LOCUS       sequence-174064-        4916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of hdac8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4916)
  TITLE     Spagnoli lab plasmids
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: x.hdac8-ORF
FEATURES             Location/Qualifiers
     source          1..4916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        662..680
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     662..679
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1802..1936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1826..1845)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1880..1899
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2053..2073)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2053..2071)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2092..2108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2105..2127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2116..2132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2140..2146,2147..2164,2165..2170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2185..2206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2323..2340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2494..3082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2574..2593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3253..4044,4045..4113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3876..3895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4114..4218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4244..4699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4381..4402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4593..4612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattggccag gttcaatact atgtattggc cctatgccat atagtattcc atatatgggt
       61 tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat atatggggct
      121 tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata tatggtcttt
      181 cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc attgacgtca
      241 attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac cacccaccat
      301 tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc ctattgacgt
      361 caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt aacttggcaa
      421 gtacattact attggaagta cgccagggta cattggcagt actcccattg acgtcaatgg
      481 cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg gggaggggca
      541 atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta atgggaggtc
      601 tatataagca atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt
      661 gatttaggtg acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc
      721 gaattcgtcg acatggtgca ctcattaatc gaggcatatg ggctgctcaa agagatgagg
      781 gtagtaaagc caaaagttgc atctatggaa gagatggctg cctttcacac tgatgcatat
      841 ctccagcatc tacataaagt cagtgaggaa ggcgacaatg atgatcctga aacacttgaa
      901 tatggactag gttacgactg ccccattact gaaggaatat atgactatgc tgctgctgtg
      961 gggggagcca ctttaactgc tgctgaacag cttatagagg gcaaaactcg cattgctgtc
     1021 aactggcctg gtggttggca tcacgcaaag aaagatgaag cttctggatt ctgttacctt
     1081 aatgatgctg tcctgggtat tttgaaactg cgggaaaaat ttgaccgggt tttgtacgta
     1141 gatatggatt tacatcatgg tgatggggtt gaagacgctt ttagcttcac ttcaaaagta
     1201 atgactgttt ctctccataa attttcccct ggattttttc ctggcactgg tgatgtaagt
     1261 gacattggcc tgggtaaggg acgttactac agtattaatg tgccattaca agatggaata
     1321 caggatgata aatattacca aatctgcgaa ggtgtcctaa aagaggtttt cacaactttt
     1381 aaccctgagg cagttgtcct gcagttggga gcagatacaa tagccgggga tcccatgtgt
     1441 tcttttaaca tgaccccaga ggggattgga aagtgcctaa aatatgttct acagtggcag
     1501 cttccaacac tcatactggg aggagggggc taccatctcc caaacacagc tcgttgctgg
     1561 acatacttga ccgcacttat agttggcagg actttatctt ctgaaatccc agaccacgag
     1621 ttcttcacag aatacggacc tgactatgta ttagagatta caccgagctg tcggcctgac
     1681 cgcaatgaca ctcagaaggt tcaggaaatc ttacaatcaa ttaaaggtaa cttgaaacga
     1741 gtggtttaag cggccgcaag gcctctcgag cctctagaac tatagtgagt cgtattacgt
     1801 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1861 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1921 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1981 ggtgtgggag gttttttaat tcgcggcgcg ccgcggcgcc aatgcattgg gcccggtacc
     2041 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat
     2161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     4261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4681 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     4741 tcggtgcggg cctcttcgct attacgccag tcgatcgacc gccaattcaa tatggcgtat
     4801 atggactcat gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat
     4861 atggactcgt gccaattcaa tatggtggat ctggacccca gccaattcaa tatggc
//
