LOCUS       sequence-174098-        8923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-mPar6a K19A - Does not bind to aPKC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Nolan ME, Aranda V, Lee S, Lakshmi B, Basu S, Allred DC, Muthuswamy 
            SK.
  TITLE     The polarity protein Par6 induces cell proliferation and is 
            overexpressed in breast cancer.
  JOURNAL   Cancer Res. 2008 Oct 15;68(20):8201-9.
  PUBMED    18922891
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2008-10-15"; volume: "68(20)"; pages: "8201-9"
COMMENT     Sequence Label: FLAG-mPar6a K19A
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             135..734
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(135..154)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     631..651
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    795..1362
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    812..1364
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(956..973)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1183..1202
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1376..1378,1379..1381,1382..2095)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1421..1442)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1682..1701)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2029..2050
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2197..2711
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2873..2889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2897..2903,2904..2921,2922..2927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2942..2963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3080..3097)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3251..3839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3331..3350)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4010..4801,4802..4870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4633..4652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4871..4975)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5043..5061
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5099..5121)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5221..5240
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5434..5456
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5584..5603
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5803..6319
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6383..6724
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             6791..7207
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7137..7159
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7175..7191
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7262..7285
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8586..8609)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
       61 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      121 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      181 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      241 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      301 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      361 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      421 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      481 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      541 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
      601 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
      661 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
      721 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
      781 cccatgcatc gaattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa
      841 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
      901 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      961 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     1021 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     1081 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     1141 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     1201 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     1261 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     1321 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     1381 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1441 cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa
     1501 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1561 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1621 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1681 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1741 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1801 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1861 caaggcgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1921 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1981 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2041 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaatgaat
     2101 taattaagaa ttatcaagct tatcgatacc gtcgacctgc agccaagctt atcgataaaa
     2161 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     2221 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag
     2281 agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc
     2341 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     2401 ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     2461 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     2521 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
     2581 gatagactgc gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     2641 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     2701 gggtctttca tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc
     2761 gaatattaag taatcctgac tcaattagcc actgttttga atccacatac tccaatactc
     2821 ctgaaatagt tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc
     2881 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2941 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3001 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3061 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3241 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3661 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4141 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4981 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     5041 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     5101 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5161 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     5221 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5281 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     5341 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     5401 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     5461 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     5521 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     5581 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     5641 cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt
     5701 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
     5761 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     5821 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
     5881 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
     5941 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     6001 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     6061 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     6121 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
     6181 gtcctccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt
     6241 gcatccgaat cgtggactcg ctgatccttg ggagggtctc ctcagattga ttgactgccc
     6301 acctcggggg tctttcattt ggaggttcca ccgagatttg gagacccctg cccagggacc
     6361 accgaccccc ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg tctctgtctt
     6421 tgtgcgtgtt tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt tagctaacta
     6481 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
     6541 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
     6601 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     6661 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     6721 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     6781 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     6841 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     6901 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     6961 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     7021 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     7081 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     7141 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     7201 aggcgccgga attagatctc tcgaggttcg tttagtgaac cgtcagaatt gatctaccat
     7261 ggactacaaa gacgatgacg acaagcttgc ggccgcgaat tccatggcca ggccgcagag
     7321 gactccggcg cgcagtcccg atagcatcgt cgaggtggcg agcaaatttg acgccgagtt
     7381 ccgacgcttt gcactacccc gcacttcggt gagaggcttt caggagttct cgcgattgct
     7441 gtgtgtggta caccagatcc ctggcctgga cgtcctgctt ggctatacgg atgctcacgg
     7501 tgacttgctg cccctcacca acgatgacag tttgcaccgg gccctggcca gcgggccccc
     7561 acctctgcgc ctcttggttc agaaacgggc agaaggtgac tcgagtggcc tggcttttgc
     7621 ctccaactct ctacaaaggc gcaagaaagg gctcctgcta cgaccagtgg cacctctgcg
     7681 caccaggcca cccttgttaa tcagcttgcc ccaagatttc cgccaggtgt cttcagttat
     7741 agatgtggac ctactacctg agacccaccg acgagtgagg ctgcacaaac atggttcaga
     7801 ccgtcccctg ggcttctaca ttcgagatgg catgagtgtt cgcgtggctc cccagggcct
     7861 ggagcgggtt ccaggtatct tcatctcccg cctggtacgt gggggcctgg ctgagagtac
     7921 agggctgctg gcggtcagtg atgagatcct tgaggtcaac ggcattgagg tggccgggaa
     7981 gaccttggac caagtgacgg acatgatggt cgccaacagc cacaacctca tcgtcactgt
     8041 caagcctgcc aaccagcgta ataatgtggt acggggggca tctgggcgtc tgacagggcc
     8101 ttcctctgta gggcctgggc ctactgatcc tgacagtgac gatgacagca gtgacctggt
     8161 cattgagaat cgccaccctc cctgttctaa tgggctgtct caggggcccc tgtgctggga
     8221 cctgcaacct ggctgcctac ttcctggtgc tggcagctct ctgccctcct tggatagcag
     8281 agagcaagcc aattctggct gggggaatgg catgcgaggt gatgttagcg gattcagcct
     8341 ctgacaggca aagatgcagc ccttgtcact ttaggctgcg ggaacatggc ctggactttc
     8401 cagctgggta gagaagatta gcttgctcac agagccctct cagtctggtg aacattaaag
     8461 gttttctaca aatgtaaaaa aaaaaaaaaa aaaaaaaaat ttccgcggcc atcggtacca
     8521 gtcgactcta gaggatcaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     8581 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     8641 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     8701 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     8761 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     8821 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     8881 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg ggg
//
