LOCUS       sequence-173924-        8792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human 
            IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo 
            (Universal)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, 
            Nakayama K
  TITLE     Practical method for targeted disruption of cilia-related genes by 
            using CRISPR/Cas9-mediated homology-independent knock-in system.
  JOURNAL   Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 
            10.1091/mbc.E17-01-0051.
  PUBMED    28179459
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 
            10.1091/mbc.E17-01-0051."
COMMENT     Sequence Label: peSpCas9(1.1)-2×sgRNA (IFT88, donor)
FEATURES             Location/Qualifiers
     source          1..8792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          30..257
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             278..343
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             350..370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(395..2932,2933..2935,2936..3397,3398..3400,3401..3568,
                     3569..3571,3572..4495)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4496..4543
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4571..4588)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4577..4784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4793..4933
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4793..4922
                     /label=AAV2 ITR
     rep_origin      5008..5463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5095..5114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5305..5326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5480..5499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5599..5621
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5659..5677)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5745..5849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5850..5918,5919..6710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6068..6087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6881..7469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7370..7389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7531..7779
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        7800..7875
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7966..8214
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        8235..8310
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8401..8686
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
       61 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
      121 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagctgagc aagaggtaag
      181 ggtttaaggg atggttggtt ggtggggtat taatgtttaa ttacctggag cacctgcctg
      241 aaatcacttt ttttcaggtt ggaccggtgc caccatggac tataaggacc acgacggaga
      301 ctacaaggat catgatattg attacaaaga cgatgacgat aagatggccc caaagaagaa
      361 gcggaaggtc ggtatccacg gagtcccagc agccgacaag aagtacagca tcggcctgga
      421 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
      481 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
      541 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
      601 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
      661 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
      721 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
      781 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
      841 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
      901 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
      961 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     1021 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     1081 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     1141 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     1201 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     1261 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     1321 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     1381 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     1441 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     1501 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     1561 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     1621 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     1681 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     1741 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     1801 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     1861 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     1921 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     1981 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     2041 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     2101 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     2161 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     2221 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     2281 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     2341 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     2401 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     2461 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     2521 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     2581 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     2641 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     2701 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     2761 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     2821 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     2881 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tggccgacga
     2941 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     3001 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     3061 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     3121 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     3181 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     3241 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     3301 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     3361 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctgcg ctggaaagcg agttcgtgta
     3421 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     3481 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     3541 taccctggcc aacggcgaga tccggaaggc gcctctgatc gagacaaacg gcgaaaccgg
     3601 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     3661 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     3721 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     3781 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     3841 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     3901 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     3961 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     4021 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     4081 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     4141 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     4201 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     4261 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     4321 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     4381 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     4441 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacaaaag
     4501 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagtaagaat tcctagagct
     4561 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4621 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4681 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4741 agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc gcaggaaccc
     4801 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4861 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4921 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4981 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     5041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     5101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5221 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     5341 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     5401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5461 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5521 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5581 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5641 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5701 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5761 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5821 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5881 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5941 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6001 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6061 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6121 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6181 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6241 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6301 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6361 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6421 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6481 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6541 ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg
     6601 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6661 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6721 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6781 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6841 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6901 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6961 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7021 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7081 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7141 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7201 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7261 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7321 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7381 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7441 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7501 tggccttttg ctggcctttt gctcacatgt gagggcctat ttcccatgat tccttcatat
     7561 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
     7621 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     7681 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     7741 ttcttggctt tatatatctt gtggaaagga cgaaacaccg ggccccttga ctgactaagg
     7801 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     7861 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
     7921 ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagagaggg cctatttccc
     7981 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     8041 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     8101 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     8161 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcatcg
     8221 tacgcgtacg tgttgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     8281 caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga aatagcaagt
     8341 taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa tggcggtacc
     8401 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     8461 gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8521 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     8581 cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt
     8641 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     8701 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     8761 tattttttaa ttattttgtg cagcgatggg gg
//
