LOCUS       sequence-17390-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, 
            Simonitch TA, Hack FM, Lawrence HJ
  TITLE     Lineage-restricted expression of homeobox-containing genes in human 
            hematopoietic cell lines.
  JOURNAL   Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40.
  PUBMED    2573064
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1989-11"; volume: "86(21)"; pages: "8536-40"
COMMENT     Sequence Label: HOXB6 (human) FLAG pCMV
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..51
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             134..145
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtcagatccg ctagcgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag
       61 ctggagctcc accgcggtgg cggccgccac catggattac aaggatgacg acgataagag
      121 cccgggcgga tccatcgagg gtaggctgag ctcctatttc gttaactcca ccttccccgt
      181 cactctggcc agcgggcagg agtccttcct gggccagcta ccgctctatt cgtcgggcta
      241 tgcggacccg ctgagacatt accccgcgcc ctacgggcca gggccgggcc aggacaaggg
      301 ctttgccact tcctcctatt acccgccggc gggcggtggc tacggccgag cggcgccctg
      361 cgactacggg ccggcgccgg ccttctaccg cgagaaagag tcggcctgcg cactctccgg
      421 cgccgacgag cagcccccgt tccaccccga gccgcggaag tcggactgcg cgcaggacaa
      481 gagcgtgttc ggcgagacag aagagcagaa gtgctccact ccggtctacc cgtggatgca
      541 gcggatgaat tcgtgcaaca gttcctcctt tgggcccagc ggccggcgag gccgccagac
      601 atacacacgt taccagacgc tggagctgga gaaggagttt cactacaatc gctacctgac
      661 gcggcggcgg cgcatcgaga tcgcgcacgc cctgtgcctg acggagaggc agatcaagat
      721 atggttccag aaccgacgca tgaagtggaa aaaggagagc aaactgctca gcgcgtctca
      781 gctcagtgcc gaggaggagg aagaaaaaca ggccgagtga aggtgctgga aagggaggga
      841 ggacgcgagg ggaaaggcct gtggggagcc acggggcgtc agagagaccc gggaaggaaa
      901 gctctcgggt gggggagcca ggagacctgc tctcgggcgc agacaggcgg ggccagcgct
      961 ctccttggaa gcccccgccc
//
