LOCUS       sequence-174068-        9385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Stard13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9385)
  TITLE     Spagnoli lab plasmids
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mStard13
FEATURES             Location/Qualifiers
     source          1..9385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        652..670
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     652..669
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    6271..6405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6295..6314)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6349..6368
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6522..6542)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6522..6540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6561..6577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6561..6577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6574..6596)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6585..6601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6609..6615,6616..6633,6634..6639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6654..6675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6792..6809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6963..7551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7043..7062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7722..8513,8514..8582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8345..8364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8583..8687)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8713..9168)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8850..8871)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9062..9081
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gttcaatact atgtattggc cctatgccat atagtattcc atatatgggt tttcctattg
       61 acgtagatag cccctcccaa tgggcggtcc catataccat atatggggct tcctaatacc
      121 gcccatagcc actcccccat tgacgtcaat ggtctctata tatggtcttt cctattgacg
      181 tcatatgggc ggtcctattg acgtatatgg cgcctccccc attgacgtca attacggtaa
      241 atggcccgcc tggctcaatg cccattgacg tcaataggac cacccaccat tgacgtcaat
      301 gggatggctc attgcccatt catatccgtt ctcacgcccc ctattgacgt caatgacggt
      361 aaatggccca cttggcagta catcaatatc tattaatagt aacttggcaa gtacattact
      421 attggaagta cgccagggta cattggcagt actcccattg acgtcaatgg cggtaaatgg
      481 cccgcgatgg ctgccaagta catccccatt gacgtcaatg gggaggggca atgacgcaaa
      541 tgggcgttcc attgacgtaa atgggcggta ggcgtgccta atgggaggtc tatataagca
      601 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
      661 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc gaattcgtcg
      721 acccacgcgt ccgcgctggg tctgtggctc tctcctgggg ctccattgac tgaggctgga
      781 gacagctgcg aagagccacc ggcagctgag gtggcagtgg aaactctccc gagatgttca
      841 gccaggtgcc caggacgccg gctgcaggct gctactatct aaaccccttg acacctgaga
      901 gccaggagat gtacttgcga tttgatcaga ctgccagacg ctctccctac aggatgagcc
      961 ggatcctagc acgccatcac ctagtgacta aaatccagca agaaattgag gcgaaggaag
     1021 catgtgactg gctccgagct gccgggttcc cgcagtacgc tcagctgtat gaagattcgc
     1081 agttccccat caacattgcg gccgtcaaaa aagaccatga ctttcttgaa agggaccttg
     1141 tagaacctct ttgcagacga cttaatacgt tgaacaagtg tgcctcaatg agacttgatg
     1201 tgaacttcca aaggaaaaag ggtgacgact cagatgagga agacctgtgc atcagcaaca
     1261 aatggacttt ccagagaacc agccgcagat ggtcccgtgt ggatgacctg catacgctgt
     1321 tccccgtggc agacagaaac gggtccccag gaggccctag gatgagaaac acagccagca
     1381 gtgagagcgt gctcacggat ctgagtgagc cagaggtctg ctctattcac agcgaaagca
     1441 gtgggggcag tgacagccgc agccaatcag ggcatcattc tgctgacagt acgcatgcgc
     1501 tggaggccac cctggtcagt agcagcctcc cacagtctac ccgagagggt ctcaaccagt
     1561 cttttcaccc caagaatgag aaacccacca ggaccagggc caagtccttt ctgaaacgca
     1621 tggataccct gagagtgaag ggtgcacttg ggaggcataa ggggccaggg cggacaggag
     1681 gcctggtcat cagtaggcct gtgctgcagc aggaaccgga gtcctttaag accatgcagt
     1741 gcgtccagat acccaacgga gatctgcaga cctcacctcc agctgcctgc aggaaaggcc
     1801 tcccatgctc cagtaagtca agtggtgaga gcagccccct ggagaacagc agcacagtga
     1861 gcactccgtg catgaaggaa cgcaagtgcc accacgaggc caacaagcgc ggtggcatgt
     1921 acctggagga cctggatgtg ctggcaggga cggcattacc agatacgtca gaccaaaacc
     1981 acgtgcatgg gtttcactcc caagaaaact tggtggtcca cattcccaag gatcacaaac
     2041 caggaacgtt ccccaaggca ctttctatag aaagcctctc acccacagac aacagcaatg
     2101 gggttaactg gaggaccggg agtatctccc tgggtaggca acagggccct ggcatgaggg
     2161 aacccagact catgtcttcc tgccacaggg ccagccgtgt cagtatctat gacaatgtcc
     2221 ccagctcgca cctgtatgcc agcacaggag atctgttgga cctggagaaa gacggcctcc
     2281 ttccacagct ggatgacatc ctacagcatg tcaatggcat acaagaggta gtggatgact
     2341 ggtcaaaaaa catcttaccc gaactgcaaa gtcacagcac attggcaggg gatcctggtc
     2401 tgtccccatt cccatctccc aatcaggtca ctttagattt tgaaggcaac tctgtctcag
     2461 aaggtcggac aacacctagt gatgtggaaa gggacaggac ttctctgaat gaatcggagg
     2521 ccactggggt cagagaaaga agggattctg gtgtgggggc ctctctgacc agaccaaaca
     2581 gacgactaag gtggagcagc ttccagctct cacaccagcc ccagccgtct ccagccaccc
     2641 cgcacatcag cagccagacg gccgcccagc tgaacctgct ccagcgcttc tccctacttc
     2701 gtcttacggc catcatggag aagtactcta tgtccaacaa gcacggctgg acctggtccg
     2761 ttccaaagtt catgaagagg atcaaagctc ctgactaccg agacaaggct gtcttcggtg
     2821 ttccgctcat agtccacgtt cagagaacag gacagcccct gcctcagagc atccaacaag
     2881 cactgaggta tctacgtagc aactgtctgg atcaggtggg tcttttccgc aagtcaggag
     2941 tgaagtctcg aatccatgcc ctccgtcaga tgaatgagaa cttccctgac aatgtgagct
     3001 atgaagacca gtctgcatac gacgtggcag atatggtgaa gcagttcttc cgggacctcc
     3061 ctgagcccct gttcaccaac aagctcagcg agaccttcct ccacatctat cagtatgtcc
     3121 ccaaagagca gcggctgcag gccgtgcagg cagccatcct gctgctggcc gacgagaacc
     3181 gggaggtcct gcagactctc ctgtgcttcc tgcatgacgt agtaaacttg gtggacgaga
     3241 atcagatgac gcccatgaat ctggccgtgt gcctggcccc ctccctcttt cacctaaact
     3301 tactgaagaa agaaagctcc ccaaaagtca tccagaagaa atacgccact gggaagccag
     3361 atcagaagga cctcaatgaa aatctagctg cggctcaggg gctggcccac atgatcacgg
     3421 aatgcaaccg actctttgag gttccacacg agatggtggc ccagtctcgg gactcctact
     3481 tagaggctga aatccatgtg cccagcctgg aagacttggg agcacagctg gcggagagcg
     3541 gggcaacttt tcacacgtac ttggagcatc tagtccaagg cctccagaaa gaggctaagg
     3601 agaaattcaa gggatgggtc acatgttcca gccctgacaa cacagacctt gctttcaaaa
     3661 aggtgggcga tgggcacccg ctgaagctgt ggaaagcatc tgtggaggtg gaggcaccac
     3721 cctccgtggt gctgaaccgt gtgctgagag aacgtcacct gtgggatgag gactttgtgc
     3781 agtggaaggt ggtggaaaga ttggacaaac aaacggaaat ctaccagtac gtgctaaaca
     3841 gcatggttcc acatccctcc agagacttcc tggtgctcag gacctggaag actgacctgc
     3901 ccaaaggaat gtgcaccctg gtgtccctgt ctgtggagta cgaagaagcc cagctcatgg
     3961 gtggcgtgag ggcggtggtg atggactctc agtacctgat agaaccctgc ggttctggca
     4021 agtccaggct gacccacatc tgcagaatag acctgaaggg ccactcccca gaatggtaca
     4081 gcaaaggctt tggacacctc tgtgcggcag aagttaccag aattaggaac tctttccagc
     4141 ctctcgttgc tgagggtcca gaaacaaaaa tctaagcttt ccccagcatg acatcaaact
     4201 cagggaagaa gaagggaaag aacactcaca gcgtggcaag tgcacgcatg tgcaggaatg
     4261 tcgcagtggg atccggagat cgcggagacc gtggagcccg ttcctagcct gcctagagat
     4321 ggctaatccc tactaatata atgtctcgga acttttatct atactgctta aaattaaatg
     4381 ggttatttct tccgcgttgc ctaacttgct atttaaaggc ttctaagaag cgtgtcttag
     4441 ctgtaaataa atgtactgta gcatttgtac atgtatgtat atctcccttt actgtatata
     4501 tgtaaaatac cagttttata tataattcag tgtctgactc tgtgagtctc ctcacatggc
     4561 acttcccagg ttgctgtaga ccccgtgtcc catcccgacc tgtcccgcca gctgagcaga
     4621 gcctgctgac aaacaccaaa gtgtgaaatg tgtagtctgt cagcctgccc aggcttaccc
     4681 aaagattagc taaccaaaga aaaatagagt tgagagcttc ttccacaggt tggcctctta
     4741 tttctgatgt ttaaagactg gagcaggatg ttgacataaa ttgagggata taatatttgc
     4801 ttctctttaa cgcaaagcag caggtgagag gttattgcca aatccaaggc tagaccttgc
     4861 attgcctctg ggccctcttg agattcagtt ctcatagtgc tttttaacca tctatttgac
     4921 cagtggccta tgtggccaca gtttttttaa catgttcttg cactgacctt gactctggac
     4981 accagagtga gatgacagtc catcgagtgt ccaggagtca tcacagcttc agggaattcc
     5041 acacatgcta tagatgatgc ttgtaaaatc taagccatta aaaatcttgc aatctaaaag
     5101 acaggatttt aaacaaaaga ccaaactgta tcattagttt ttgttaccaa tcccatgttt
     5161 cttcattacc cagcaccagc acctgggcgt tcgaccccaa ccttatttat tattgtatag
     5221 actgagcaaa ctggcttccc ttaaccaagt gaggatggag ttgttatttt tttttttatt
     5281 gtgatacaac atctgtagag agataactta gtatgtctgg cctccaggaa agattgtctt
     5341 aggttgtcta aacagagatt gatatcctga ttcagcaact ggtgtcggaa gacgaggaaa
     5401 aaaccaaacc tatatttccc tggtttttgt tgttgttgtg ttatatactt ttatctttta
     5461 tctgtggtgt gcaattgttt tattgggagg aataaagatg ttttctgtat acttggtata
     5521 gcacagggaa aaccagggta agagtttttc attctcgtgt ctacctggga gaggggcagt
     5581 cagaccacat ggtgcctcct gggcagcgtt ctgtggagtg gacaagttca cttctgccat
     5641 tacctcatgc ctaccatgct ggatggcttt gtatgtcttg tcaggaccat cagtggagct
     5701 tagtgtggtt ccacaccatc tgtctctgtg ccagagccgg gtcactgtgc acaggaaaac
     5761 agatcgggcg cccggggatg gctgggttgt ttgtctcggg gcacacttgt ttggcataca
     5821 ctgggaatca actaaagcct ttgttaacag gctcccatcc aggccatgtg ggtgggcctg
     5881 gcgttctgga tttctaatga gcctgaggga tgcagggtgc ctcaaggaca cactttgaga
     5941 aacaaggacc taggaaatac taaaactcaa taaaatatct gatacagtta tattccccag
     6001 cacctttctc ttccaactta gctggctttc tgattgtatg cacatactaa tcatatcact
     6061 tctgctaacc acagggcgcg tctttaaaat aaaggggagg ggagggggag atgatgcatt
     6121 gagcttgttt gtcaaatacc acagtatttt attcattgtt atcttgctga tggaaataaa
     6181 aaaattgcat ttaaatatta aaaaaaaaaa aaaaaagggc ggccgcaagg cctctcgagc
     6241 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     6301 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     6361 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     6421 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggcgcgc
     6481 cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     6541 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6601 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
     6661 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6721 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7381 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7681 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7741 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7801 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7861 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7921 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7981 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8041 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8101 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8161 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8221 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8281 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8341 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8401 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8461 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8521 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8581 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8641 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8701 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     8761 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     8821 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     8881 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     8941 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     9001 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     9061 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     9121 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     9181 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
     9241 cgatcgaccg ccaattcaat atggcgtata tggactcatg ccaattcaat atggtggatc
     9301 tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat atggtggatc
     9361 tggaccccag ccaattcaat atggc
//
