LOCUS       sequence-174093-        7470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a CMV promoter when no GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle-CMV
FEATURES             Location/Qualifiers
     source          1..7470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..402)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(84..105)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     296..315
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   440..542
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     630..780
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        838..1141
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1142..1345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1296..1316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1556..1677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1593..1612)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1647..1666
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4868..4970
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(5059..5076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5230..5818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5310..5329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             6650..7444
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6650..7444
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6704..6723)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7314..7333
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
       61 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      121 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      181 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      241 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      301 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      361 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
      421 cgaattaatt cttaattaac atcatcaata atatacctta ttttggattg aagccaatat
      481 gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt
      541 agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt
      601 ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg
      661 ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg
      721 ggaaaactga ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat
      781 actgtaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      841 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      901 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      961 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1021 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1081 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1141 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1201 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1261 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1321 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagatctgg
     1381 taccgtcgac gcggccgctc gagcctaagc ttctagataa gatatccgat ccaccggatc
     1441 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1501 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     1561 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1621 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     1681 cggatctggg cgtggttaag ggtgggaaag aatatataag gtgggggtct tatgtagttt
     1741 tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt
     1801 gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg
     1861 ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag
     1921 accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc
     1981 accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct
     2041 tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg
     2101 acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc
     2161 ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc agactctgtt
     2221 tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg cgcgcggtag
     2281 gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa
     2341 aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg gaggtagcac
     2401 cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta gcaggagcgc
     2461 tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg caggcccttg
     2521 gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga tatgagatgc
     2581 atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg gggattcatg
     2641 ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc atgtagctta
     2701 gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt ttccatgcat
     2761 tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat atttctggga
     2821 tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt tacaaagcgc
     2881 gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc gtagttaccc
     2941 tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc tacctgcggg
     3001 gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag caggttcctg
     3061 agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac cggctgcaac
     3121 tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac ttcgttaagc
     3181 atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc gccgcccagc
     3241 gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc
     3301 atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt cacctgctct
     3361 acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg
     3421 gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg cgcagggtcc
     3481 tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg
     3541 tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg
     3601 ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc
     3661 gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg agggcgtaga
     3721 gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag gccccgcaga
     3781 cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa accaggtttc
     3841 ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt ccacgctcgg
     3901 tgacgaaaag gctgtccgtg tccccgtata cagacttgag agggagttta aacgaattca
     3961 atagcttgtt gcatgggcgg cgatataaaa tgcaaggtgc tgctcaaaaa atcaggcaaa
     4021 gcctcgcgca aaaaagaaag cacatcgtag tcatgctcat gcagataaag gcaggtaagc
     4081 tccggaacca ccacagaaaa agacaccatt tttctctcaa acatgtctgc gggtttctgc
     4141 ataaacacaa aataaaataa caaaaaaaca tttaaacatt agaagcctgt cttacaacag
     4201 gaaaaacaac ccttataagc ataagacgga ctacggccat gccggcgtga ccgtaaaaaa
     4261 actggtcacc gtgattaaaa agcaccaccg acagctcctc ggtcatgtcc ggagtcataa
     4321 tgtaagactc ggtaaacaca tcaggttgat tcacatcggt cagtgctaaa aagcgaccga
     4381 aatagcccgg gggaatacat acccgcaggc gtagagacaa cattacagcc cccataggag
     4441 gtataacaaa attaatagga gagaaaaaca cataaacacc tgaaaaaccc tcctgcctag
     4501 gcaaaatagc accctcccgc tccagaacaa catacagcgc ttccacagcg gcagccataa
     4561 cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg acacggcacc
     4621 agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat ataggactaa
     4681 aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg cgaacctacg
     4741 cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc gttttcccac
     4801 gttacgtcac ttcccatttt aagaaaacta caattcccaa cacatacaag ttactccgcc
     4861 ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa actccacccc
     4921 ctcattatca tattggcttc aatccaaaat aaggtatatt attgatgatg ttaattaaca
     4981 tgcatggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5041 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5101 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5641 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6301 tgctgcagcc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc
     6361 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     6421 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     6481 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     6541 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     6601 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     6661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     6841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     7021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7141 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     7201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7441 ctgaattttg ttaaaatttt tgttaaatca
//
