LOCUS       sequence-174002-        9917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral fluorescent reporter using the neuronal cell 
            type-specific promoter of the CaM Kinase II alpha gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Rodrigues DC, Kim DS, Yang G, Zaslavsky K, Ha KC, Mok RS, Ross PJ, 
            Zhao M, Piekna A, Wei W, Blencowe BJ, Morris Q, Ellis J
  TITLE     MECP2 Is Post-transcriptionally Regulated during Human 
            Neurodevelopment by Combinatorial Action of RNA-Binding Proteins and
            miRNAs.
  JOURNAL   Cell Rep. 2016 Oct 11;17(3):720-734. doi: 
            10.1016/j.celrep.2016.09.049.
  PUBMED    27732849
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-11- 11"; volume: "17"; issue: "3"; pages: "720-734"
COMMENT     Sequence Label: pLenti-CaMaKIIalpha-mKO2
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     265..285
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(294..722)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(404..425)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     616..635
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    768..992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     981..998
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1024..1204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1709..1725
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1710..1726
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1729..2317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1835..1846
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2244..2264
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2357..3013)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     regulatory      3010..3019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3036..4324)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    4369..4486
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4784..4828)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5013..5246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5739..5864
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5911..6091
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6109..6307)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6134..6154)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6309..6688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6860..6879
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6954..7058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7059..7127,7128..7919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7277..7296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8090..8678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8579..8598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8832..8849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8966..8987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9002..9007,9008..9025,9026..9032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9040..9056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9045..9067
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9064..9080
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9064..9080
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9129..9250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9140..9159)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9194..9213
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9380..9754)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(9773..9820)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(9868..9917,1..280))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9882..9917,1..100)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
       61 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      121 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      181 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      241 ctggggagcc tggggacttt ccacacccta actgacacac attccacaga attaattcgc
      301 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      361 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      421 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      481 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
      541 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
      601 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
      661 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
      721 gtggggatac cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccac
      781 cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
      841 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
      901 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
      961 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaacggg
     1021 ccctgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     1081 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     1141 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     1201 acccagtaca gtccggatgc agctctcggg ccatgtgatg aaatgctagg cggctgtcaa
     1261 acctccactc taatacttct ctctccgggt catccatccc atgcaggctc acagggtgta
     1321 acaagcgggt gttctctcct tcattggctt cttctacctt ctcttgctca actggtacta
     1381 gcttgtagca ccatccaaag gtcagtggat atctgatccc tggccctggt gtgtagttct
     1441 gccaatcagg gaagtagcct tgtgtgtggt agatccacag atcaaggata tcttgtcttc
     1501 gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
     1561 cagctgcctt gtaagtcatt ggtcttaaag gtacctgagg tgtgactgga aaacccacct
     1621 cctcctcctc ttgtgcttct agccaggcac aatcagcatt ggtagctgct gtattgctac
     1681 ttgtgattgc tccatgtttt tctaggtctc gaggtcgacg gtatcgatgc ggggaggcgg
     1741 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     1801 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     1861 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga aaggacgatg
     1921 atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     1981 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     2041 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     2101 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     2161 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     2221 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag
     2281 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
     2341 ctagagtcgc ggccgcttag ctatgagcta ctgcatcttc tacctgctca gtaatgttgc
     2401 cttcggtttt cctgacgagg cgatggccga tgtaatggtc tcctggcatt tcaagaatct
     2461 cttttgccgc cttgtaagta gtcttcattt ggcatttgtg attgccgcct ccttcaagtt
     2521 ttaggtacat cgtaacatca cccttcagaa ctccgtcgct ggcagtaatt ttctcggttg
     2581 atggctccca atcaacactt tggttttgca tgataggacc atcggcagga aagttaaccc
     2641 cagtaaattt ggatttgtgg tagaaggtgt ttcctctaag gcttatatgc gcactgactg
     2701 aagcggaccc accatcttcg aactccaacg acctttccca tgacaggcct tcaggaaatg
     2761 cttgtttgaa atagtctggt atctcttctg gatatttagt aaatactctg tggccgtaac
     2821 agaacacgtg tgacactaag tcaaacgcga aaggcattgg cccgccctcg gccattgtga
     2881 cgcgtagtgt catctcttga tgtccctcgt aaggtctgcc tgtgccttca ccttcaattg
     2941 tgaactcatg cccattgacg gagccgtcca tgtagtacct catcttcatc tctggtttaa
     3001 tcacactcac catggtggcg accggtggat cccccgctgc ccccagaact aggggccact
     3061 cgcctgcccg tgctcctgag tgcaaacgga gaaccggctt gactgacgag cttggggctt
     3121 ctgagcaggg cactgtggct gctcacagcc tcgcgagcct tcgtcagcat ccaggtcccc
     3181 atggcaacca cctccgaaac gccctgttcc cgttgccccg gtaactgcct ccccaacacc
     3241 tgcctgcctt tcactttaaa acctgctcct ctttgccctg gccttatata cacaatactg
     3301 caggactgtg tgggcccagg agcaagtgga ccctgttccc ccagggtgat gtactgaggg
     3361 ggttggagaa ggggatgcga acaaacttag tccacaagtg atcatcgctc ttcctacctc
     3421 ccccacctcc aactataggg cccttttaac agacgggatg ggattaagtg gggcaaagag
     3481 gcagttgcta tggtaacggc tagtgggtgc cacattctgg gatggtcctt gagaaagaca
     3541 ggatgtagtt accctggcaa cttcatctcc tctgaagaga caggtattgc cccgttgccc
     3601 tggcaacagc tgggttctaa ggtttacagg ggaagctaat tcatctctcc ttcccttccc
     3661 ccttgtagtc tctgtaatgg tttctttgct tcctctggga attggtctgg ttgacttggc
     3721 ctctgtctct gtccttgttt ctttccagct ctccctgctt tttagccatt gtgcatatca
     3781 caccgccacc ttagcgtgag aagaagtacc aaacagaccc agaatgctga cggaatgact
     3841 ctgtctgctt tctcacccct atctgtcacc actgagcagc agggcccctc ctgaggcctc
     3901 tgccacagct ctccccaccc atcctcccag agggaccatg aagatttggt ctcctggggc
     3961 tcctgtccca gtttctgctt gtgcttcccg tcaaggtagg actggagaga ggcaactata
     4021 atgaggtgaa cgtggctgga ctcacccagg gctgggcggg tggtgtagat gtaccctgcc
     4081 ctggactctt ctttcctaaa ctcattggtc atctgcccta atgagttcac tccttctgta
     4141 tatcgtactt gcatgccccc aaggcatgga atttaagatt agtaatctga gttcaagttc
     4201 tagctctgac atttctgatg tgaccctggg caggtcactg aggaagctct ctgcaccccc
     4261 atctcatttt ctagaaaatc agaagaagaa ccccatggag atgaagtgac ctaaggccat
     4321 aatgttaatt aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     4381 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     4441 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     4501 tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc
     4561 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     4621 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     4681 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     4741 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     4801 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     4861 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     4921 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     4981 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     5041 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     5101 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     5161 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     5221 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     5281 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     5341 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     5401 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     5461 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     5521 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     5581 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     5641 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     5701 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     5761 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     5821 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     5881 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     5941 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     6001 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     6061 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
     6121 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     6181 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     6241 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     6301 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     6361 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     6421 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     6481 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     6541 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     6601 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     6661 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     6721 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     6781 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     6841 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     6901 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9061 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
     9121 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     9181 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     9241 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     9301 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     9361 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg
     9421 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     9481 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     9541 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     9601 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     9661 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     9721 tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt
     9781 atactatgcc gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa
     9841 aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     9901 tcactacttc tggaata
//
