LOCUS       sequence-173903-        6131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, 
            Dave AM, Kiousis S, Sloan AE, Mathupala SP
  TITLE     pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity 
            Monocarboxylate Transporter Expression in Glioblastoma.
  JOURNAL   Neoplasia. 2017 Jan 13;19(2):121-134. doi: 
            10.1016/j.neo.2016.12.011.
  PUBMED    28092823
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2016.12"; journalName: "Neoplasia"; date: 
            "2017-01-13- 13"; volume: "19"; issue: "2"; pages: "121-134"
COMMENT     Sequence Label: pGL2-MCT2Prom0.6
FEATURES             Location/Qualifiers
     source          1..6131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(125..144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     335..356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    683..804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1438..3090
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1504..1522)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3331..3396
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3526..3546
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3971..4105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4247..4264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4418..5006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4498..4517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5177..5968,5969..6037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5800..5819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6038..6131,1..11))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
       61 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      241 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      361 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
      481 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
      541 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
      601 cgtggaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatggtac tgtaactgag ctaacataac ccgggctcaa
      841 cactttccct gcaagctcac aatgaggatc tcctattctc cctgcattcg gccagactct
      901 tctttttttg catgctttcc tctcccatgc ttgggttgca cagagctacc gcacatctgt
      961 cttctaggtc aggctacaga gtacttcgga tacagcaagt tacgacccat ctggaaagac
     1021 agatctacct gccttttggc tttcttcctt ggtcaaaact aaaatccgaa gtggagtgcc
     1081 ccgagcgata gagaaagtgc ctatagcttt gacagccgct tggagcatgc tcagtcggtc
     1141 tccaggcacc gggaggcggc tcccccccca ccaccccccg ggccggcctc caccgcagcc
     1201 tgtccggccc ccacccctgg cgcgcgctcc tccgcaccca caagggcgtt cagcagccgc
     1261 gcctcccctt cccgccaccg cctccttctc cgctggctgt ggcgggcgag gacacgtcag
     1321 gggccataaa taaataaata attccattca cccgttgaca gcgaggcgaa tcggctgcgg
     1381 tttgctggga acggagggct gcagcgccaa gcttggcatt ccggtactgt tggtaaaatg
     1441 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc
     1501 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
     1561 tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt
     1621 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
     1681 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
     1741 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg
     1801 cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
     1861 aaattaccaa taatccagaa aattattatc atggattcta aaacggatta ccagggattt
     1921 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
     1981 gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct
     2041 actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat
     2101 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
     2161 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
     2221 gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa
     2281 attcaaagtg cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt
     2341 gacaaatacg atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa
     2401 gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg
     2461 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
     2521 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
     2581 ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat
     2641 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
     2701 gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta
     2761 attaaataca aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac
     2821 cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc
     2881 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
     2941 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
     3001 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
     3061 gccaagaagg gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt
     3121 cttagctatt gtaatactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     3181 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     3241 gcagaaattc gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     3301 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     3361 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     3421 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     3481 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     3541 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     3601 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     3661 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     3721 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     3781 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     3841 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     3901 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3961 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4021 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4081 caatgtatct tatcatgtct ggatccgtcg accgatgccc ttgagagcct tcaacccagt
     4141 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     4201 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
     4261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6121 ataaacaaat a
//
