LOCUS       sequence-173960-        4580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for a transcript origination from a D. pse core 
            promoter, which is used as trasfection control (normalization), i.e.
            STAP-seq screening vector containing one specific core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, 
            Stark A
  TITLE     Genome-wide assessment of sequence-intrinsic enhancer responsiveness
            at single-base-pair resolution.
  JOURNAL   Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739.
  PUBMED    28024147
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739."
COMMENT     Sequence Label: pSTAP-seq_fly-zfh1_spikeIn-4
FEATURES             Location/Qualifiers
     source          1..4580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    587..635
                     /note="synthetic polyadenylation signal"
     misc_feature    649..740
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     689..708
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1953..1962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1968..2034
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(2079..2107)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     polyA_signal    2274..2395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2285..2304)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2339..2358
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2659..2676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2830..3418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2910..2929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3589..4380,4381..4449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4212..4231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4450..4554)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accttaaaag tgccagtgct tgtggcgtgc
      781 acggagaaaa aaaccttgag gtcatatttg aaaaaaaaaa aaaaaaaact aaattccaag
      841 tgagtagttt actcttagtc actatcgagt gtgtgctgtt tgtttttcga aatatgcata
      901 tcttagctac ttgcgttgct gttttttttt tttttttttt tttgacgatt tgtcaacagg
      961 aaatatgcat aaggttggct cgggatccgg atcggatggc atggggtttg attggatgcc
     1021 gtacttggca ccgcactgcc gttatcggtt ggctcatttc ccgttagccg cgaacaaagc
     1081 ccacgcattg tgctacgacc gccgtgatgt gttcaaagat caagtgcccc cagaaccatc
     1141 tcctgccatc cctgccatcc ctgcccgcct gcatccatcg cgggcattca gattccgact
     1201 gcgcatgagc agcggtctcc aacacgtaag caagtggaag gcgagggata agaacaagta
     1261 acttctctct tatcggacgc ttccaggaat ttcgatcctc gcaccgcatt ccgcaggttt
     1321 agtcactgga ttccacggct tttcgagtcg attgtcccaa gaaacattat tttggacatg
     1381 tgactcactg actcaaagct attatcactt ctcggacata tgcgtgtata taaaatattt
     1441 ttaaacatta ttaggtatac tataaactct gcctcattca tgcctacaga acttcagcct
     1501 tgacattcta tatatagaaa cccttactac gtctttactc tttaaatagt tctttggata
     1561 ttatattagc acattgatgt gctttttttt tggatggttg tgtccaactc acggtaccga
     1621 gctcttacgc gtgctagccc gggctcgaga tcttagagca tgcaccggac actctttccc
     1681 tacacgacgc tcttccgatc tccggccgcc ccagcagcgc ctgccactgc cacactgcca
     1741 ctgcctggtg ccgtgccgtg ccccgagttt cagtttgcca tagaacgcgg accgagcagc
     1801 aagcggacac tcgacactca acactcgaca ctcgacaagc gagagataga gatataatca
     1861 cgttcgtcat acagatcgga agagcacacg tctgaactcc agtcactcga cgaattcggc
     1921 ccctgcaggg gcattccggt actgttggta aagccaccat ggaaaaggta ggttcaacca
     1981 ctgatgccta ggcacaccga aacgactaac cctaattctt atcctttact tcaggccact
     2041 taagcatggc tagcaaagga gaagaactct tcactggagt tgtcccaatt cttgttgaat
     2101 tagatggtga tgttaacggc cacaagttct ctgtcagtgg agagggtgaa ggtgatgcaa
     2161 catacggaaa acttaccctg aagttcatct gcactactgg caaactgcct gttccctggc
     2221 caacactagt ctgatctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     2281 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2341 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2401 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2461 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgaccgatg ccctcaagct
     2521 ttgagagggg cccccttcaa cccagtcagc tccgacgtct tggcgccccc gcggggcatg
     2581 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     2641 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4561 tccccgaaaa gtgccacctg
//
