LOCUS       sequence-174058-       14459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetracyline-inducible lentiviral vector expressing human polybromo-1
            (PBRM1) in mammlian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Chowdhury B, Porter EG, Stewart JC, Ferreira CR, Schipma MJ, 
            Dykhuizen EC
  TITLE     PBRM1 Regulates the Expression of Genes Involved in Metabolism and 
            Cell Adhesion in Renal Clear Cell Carcinoma.
  JOURNAL   PLoS One. 2016 Apr 21;11(4):e0153718. doi: 
            10.1371/journal.pone.0153718. eCollection 2016.
  PUBMED    27100670
REFERENCE   2  (bases 1 to 14459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-21"; volume: "11(4):e0153718. doi"; pages: " 
            10.1371/journal.pone.0153718. eCollection 2016"
COMMENT     Sequence Label: TetO-FUW-PBRM1-pgk-puro
FEATURES             Location/Qualifiers
     source          1..14459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4959..5458
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4994..5017)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5391..5410
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5479..6078
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5479..5498)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5975..5995
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6102..6690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6155..6175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6573..6584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6693..6709)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6694..6710)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7215..7395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7421..7438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7427..7651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7697..8125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7784..7803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7994..8015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8134..8154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8139..8468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8319..8454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8381..8400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8516..8563
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8582..8956
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9086..9207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9123..9142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9177..9196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9256..9272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9256..9272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9269..9291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9280..9296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9304..9310,9311..9328,9329..9334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9349..9370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9487..9504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9658..10246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9738..9757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10417..11208,11209..11277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11040..11059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11278..11382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11457..11476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11648..12027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12029..12227
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12245..12425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12472..12597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13090..13323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13508..13552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13850..13967
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(14024..14042,14043..14065,14066..14084,14085..14107,
                     14108..14126,14127..14149,14150..14168,14169..14191,
                     14192..14210,14211..14233,14234..14252,14253..14275,
                     14276..14294)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        14327..14365
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     14362..14386
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattgctagc cgatggatcc gtgaattcgc caccatgggt tccaagagaa gaagagctac
       61 ctccccttcc agcagtgtca gcggggactt tgatgatggg caccattctg tgtcaacacc
      121 aggcccaagc aggaaaagga ggagactttc caatcttcca actgtagatc ctattgccgt
      181 gtgccatgaa ctctataata ccatccgaga ctataaggat gaacagggca gacttctctg
      241 tgagctcttc attagggcac caaagcgaag aaatcaacca gactattatg aagtggtttc
      301 tcagcccatt gacttgatga aaatccaaca gaaactaaaa atggaagagt atgatgatgt
      361 taatttgctg actgctgact tccagcttct ttttaacaat gcaaagtcct attataagcc
      421 agattctcct gaatataaag ccgcttgcaa actctgggat ttgtaccttc gaacaagaaa
      481 tgagtttgtt cagaaaggag aagcagatga cgaagatgat gatgaagatg ggcaagacaa
      541 tcagggcaca gtgactgaag gatcttctcc agcttacttg aaggagatcc tggagcagct
      601 tcttgaagcc atagttgtag ctacaaatcc atcaggacgt ctcattagcg aactttttca
      661 gaaactgcct tctaaagtgc aatatccaga ttattatgca ataattaagg agcctataga
      721 tctcaagacc attgcccaga ggatacagaa tggaagctac aaaagtattc atgcaatggc
      781 caaagatata gatctcctcg caaaaaatgc caaaacttat aatgagcctg gctctcaagt
      841 attcaaggat gcaaattcaa ttaaaaaaat attttatatg aaaaaggctg aaattgaaca
      901 tcatgaaatg gctaagtcaa gtcttcgaat gaggactcca tccaacttgg ctgcagccag
      961 actgacaggt ccttcacaca gtaaaggcag ccttggtgaa gagagaaatc ccactagcaa
     1021 gtattaccgt aataaaagag cagtacaagg aggtcgttta tcagcaatta caatggcact
     1081 tcaatatggc tcagaaagtg aagaagatgc tgctttagct gctgcacgct atgaagaggg
     1141 agagtcagaa gcagaaagca tcacttcctt tatggatgtt tcaaatcctt tttatcagct
     1201 ttatgacaca gttaggagtt gtcggaataa ccaagggcag ctaatagctg aaccttttta
     1261 ccatttgcct tcaaagaaaa aataccctga ttattaccag caaattaaaa tgcccatatc
     1321 actacaacag atccgaacaa aactgaagaa tcaagaatat gaaactttag atcatttgga
     1381 gtgtgatctg aatttaatgt ttgaaaatgc caaacgctat aatgtgccca attcagccat
     1441 ctacaagcga gttctaaaat tgcagcaagt tatgcaggca aagaagaaag agcttgccag
     1501 gagagacgat atcgaggacg gagacagcat gatctcttca gccacctctg atactggtag
     1561 tgccaaaaga aaaagtaaaa agaacataag aaagcagcga atgaaaatct tattcaatgt
     1621 tgttcttgaa gctcgagagc caggttcagg cagaagactt tgtgacctat ttatggttaa
     1681 accatccaaa aaggactatc ctgattatta taaaatcatc ttggagccaa tggacttgaa
     1741 aataattgag cataacatcc gcaatgacaa atatgctggt gaagagggaa tgatagaaga
     1801 catgaagctg atgttccgga atgccaggca ctataatgag gagggctccc aggtttataa
     1861 tgatgcacat atcctggaga agttactcaa ggagaaaagg aaagagctgg gcccactgcc
     1921 tgatgatgat gacatggctt ctcccaaact caagctgagt aggaagagtg gcatttctcc
     1981 taaaaaatca aaatacatga ctccaatgca gcagaaacta aatgaggtct atgaagctgt
     2041 aaagaactat actgataaga ggggtcgccg cctcagtgcc atatttctga ggcttccctc
     2101 tagatctgag ttgcctgact actatctgac tattaaaaag cccatggaca tggaaaaaat
     2161 tcgaagtcac atgatggcca acaagtacca agatattgac tctatggttg aggactttgt
     2221 catgatgttt aataatgcct gtacgtacaa tgagccggag tctttgatct acaaagatgc
     2281 tcttgttcta cacaaagtcc tgcttgaaac acgcagagac ctggagggag atgaggactc
     2341 tcatgtccca aatgtgactt tgctgattca agagcttatc cacaatcttt ttgtgtcagt
     2401 catgagtcat caggatgatg agggaagatg ctacagcgat tctttagcag aaattcctgc
     2461 tgtggatccc aactttccta acaaaccacc ccttacattt gacataatta ggaagaatgt
     2521 tgaaaataat cgctaccgtc ggcttgattt atttcaagag catatgtttg aagtattgga
     2581 acgagcaaga aggatgaatc ggacagattc agaaatatat gaagatgcag tagaacttca
     2641 gcagtttttt attaaaattc gtgatgaact ctgcaaaaat ggagagattc ttctttcacc
     2701 ggcactcagc tataccacaa aacatttgca taatgatgtg gagaaagaga gaaaggaaaa
     2761 attgccaaaa gaaatagagg aagataaact aaaacgagaa gaagaaaaaa gagaagctga
     2821 aaagagtgaa gattcctctg gtgctgcagg cctctcaggc ttacatcgca catacagcca
     2881 ggactgtagc tttaaaaaca gcatgtacca tgttggagat tacgtctatg tggaacctgc
     2941 agaggccaac ctacaaccac atatcgtctg tattgaaaga ctgtgggagg attcagctgg
     3001 tgaaaaatgg ttgtatggct gttggtttta ccgaccaaat gaaacattcc acctggctac
     3061 acgaaaattt ctagaaaaag aagtttttaa gagtgactat tacaacaaag ttccagttag
     3121 taaaattcta ggcaagtgtg tggtcatgtt tgtcaaggaa tactttaagt tatgcccaga
     3181 aaacttccga gatgaggatg tttttgtctg tgaatcacgg tattctgcca aaaccaaatc
     3241 ttttaagaaa attaaactgt ggaccatgcc catcagctca gtcaggtttg tccctcggga
     3301 tgtgcctctg cctgtggttc gcgtggcctc tgtatttgca aatgcagata aaggtgatga
     3361 tgagaagaat acagacaact cagaggacag tcgagctgaa gacaatttta acttggaaaa
     3421 ggaaaaagaa gatgtccctg tggaaatgtc caatggtgaa ccaggttgcc actactttga
     3481 gcagctccat tacaatgaca tgtggctgaa ggttggcgac tgtgtcttca tcaagtccca
     3541 tggcctggtg cgtcctcgtg tgggcagaat tgaaaaagta tgggttcgag atggagctgc
     3601 atatttttat ggccccatct tcattcaccc tgaagaaaca gagcatgagc ccacaaaaat
     3661 gttctacaaa aaagaagtat ttctgagtaa tctggaagaa acctgcccca tgacatgtat
     3721 tctcggaaag tgtgctgtgt tgtcattcaa ggacttcctc tcctgcaggc caactgaaat
     3781 accagaaaat gacattctgc tttgtgagag ccgctacaat gagagcgaca agcagatgaa
     3841 gaaattcaaa ggattgaaga ggttttcact ctctgctaaa gtggtagatg atgaaattta
     3901 ctacttcaga aaaccaattg ttcctcagaa ggagccatca cctttgctgg aaaagaagat
     3961 ccagttgcta gaagctaaat ttgccgagtt agaaggtgga gatgatgata ttgaagagat
     4021 gggagaagaa gatagtgagg tcattgaacc tccttctcta cctcagcttc agacccccct
     4081 ggccagtgag ctggacctca tgccctacac acccccacag tctaccccaa agtctgccaa
     4141 aggcagtgca aagaaggaag gctccaaacg gaaaatcaac atgagtggct acatcctgtt
     4201 cagcagtgag atgagggctg tgattaaggc ccaacaccca gactactctt tcggggagct
     4261 cagccgcctg gtggggacag aatggagaaa tcttgagaca gccaagaaag cagaatatga
     4321 aggcatgatg ggtggctatc cgccaggcct tccacctttg cagggcccag ttgatggcct
     4381 tgttagcatg ggcagcatgc agccacttca ccctgggggg cctccacccc accatcttcc
     4441 gccaggtgtg cctggcctcc cgggcatccc accaccgggt gtgatgaacc aaggagtggc
     4501 ccctatggta gggactccag caccgggtgg aagtccatat ggacaacagg tgggagtttt
     4561 ggggcctcca gggcagcagg caccacctcc atatcccggc ccacatccag ctggaccccc
     4621 tgtcatacag cagccaacaa cacccatgtt tgtagctccc ccaccaaaga cccagcggct
     4681 tcttcactca gaggcctacc tgaaatacat tgaaggactc agtgcggagt ccaacagcat
     4741 tagcaagtgg gatcagacac tggcagctcg aagacgcgac gtccatttgt cgaaagaaca
     4801 ggagagccgc ctaccctctc actggctgaa aagcaaaggg gcccacacca ccatggcaga
     4861 tgccctctgg cgccttcgag atttgatgct ccgggacacc ctcaacattc gccaagcata
     4921 caacctagaa aatgtttaag aattccatac cggttaccgg gtaggggagg cgcttttccc
     4981 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     5041 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5101 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5161 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     5221 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     5281 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     5341 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     5401 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     5461 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5521 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5581 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5641 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5701 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5761 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5821 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5881 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5941 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6001 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6061 cgcaagcccg gtgcctgaaa ttcgatatca agcttatcga taatcaacct ctggattaca
     6121 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6181 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6241 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6301 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6361 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6421 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6481 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     6541 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6601 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6661 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     6721 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6781 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6841 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6901 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6961 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     7021 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     7081 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7141 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     7201 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7261 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7321 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7381 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     7441 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7501 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7561 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7621 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     7681 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7741 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7801 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7861 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7921 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7981 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8041 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8101 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     8161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8401 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8461 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     8521 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     8581 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     8641 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     8701 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     8761 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     8821 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     8881 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     8941 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     9001 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     9061 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     9121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9181 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     9241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11401 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    11461 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    11521 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    11581 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    11641 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
    11701 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
    11761 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
    11821 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
    11881 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    11941 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    12001 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
    12061 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
    12121 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
    12181 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
    12241 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    12301 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    12361 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    12421 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    12481 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    12541 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    12601 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    12661 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    12721 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    12781 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    12841 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    12901 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
    12961 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    13021 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    13081 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    13141 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    13201 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    13261 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    13321 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    13381 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    13441 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    13501 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    13561 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    13621 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    13681 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
    13741 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    13801 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
    13861 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    13921 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    13981 gggacagcag agatccagtt tggttagatc tcgagtttac cactccctat cagtgataga
    14041 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
    14101 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
    14161 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
    14221 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
    14281 atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg
    14341 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
    14401 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccg
//
