LOCUS       sequence-174011-        7772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil  peptide SYNZIP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pQLinkHD-SYNZIP1
FEATURES             Location/Qualifiers
     source          1..7772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(347..374)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(466..552)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     601..623
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     615..632
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     616..632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    648..747
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(940..964)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      986..1080
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1174..1833
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1240..1259)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1898..1984
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2077..3159)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3121..3140
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3160..3237)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(3321..3343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3429..3569
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3484..3503
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3755..4343)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4490..5299)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5213..5232
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5291..5310
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     5412..5428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5412..5428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5433..5452
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5433..5451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5469..5568
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5761..5785)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5798..5818)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     RBS             5831..5842
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    5861..5877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5885..5891,5892..5908,5909..5914,
                     5915..5929))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    5893..5909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(5979..5997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6065..6169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6170..6238,6239..7030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6388..6407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7201..7772,1..17)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
       61 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      301 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
      361 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
      421 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
      481 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
      541 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
      601 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
      661 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
      721 tgccaacttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg ggatccaacc
      781 tggttgcgca gctcgaaaac gaagttgcgt ctctggaaaa tgagaacgaa accctgaaga
      841 aaaagaacct gcacaaaaaa gacctgatcg cgtacctgga gaaagaaatc gcgaatctgc
      901 gtaagaaaat cgaagaatga taaaagggtg ggcgcgccga cccagctttc ttgtacaaag
      961 tggtaagctt aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa
     1021 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     1081 ccaagtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taaggctagc
     1141 ttggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1201 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1261 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1381 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1441 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1501 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1561 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     1621 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1681 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1741 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1801 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1861 taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc
     1921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1981 aaatccgccc tctagattac gtgcagtcga tgataagctg tcaaacatga gaattgtgcc
     2041 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2101 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2161 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     2221 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     2281 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     2341 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     2401 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     2461 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     2521 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     2581 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     2641 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     2701 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     2761 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     2821 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     2881 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     2941 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     3001 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     3061 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     3121 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     3181 ctcttccggg cgctatcatg ccataccgcg aaaggttttg caccattcga tggtgtcgga
     3241 atttcgggca gcgttgggtc ctggccacgg gtgcgcatga tctagagctg cctcgcgcgt
     3301 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3361 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3421 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     3481 atgcggcatc agagcagatt gtactgagag tgcaccacat gcggtgtgaa ataccgcaca
     3541 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     3601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3781 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3841 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3901 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3961 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4021 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4081 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4141 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4201 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4261 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4321 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4381 agtggaacga cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc
     4441 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     4501 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     4561 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     4621 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     4681 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     4741 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     4801 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     4861 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     4921 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     4981 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     5041 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     5101 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     5161 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     5221 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt
     5281 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     5341 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
     5401 gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcag
     5461 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
     5521 ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggtcg gcgcgcccac
     5581 ccttttatca ttcttcgatt ttcttacgca gattcgcgat ttctttctcc aggtacgcga
     5641 tcaggtcttt tttgtgcagg ttctttttct tcagggtttc gttctcattt tccagagacg
     5701 caacttcgtt ttcgagctgc gcaaccaggt tggatcccat ggtgaagggg gcggccgcgg
     5761 agcctgcttt tttgtacaaa cttgtacctg cagcaccatg gtgatggtga tggtgatgtt
     5821 tcatagttaa tttctcctct ttaatgaatt ctgtgtgaaa ttgttatccg ctcacaattg
     5881 aatctattat aattgttatc cgctcacaaa gcaaataaat tttttatgat ttctcgacaa
     5941 atggtgttgt taattaagct cgaggtgaag acgaaagggc ctcgtgatac gcctattttt
     6001 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6181 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6241 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6301 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6361 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6421 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6481 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6541 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6601 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6661 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6721 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6781 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6841 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6901 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6961 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7021 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7081 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7321 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7741 tgagcgtcga tttttgtgat gctcgtcagg gg
//
