LOCUS       sequence-173931-        3617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibile Rpa Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Prpa
FEATURES             Location/Qualifiers
     source          1..3617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             48..761
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(791..808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(870..969)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(986..1005)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(987..1005)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1010..1026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1010..1026)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1139..1243
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1244..1312,1313..2104)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1462..1481)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2682..2698
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2714..2813
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     3198..3214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3230..3329
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttcccgttcg cacctgcact gttcccgcct gcaaaggagg tgacaatatg cgtaaaggcg
       61 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      121 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      181 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
      241 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
      301 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
      361 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
      421 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
      481 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
      541 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
      601 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
      661 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
      721 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaggatcc taactcgctc
      781 ctcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      841 cagctcactc aaaggcggta atcaattcga cccagctttc ttgtacaaag ttggcattat
      901 aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      961 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     1021 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1081 tatatcatca tgcctcctct agacttagac gtcaggtggc acttttcggg gaaatgtgcg
     1141 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1201 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1261 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1321 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1381 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1441 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1501 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1561 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1621 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1681 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1741 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1801 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1861 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1921 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1981 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2041 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2101 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2161 atttaaaagg atctaggtga agatcctttt tgataatcct cgaggagcgc ctcagcgcgc
     2221 cgtagcgtcg ataaaaatta cgggctgggg cgaaactacc atctgttcga aaaggtccgt
     2281 aaatgggcct acagagcgat tcgtcagggc tggcctgtat tctcacaatg gcttgatgcc
     2341 gttatccagc gtgtcgaaat gtacaacgct tcgcttcccg ttccgctttc tccggctgaa
     2401 tgtcgggcta ttggcaagag cattgcgaaa tatacacaca ggaaattctc accagaggga
     2461 ttttccgctg tacaggccgc tcgcggtcgc aagggcggaa ctaaatctaa gcgcgcagca
     2521 gttcctacat cagcacgttc gctgaaaccg tgggaggcat taggcatcag tcgagcgacg
     2581 tactaccgaa aattaaaatg tgacccagac ctcgcaaaat gagaccagat aagccttatc
     2641 agataacagc gcccttttgg cgtctttttg agcacctagg tgtaaaacga cggccagtct
     2701 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
     2761 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag gctccgaatt
     2821 ggtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatctgaag
     2881 tggaattcat gagatctacc tgtccgatcg gacagtagtt aggttcccgt tcgcacctgc
     2941 actgttcccg cctgcaaagg aggtgacaat atgcgtaaag gcgaagagct gttcactggt
     3001 gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt
     3061 ggcgagggtg aagcattagg catcagtcga gcgacgtact accgaaaatt aaaatgtgac
     3121 ccagacctcg caaaatgaga ccagataagc cttatcagat aacagcgccc ttttggcgtc
     3181 tttttgagca cctaggtgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
     3241 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
     3301 aatgccaact ttgtacaaaa aagcaggctc cgaattggta tcacgaggca gaatttcaga
     3361 taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga attcatgaga tctacctgtc
     3421 cgatcggaca gtagttaggt tcccgttcgc acctgcactg ttcccgcctg caaaggaggt
     3481 gacaatatgc gtaaaggcga agaggctccg aattggtatc acgaggcaga atttcagata
     3541 aaaaaaatcc ttagctttcg ctaaggatct gaagtggaat tcatgagatc tacctgtccg
     3601 atcggacagt agttagg
//
