LOCUS       sequence-173901-        6633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, 
            Dave AM, Kiousis S, Sloan AE, Mathupala SP
  TITLE     pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity 
            Monocarboxylate Transporter Expression in Glioblastoma.
  JOURNAL   Neoplasia. 2017 Jan 13;19(2):121-134. doi: 
            10.1016/j.neo.2016.12.011.
  PUBMED    28092823
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2016.12"; journalName: "Neoplasia"; date: 
            "2017-01-13- 13"; volume: "19"; issue: "2"; pages: "121-134"
COMMENT     Sequence Label: pGL2-MCT2Prom1.1
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(106..125)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     316..337
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    664..785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1921..3573
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1987..2005)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3814..3879
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4009..4029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4454..4588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4730..4747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4901..5489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4981..5000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5660..6451,6452..6520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6283..6302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6521..6625)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
       61 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      241 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      301 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      421 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgccatt
      481 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      541 gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt tttacttgct
      601 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      661 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      721 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      781 tcttatggta ctgtaactga gctaacataa cccgggagga ggagaaagct agctaaactg
      841 gtagacaaac tgcaatagga cttgtcaagc caaaacctac aattattctt ttggatagtt
      901 tctttttctc taaagtggca atttaactta aagatattaa agaaacccaa tctatggggt
      961 tacttaaatg ttctcttcca gcccttcctc tttccagttt taccaagaag cacattttag
     1021 acaggggaag agaccaaacc aatgacagca gaattaccat tatctctagc actattcgtt
     1081 gtcaaataac taaatgctgg tttgaagaac taagtaattt gagaaattaa aaatgaaagg
     1141 aattataaat gatttgaaaa aaatccttct tatttttaat gttttttggt gctgttttct
     1201 atgtccttcc tcctatggtg tcaattatat ttttttccgt gaagtgattt ggttggtgag
     1261 tctaatctcc aacagtgccc tacctctttc tcccaaaaag gcctcagttg aaaaatggct
     1321 caacactttc cctgcaagct cacaatgagg atctcctatt ctccctgcat tcggccagac
     1381 tcttcttttt ttgcatgctt tcctctccca tgcttgggtt gcacagagct accgcacatc
     1441 tgtcttctag gtcaggctac agagtacttc ggatacagca agttacgacc catctggaaa
     1501 gacagatcta cctgcctttt ggctttcttc cttggtcaaa actaaaatcc gaagtggagt
     1561 gccccgagcg atagagaaag tgcctatagc tttgacagcc gcttggagca tgctcagtcg
     1621 gtctccaggc accgggaggc ggctcccccc ccaccacccc ccgggccggc ctccaccgca
     1681 gcctgtccgg cccccacccc tggcgcgcgc tcctccgcac ccacaagggc gttcagcagc
     1741 cgcgcctccc cttcccgcca ccgcctcctt ctccgctggc tgtggcgggc gaggacacgt
     1801 caggggccat aaataaataa ataattccat tcacccgttg acagcgaggc gaatcggctg
     1861 cggtttgctg ggaacggagg gctgcagcgc caagcttggc attccggtac tgttggtaaa
     1921 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga
     1981 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
     2041 gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
     2101 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     2161 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     2221 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt
     2281 tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     2341 aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga
     2401 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     2461 tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga
     2521 tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg
     2581 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     2641 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     2701 cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac
     2761 aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg
     2821 attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg
     2881 aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat
     2941 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     3001 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     3061 acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt
     3121 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     3181 ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct
     3241 ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa
     3301 caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt
     3361 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     3421 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     3481 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     3541 aaggccaaga agggcggaaa gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa
     3601 attcttagct attgtaatac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     3661 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
     3721 atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     3781 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     3841 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     3901 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     3961 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     4021 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     4081 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     4141 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     4201 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     4261 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     4321 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     4381 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4441 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4501 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4561 catcaatgta tcttatcatg tctggatccg tcgaccgatg cccttgagag ccttcaaccc
     4621 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     4681 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     4741 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4801 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4861 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4921 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4981 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5041 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5101 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5161 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5221 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5281 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5341 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5401 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5461 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5521 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5581 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5641 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5701 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5761 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5821 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5881 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5941 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6001 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6061 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6121 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6181 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6241 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6301 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6361 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6421 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6481 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6541 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6601 aaaataaaca aataggggtt ccgcgcacat ttc
//
