LOCUS       sequence-174073-        5033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of hdac8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5033)
  TITLE     Spagnoli lab plasmids
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: m.hdac8-ORF
FEATURES             Location/Qualifiers
     source          1..5033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        662..680
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     662..679
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1919..2053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1943..1962)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1997..2016
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2170..2190)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2170..2188)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2209..2225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2209..2225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2222..2244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2233..2249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2257..2263,2264..2281,2282..2287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2302..2323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2440..2457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2611..3199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2691..2710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3370..4161,4162..4230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3993..4012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4231..4335)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4361..4816)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4498..4519)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4710..4729
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattggccag gttcaatact atgtattggc cctatgccat atagtattcc atatatgggt
       61 tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat atatggggct
      121 tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata tatggtcttt
      181 cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc attgacgtca
      241 attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac cacccaccat
      301 tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc ctattgacgt
      361 caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt aacttggcaa
      421 gtacattact attggaagta cgccagggta cattggcagt actcccattg acgtcaatgg
      481 cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg gggaggggca
      541 atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta atgggaggtc
      601 tatataagca atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt
      661 gatttaggtg acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc
      721 gaattcgtcg acatggagat gccagaggaa cccgccaaca gtgggcattc actacccccg
      781 gtttatattt acagtcccga gtatgtcagc atctgcgact cccttgtgaa ggtccccaaa
      841 cgggccagta tggtccactc tctgatcgaa gcctatgccc tgcataaaca aatgaggata
      901 gtgaagccca aagtggcctc catggaggag atggccacct tccacactga tgcctatctg
      961 caacatctcc agaaagtcag ccaagaaggt gatgaggacc atccagactc catagaatat
     1021 ggactaggtt atgactgccc agccacagaa gggatatttg actatgcagc agctatagga
     1081 ggaggtacaa tcacagctgc ccagtgcctg attgacggga agtgtaaagt agccatcaac
     1141 tggtctggag ggtggcatca tgcaaagaaa gatgaagcat ctggtttctg ttatctcaat
     1201 gatgctgtcc tgggaatatt acgattgcga cggaaatttg accgtattct ctacgtggat
     1261 ttggatctac accatggaga tggtgtagaa gatgccttca gttttacatc gaaggttatg
     1321 actgtgtccc tgcacaagtt ctccccagga tttttcccag gaacaggtga catgtctgat
     1381 gttggcctgg ggaaaggacg gtactacagt gtcaatgtgc ccattcagga tggcatccag
     1441 gatgagaagt actatcacat ctgtgaaagt gtactaaaag aagtgtacca ggccttcaat
     1501 ccgaaggcag tggttttaca gctgggagca gatactattg ccggagatcc aatgtgttcc
     1561 tttaatatga caccagtggg gatcggcaag tgtctgaagt atgtcctaca gtggcagctg
     1621 gcaactctga ttttaggagg aggaggctat aaccttgcca acacggctcg ttgctggaca
     1681 tacttgaccg gggtcatcct agggaaaacg ctatcctctg agatcccaga tcatgagttt
     1741 ttcacagcat atggtcctga ttatgtgctg gaaatcacgc caagctgccg gccagaccgc
     1801 aatgagcccc accgaatcca gcaaatcctc aactacatca aagggaatct gaagcatgtg
     1861 gtctaggcgg ccgcaaggcc tctcgagcct ctagaactat agtgagtcgt attacgtaga
     1921 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1981 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2041 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2101 gtgggaggtt ttttaattcg cggcgcgccg cggcgccaat gcattgggcc cggtacccag
     2161 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     2221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     2281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2341 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3661 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3961 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     4381 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4441 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4501 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4561 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     4621 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4681 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4741 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4801 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4861 gtgcgggcct cttcgctatt acgccagtcg atcgaccgcc aattcaatat ggcgtatatg
     4921 gactcatgcc aattcaatat ggtggatctg gacctgtgcc aattcaatat ggcgtatatg
     4981 gactcgtgcc aattcaatat ggtggatctg gaccccagcc aattcaatat ggc
//
