LOCUS       sequence-17401-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Damelin M, Silver PA
  TITLE     Mapping interactions between nuclear transport factors in living 
            cells reveals pathways through the nuclear pore complex.
  JOURNAL   Mol Cell 2000 Jan;5(1):133-40.
  PUBMED    10678175
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2000-01"; volume: "5(1)"; pages: "133-40"
COMMENT     Sequence Label: pPS1890
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(34..50)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(35..51)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(55..57,58..60,61..573)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(361..380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agctatggtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgtaccat cttcttcaag
      361 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      541 aaggcccact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      661 agcacccagt ccgccctg
//
