LOCUS       sequence-173930-        7206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human VEGFR1 with deletion of the entire intracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Raikwar NS, Liu KZ, Thomas CP
  TITLE     N-terminal cleavage and release of the ectodomain of Flt1 is 
            mediated via ADAM10 and ADAM 17 and regulated by VEGFR2 and the Flt1
            intracellular domain.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112794. doi: 
            10.1371/journal.pone.0112794. eCollection 2014.
  PUBMED    25387128
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112794. doi"; pages: " 
            10.1371/journal.pone.0112794. eCollection 2014"
COMMENT     Sequence Label: hFLT1 delta CTD
FEATURES             Location/Qualifiers
     source          1..7206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1119..1145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3420..3443
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3463..3479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3463..3479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3490..4112
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3689..3708)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(4261..4849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4341..4360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5178..5225
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5250..5269
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5457..6251)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5457..6251)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5568..5587)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6178..6197
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6286..6643)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6300..6434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6452..6643
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6600..6620
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(6645..6749)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6776..7204
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6863..6882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7073..7094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggtcagctac tgggacaccg gggtcctgct gtgcgcgctg ctcagctgtc
     1081 tgcttctcac aggatctagt tcaggttcaa aattaaaata cccatatgac gtcccagact
     1141 acgctgatcc tgaactgagt ttaaaaggca cccagcacat catgcaagca ggccagacac
     1201 tgcatctcca atgcaggggg gaagcagccc ataaatggtc tttgcctgaa atggtgagta
     1261 aggaaagcga aaggctgagc ataactaaat ctgcctgtgg aagaaatggc aaacaattct
     1321 gcagtacttt aaccttgaac acagctcaag caaaccacac tggcttctac agctgcaaat
     1381 atctagctgt acctacttca aagaagaagg aaacagaatc tgcaatctat atatttatta
     1441 gtgatacagg tagacctttc gtagagatgt acagtgaaat ccccgaaatt atacacatga
     1501 ctgaaggaag ggagctcgtc attccctgcc gggttacgtc acctaacatc actgttactt
     1561 taaaaaagtt tccacttgac actttgatcc ctgatggaaa acgcataatc tgggacagta
     1621 gaaagggctt catcatatca aatgcaacgt acaaagaaat agggcttctg acctgtgaag
     1681 caacagtcaa tgggcatttg tataagacaa actatctcac acatcgacaa accaatacaa
     1741 tcatagatgt ccaaataagc acaccacgcc cagtcaaatt acttagaggc catactcttg
     1801 tcctcaattg tactgctacc actcccttga acacgagagt tcaaatgacc tggagttacc
     1861 ctgatgaaaa aaataagaga gcttccgtaa ggcgacgaat tgaccaaagc aattcccatg
     1921 ccaacatatt ctacagtgtt cttactattg acaaaatgca gaacaaagac aaaggacttt
     1981 atacttgtcg tgtaaggagt ggaccatcat tcaaatctgt taacacctca gtgcatatat
     2041 atgataaagc attcatcact gtgaaacatc gaaaacagca ggtgcttgaa accgtagctg
     2101 gcaagcggtc ttaccggctc tctatgaaag tgaaggcatt tccctcgccg gaagttgtat
     2161 ggttaaaaga tgggttacct gcgactgaga aatctgctcg ctatttgact cgtggctact
     2221 cgttaattat caaggacgta actgaagagg atgcagggaa ttatacaatc ttgctgagca
     2281 taaaacagtc aaatgtgttt aaaaacctca ctgccactct aattgtcaat gtgaaacccc
     2341 agatttacga aaaggccgtg tcatcgtttc cagacccggc tctctaccca ctgggcagca
     2401 gacaaatcct gacttgtacc gcatatggta tccctcaacc tacaatcaag tggttctggc
     2461 acccctgtaa ccataatcat tccgaagcaa ggtgtgactt ttgttccaat aatgaagagt
     2521 cctttatcct ggatgctgac agcaacatgg gaaacagaat tgagagcatc actcagcgca
     2581 tggcaataat agaaggaaag aataagatgg ctagcacctt ggttgtggct gactctagaa
     2641 tttctggaat ctacatttgc atagcttcca ataaagttgg gactgtggga agaaacataa
     2701 gcttttatat cacagatgtg ccaaatgggt ttcatgttaa cttggaaaaa atgccgacgg
     2761 aaggagagga cctgaaactg tcttgcacag ttaacaagtt cttatacaga gacgttactt
     2821 ggattttact gcggacagtt aataacagaa caatgcacta cagtattagc aagcaaaaaa
     2881 tggccatcac taaggagcac tccatcactc ttaatcttac catcatgaat gtttccctgc
     2941 aagattcagg cacctatgcc tgcagagcca ggaatgtata cacaggggaa gaaatcctcc
     3001 agaagaaaga aattacaatc agagatcagg aagcaccata cctcctgcga aacctcagtg
     3061 atcacacagt ggccatcagc agttccacca ctttagactg tcatgctaat ggtgtccccg
     3121 agcctcagat cacttggttt aaaaacaacc acaaaataca acaagagcct ggaattattt
     3181 taggaccagg aagcagcacg ctgtttattg aaagagtcac agaagaggat gaaggtgtct
     3241 atcactgcaa agccaccaac cagaagggct ctgtggaaag ttcagcatac ctcactgttc
     3301 aaggaacctc ggacaagtct aatctggagc tgatcactct aacatgcacc tgtgtggctg
     3361 cgactctctt ctggctccta ttaaccctct ttatccgaaa aatgaaaagg gatatcctgg
     3421 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     3481 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     3541 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     3601 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     3661 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     3721 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     3781 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     3841 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     3901 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     3961 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     4021 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     4081 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     4141 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4441 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4681 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4921 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4981 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     5041 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     5101 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     5161 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     5221 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     5281 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5341 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5401 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     5461 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5521 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5581 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     5641 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     5701 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     5761 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     5821 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     5881 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     5941 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6001 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6061 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6121 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6181 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6241 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     6301 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     6361 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6421 agttaggggc gggactrtgg gagttagggg cgggactatg gttgctgact aattgagatg
     6481 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6541 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6601 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     6661 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6721 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     6781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6841 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6901 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6961 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7021 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7081 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7141 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7201 aatttt
//
