaattct tagctattgt
     5701 aatactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
     5761 aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcgc
     5821 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     5881 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     5941 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     6001 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     6061 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     6121 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     6181 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     6241 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     6301 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     6361 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     6421 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6481 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     6541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6661 tcatgtctgg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     6721 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     6781 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6901 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7021 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7081 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7141 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7201 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7261 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7321 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7381 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7441 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7501 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7561 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7621 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7681 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7741 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7801 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7861 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7921 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7981 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8041 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8101 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8161 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8221 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8281 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8341 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8401 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8461 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8521 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8581 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8641 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8701 gggttccgcg cacatttccc cgaaaagtgc
//
