LOCUS       sequence-174000-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral fluorescent reporter using the neuronal cell 
            type-specific promoter of the CaM Kinase II alpha gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  TITLE     pLenti-CaMKIIalpha-mTagBFP2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-CaMKIIa-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        90..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     332..351
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        467..514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             533..907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1037..1158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1074..1093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1128..1147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1207..1223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1207..1223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1220..1242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1231..1247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1255..1261,1262..1279,1280..1285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1300..1321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1438..1455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1609..2197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1689..1708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2368..3159,3160..3228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2991..3010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3229..3333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3408..3427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3599..3978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3980..4178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4133..4153
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4196..4376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4423..4548
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5041..5274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5459..5503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5801..5918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5963..7251
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             7270..7287
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7297..7998
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8023..8611
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8076..8096)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8494..8505)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8614..8630)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8615..8631)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9136..9316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9342..9359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9348..9572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9618..9970,1..76)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9705..9724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9915..9936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
       61 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
      481 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
      541 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
      601 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
      661 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
      721 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
      781 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
      841 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
      901 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
      961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3361 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3421 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3481 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     3541 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     3601 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     3661 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3721 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3781 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3841 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3901 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3961 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4021 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4081 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4141 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     4201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4381 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     4441 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4501 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     4561 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     4621 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     4681 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     4741 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     4801 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4861 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     4921 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     4981 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5041 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5101 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5161 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     5221 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     5281 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     5341 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     5401 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     5461 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     5521 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     5581 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     5641 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     5701 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     5761 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5821 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5881 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5941 gagatccagt ttggttaatt aacattatgg ccttaggtca cttcatctcc atggggttct
     6001 tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca
     6061 gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca
     6121 tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc
     6181 aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg
     6241 ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg
     6301 aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg
     6361 gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg
     6421 acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta
     6481 cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct
     6541 ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca
     6601 aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg
     6661 taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag
     6721 atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac
     6781 ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta
     6841 aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa
     6901 gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca
     6961 cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga
     7021 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
     7081 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
     7141 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg
     7201 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc
     7261 atggggagcc accatcacca tcaccatggc agatctatga gcgagctgat taaggagaac
     7321 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     7381 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     7441 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     7501 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     7561 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     7621 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     7681 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     7741 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     7801 catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     7861 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     7921 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     7981 gggcacaagc ttaattaaga attcgatatc aagcttatcg ataatcaacc tctggattac
     8041 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8101 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8161 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8221 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8281 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8341 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8401 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8461 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8521 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8581 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     8641 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     8701 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     8761 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8821 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     8881 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     8941 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     9001 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     9061 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     9121 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9301 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     9361 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     9421 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     9481 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     9541 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     9601 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9961 ctattctttt
//
