LOCUS       sequence-174063-        5056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Setd7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Kofent J, Zhang J, Spagnoli FM
  TITLE     The histone methyltransferase Setd7 promotes pancreatic progenitor 
            identity.
  JOURNAL   Development. 2016 Oct 1;143(19):3573-3581. Epub 2016 Aug 30.
  PUBMED    27578787
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-1"; volume: "143(19)"; pages: 
            "3573-3581. Epub 2016 Aug 30"
COMMENT     Sequence Label: x.Setd7
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        662..680
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     662..679
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1942..2076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1966..1985)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2020..2039
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2193..2213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2193..2211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2232..2248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2232..2248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2245..2267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2256..2272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2280..2286,2287..2304,2305..2310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2325..2346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2463..2480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2634..3222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2714..2733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3393..4184,4185..4253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4016..4035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4254..4358)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4384..4839)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4521..4542)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4733..4752
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattggccag gttcaatact atgtattggc cctatgccat atagtattcc atatatgggt
       61 tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat atatggggct
      121 tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata tatggtcttt
      181 cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc attgacgtca
      241 attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac cacccaccat
      301 tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc ctattgacgt
      361 caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt aacttggcaa
      421 gtacattact attggaagta cgccagggta cattggcagt actcccattg acgtcaatgg
      481 cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg gggaggggca
      541 atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta atgggaggtc
      601 tatataagca atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt
      661 gatttaggtg acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc
      721 gaattcgtcg acaggccaat ctggccgcgg ccgcgggaat tcgattccca tggacagtga
      781 ggatgaaaca gtggaggaga cggtagacgg tcttttggat gatgatggat tacctaacgg
      841 gttttgcact gtcaactaca gctcgactga tcggtttgag gggcactttg tacatggaga
      901 aaaaaatgga cgtggaaagt tctacttttt tgatggaagc acattggaag gtttttatgt
      961 tgatgatgca cttcaaggcc aagggattta cacctatgaa gatggaggct cgcttcatgg
     1021 cacttatgtg gaaggggagc taaatggtcc agctcaggaa tatgacaccg acagaaaact
     1081 catcttcaaa ggacaataca aagataatgt ccggcatggc gtatgctgga tatattatcc
     1141 agatggcggt agcctagtgg gtgaggtgaa tgaagatgga gacatgacag gagataagat
     1201 cgcttatgtc tacccagatg gtagaactgc actttatggg aagtttattg attctgaaat
     1261 gctcgaaggg aaattggcaa ttcttacttc agttgatgaa ggaaagcccc actttgacct
     1321 ggttccaagt ggaacagttt atacttttga taaatcaact ccctcctgta tttcaataaa
     1381 tcctctgctc ccagacccat atgaatcaga aagagtatat gttgatgtct ctttgataca
     1441 caatgctgga gaaggtctct ttgctaaggt tgtgtcagca gctcagactg taatgtcatt
     1501 ttataatggt gtacggatta cacatcaaga ggtggatagc agagaatggg ccctaaatgg
     1561 aaacacaatc tctctagatg atgaaacagt ccttgatgtg ccagcaccat acaactcaaa
     1621 taacaaatat tgtgcatctc tgggacacaa ggcaaaccat tccttcacac ccaactgtat
     1681 gtatgatact tttgtccatc ctcgctttgg acccatcaag tgcatccgaa ctattaaagc
     1741 tgtggaaaag gaggaagaac tcaccgtggc ctatggatat gaccataaca tcacagggaa
     1801 aaatggccca gaggcacccg aatggtacca gcagcagctg atggaatttc aagctactca
     1861 acaaaagtga gcgaatcact agtgaattcg cggccgcaag gcctctcgag cctctagaac
     1921 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
     1981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2101 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggcgcg ccgcggcgcc
     2161 aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     2221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2281 aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4381 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4441 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4501 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4561 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4621 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4681 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4741 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4801 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     4861 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgatcgacc
     4921 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
     4981 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
     5041 gccaattcaa tatggc
//
