LOCUS       sequence-173908-        8312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  disruption of human RMRP gene via CRISPR-Cas9, guide 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Goldfarb KC, Cech TR
  TITLE     Targeted CRISPR disruption reveals a role for RNase MRP RNA in human
            preribosomal RNA processing.
  JOURNAL   Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub
            2017 Jan 23.
  PUBMED    28115465
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.286963.116"; journalName: "Genes Dev"; date: 
            "2017-01-1- 1"; volume: "31"; issue: "1"; pages: "59-71"
COMMENT     Sequence Label: px330-MRPsg3
FEATURES             Location/Qualifiers
     source          1..8312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      213..222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             222..287
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             294..314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             339..4439
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             339..4439
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4440..4487
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4515..4532)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4521..4728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4737..4877
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4737..4866
                     /label=AAV2 ITR
     rep_origin      4952..5407
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5039..5058)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5249..5270
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5424..5443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5543..5565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5603..5621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5689..5793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5794..5862,5863..6654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6012..6031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6825..7413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7314..7333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7475..7723
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7475..7495
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7646..7665
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7744..7819
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        7916..8201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
       61 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg
      121 taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg
      181 cctgaaatca ctttttttca ggttggaccg gtgccaccat ggactataag gaccacgacg
      241 gagactacaa ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga
      301 agaagcggaa ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcggcc
      361 tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
      421 gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg
      481 gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca
      541 gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg
      601 agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag
      661 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc
      721 acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg
      781 ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc
      841 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg
      901 tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca
      961 aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc
     1021 tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga
     1081 cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg
     1141 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc
     1201 tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga
     1261 acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc
     1321 accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag
     1381 agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc
     1441 aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac
     1501 tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca
     1561 gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt
     1621 tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc
     1681 cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga
     1741 gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc
     1801 agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc
     1861 ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat
     1921 acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     1981 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     2041 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     2101 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     2161 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     2221 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     2281 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     2341 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     2401 ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     2461 agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     2521 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     2581 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     2641 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     2701 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     2761 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     2821 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     2881 acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca
     2941 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     3001 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     3061 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     3121 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     3181 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     3241 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     3301 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     3361 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     3421 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     3481 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     3541 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     3601 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     3661 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     3721 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     3781 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     3841 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     3901 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     3961 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     4021 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     4081 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     4141 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     4201 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     4261 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     4321 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     4381 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca
     4441 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagtaa gaattcctag
     4501 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4561 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4621 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4681 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgcagga
     4741 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4801 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4861 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4921 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4981 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     5041 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5101 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5161 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5221 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5281 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     5341 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     5401 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5461 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5521 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5581 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5641 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5701 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5761 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5821 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5881 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5941 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6001 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6061 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6121 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6181 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6241 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6301 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6361 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6421 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6481 gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc actggggcca
     6541 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6601 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6661 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7441 ttcctggcct tttgctggcc ttttgctcac atgtgagggc ctatttccca tgattccttc
     7501 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     7561 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     7621 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     7681 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgctcagc gggatacgct
     7741 tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     7801 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc
     7861 tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag gtacccgtta
     7921 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7981 caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     8041 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     8101 atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta
     8161 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     8221 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     8281 tttaattatt ttgtgcagcg atgggggcgg gg
//
