LOCUS       sequence-174054-        7677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent human androgen receptor (fused to EGFP) with point 
            mutation C619Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Nazareth LV, Stenoien DL, Bingman WE 3rd, James AJ, Wu C, Zhang Y, 
            Edwards DP, Mancini M, Marcelli M, Lamb DJ, Weigel NL
  TITLE     A C619Y mutation in the human androgen receptor causes inactivation 
            and mislocalization of the receptor with concomitant sequestration 
            of SRC-1 (steroid receptor coactivator 1)
  JOURNAL   Mol Endocrinol. 1999 Dec;13(12):2065-75.
  PUBMED    10598582
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Endocrinol."; date: "1999-12"; volume: "13(12)"; pages: "2065-75"
COMMENT     Sequence Label: pEGFP-C1-AR C619Y
FEATURES             Location/Qualifiers
     source          1..7677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1733..1854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1770..1789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1824..1843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1861..2316)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1998..2019)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2210..2229
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2343..2447
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2449..2806
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2449..2640
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2472..2492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2657..2792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2841..3635
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2841..3635
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2895..2914)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3505..3524
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3823..3842)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3867..3914
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4243..4831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4732..4751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5006..5309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5310..5513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5464..5484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5552..5561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5558..5560,5561..5563,5564..6274)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5558..5560,5561..5563,5564..6274)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5603..5624)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5864..5883)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6211..6232
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccctacggct acactcggcc ccctcagggg ctggcgggcc aggaaagcga cttcaccgca
       61 cctgatgtgt ggtaccctgg cggcatggtg agcagagtgc cctatcccag tcccacttgt
      121 gtcaaaagcg aaatgggccc ctggatggat agctactccg gaccttacgg ggacatgcgt
      181 ttggagactg ccagggacca tgttttgccc attgactatt actttccacc ccagaagacc
      241 tgcctgatct gtggagatga agcttctggg tgtcactatg gagctctcac atgtggaagc
      301 tgcaaggtct tcttcaaaag agccgctgaa gggaaacaga agtacctgtg cgccagcaga
      361 aatgattgca ctattgataa attccgaagg aaaaattgtc catcttgtcg tcttcggaaa
      421 tattatgaag caggaatgac tctgggagcc cggaagctga agaaacttgg taatctgaaa
      481 ctacaggagg aaggagaggc ttccagcacc accagcccca ctgaggagac aacccagaag
      541 ctgacagtgt cacacattga aggctatgaa tgtcagccca tctttctgaa tgtcctggaa
      601 gccattgagc caggtgtagt gtgtgctgga cacgacaaca accagcccga ctcctttgca
      661 gccttgctct ctagcctcaa tgaactggga gagagacagc ttgtacacgt ggtcaagtgg
      721 gccaaggcct tgcctggctt ccgcaactta cacgtggacg accagatggc tgtcattcag
      781 tactcctgga tggggctcat ggtgtttgcc atgggctggc gatccttcac caatgtcaac
      841 tccaggatgc tctacttcgc ccctgatctg gttttcaatg agtaccgcat gcacaagtcc
      901 cggatgtaca gccagtgtgt ccgaatgagg cacctctctc aagagtttgg atggctccaa
      961 atcacccccc aggaattcct gtgcatgaaa gcactgctac tcttcagcat tattccagtg
     1021 gatgggctga aaaatcaaaa attctttgat gaacttcgaa tgaactacat caaggaactc
     1081 gatcgtatca ttgcatgcaa aagaaaaaat cccacatcct gctcaagacg cttctaccag
     1141 ctcaccaagc tcctggactc cgtgcagcct attgcgagag agctgcatca gttcactttt
     1201 gacctgctaa tcaagtcaca catggtgagc gtggactttc cggaaatgat ggcagagatc
     1261 atctctgtgc aagtgcccaa gatcctttct gggaaagtca agcccatcta tttccacacc
     1321 cagtgaagca ttggaaaccc tatttcccca ccccagctca tgcccccttt cagatgtctt
     1381 ctgcctgtta taactctgca ctactcctct ctagaggatc ccgggcccca ggcacccaga
     1441 ggccgcgagc gcagcacctc ccggcgccag tttgctgctg ctgcagcagc agcagcagca
     1501 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcaag agactagccc
     1561 caggcagcag cagcagcagc agggtgagga tggttctccc caaggatcca ccggatctag
     1621 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1681 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     1741 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1801 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     1861 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     1921 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     1981 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2041 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2101 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2161 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     2221 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     2281 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     2341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     2461 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2581 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     2761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     2821 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     2881 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     2941 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3001 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     3061 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3121 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3181 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3241 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3301 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3361 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3421 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3481 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3541 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3601 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     3661 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     3721 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     3781 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     3841 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     3901 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     3961 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     4021 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     4081 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4381 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4921 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
     4981 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5041 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5101 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5161 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5221 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5281 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     5341 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5401 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     5461 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     5521 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
     5581 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5641 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5701 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     5761 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5821 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     5881 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     5941 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6001 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6061 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6121 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6181 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6241 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
     6301 ttcgaattct gcagtcgacg gtaccatgga agtgcagtta gggctgggaa gggtctaccc
     6361 tcggccgccg tccaagacct accgaggagc tttccagaat ctgttccaga gcgtgcgcga
     6421 agtgatccag aacccgggcc ccaggcaccc agaggccgcg agcgcagcac ctcccggcgc
     6481 cagtttgctg ctgctgcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     6541 gcagcagcag cagcagcagc aagagactag ccccaggcag cagcagcagc agcagggtga
     6601 ggatggttct ccccaagccc atcgtagagg ccccacaggc tacctggtcc tggatgagga
     6661 acagcaacct tcacagccgc agtcggccct ggagtgccac cccgagagag gttgcgtccc
     6721 agagcctgga gccgccgtgg ccgccagcaa ggggctgccg cagcagctgc cagcacctcc
     6781 ggacgaggat gactcagctg ccccatccac gttgtccctg ctgggcccca ctttccccgg
     6841 cttaagcagc tgctccgctg accttaaaga catcctgagc gaggccagca ccatgcaact
     6901 ccttcagcaa cagcagcagg aagcagtatc cgaaggcagc agcagcggga gagcgaggga
     6961 ggcctcgggg gctcccactt cctccaagga caattactta gggggcactt cgaccatttc
     7021 tgacaacgcc aaggagttgt gtaaggcagt gtcggtgtcc atgggcctgg gtgtggaggc
     7081 gttggagcat ctgagtccag gggaacagct tcggggggat tgcatgtacg ccccactttt
     7141 gggagttcca cccgctgtgc gtcccactcc ttgtgcccca ttggccgaat gcaaaggttc
     7201 tctgctagac gacagcgcag gcaagagcac tgaagatact gctgagtatt cccctttcaa
     7261 gggaggttac accaaagggc tagaaggcga gagcctaggc tgctctggca gcgctgcagc
     7321 agggagctcc gggacacttg aactgccgtc taccctgtct ctctacaagt ccggagcact
     7381 ggacgaggca gctgcgtacc agagtcgcga ctactacaac tttccactgg ctctggccgg
     7441 accgccgccc cctccgccgc ctccccatcc ccacgctcgc atcaagctgg agaacccgct
     7501 ggactacggc agcgcctggg cggctgcggc ggcgcagtgc cgctatgggg acctggcgag
     7561 cctgcatggc gcgggtgcag cgggacccgg ttctgggtca ccctcagccg ccgcttcctc
     7621 atcctggcac actctcttca cagccgaaga aggccagttg tatggaccgt gtggtgg
//
