LOCUS       sequence-174099-        6537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a Myc tagged VSV-G protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-VSV-G-Myc
FEATURES             Location/Qualifiers
     source          1..6537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..48,49..1533)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     CDS             1534..1563
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1793..1812)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1858..1913
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1912..1931)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2271..2287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2271..2287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2284..2306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2295..2311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2319..2325,2326..2343,2344..2349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2364..2385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2502..2519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2673..3261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2753..2772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3432..4223,4224..4292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4055..4074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4293..4397)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4423..4878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4560..4581)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4772..4791
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5005..5027
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5019..5036
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5020..5036
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5046..5065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5046..5064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5185..5564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5565..5768
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5719..5739
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5765..5789
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5880..6452
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgaagtgcc ttttgtactt agccttttta ttcattgggg tgaattgcaa gttcaccata
       61 gtttttccac acaaccaaaa aggaaactgg aaaaatgttc cttctaatta ccattattgc
      121 ccgtcaagct cagatttaaa ttggcataat gacttaatag gcacagcctt acaagtcaaa
      181 atgcccaaga gtcacaaggc tattcaagca gacggttgga tgtgtcatgc ttccaaatgg
      241 gtcactactt gtgatttccg ctggtatgga ccgaagtata taacacattc catccgatcc
      301 ttcactccat ctgtagaaca atgcaaggaa agcattgaac aaacgaaaca aggaacttgg
      361 ctgaatccag gcttccctcc tcaaagttgt ggatatgcaa ctgtgacgga tgccgaagca
      421 gtgattgtcc aggtgactcc tcaccatgtg ctggttgatg aatacacagg agaatgggtt
      481 gattcacagt tcatcaacgg aaaatgcagc aattacatat gccccactgt ccataactct
      541 acaacctggc attctgacta taaggtcaaa gggctatgtg attctaacct catttccatg
      601 gacatcacct tcttctcaga ggacggagag ctatcatccc tgggaaagga gggcacaggg
      661 ttcagaagta actactttgc ttatgaaact ggaggcaagg cctgcaaaat gcaatactgc
      721 aagcattggg gagtcagact cccatcaggt gtctggttcg agatggctga taaggatctc
      781 tttgctgcag ccagattccc tgaatgccca gaagggtcaa gtatctctgc tccatctcag
      841 acctcagtgg atgtaagtct aattcaggac gttgagagga tcttggatta ttccctctgc
      901 caagaaacct ggagcaaaat cagagcgggt cttccaatct ctccagtgga tctcagctat
      961 cttgctccta aaaacccagg aaccggtcct gctttcacca taatcaatgg taccctaaaa
     1021 tactttgaga ccagatacat cagagtcgat attgctgctc caatcctctc aagaatggtc
     1081 ggaatgatca gtggaactac cacagaaagg gaactgtggg atgactgggc accatatgaa
     1141 gacgtggaaa ttggacccaa tggagttctg aggaccagtt caggatataa gtttccttta
     1201 tacatgattg gacatggtat gttggactcc gatcttcatc ttagctcaaa ggctcaggtg
     1261 ttcgaacatc ctcacattca agacgctgct tcgcaacttc ctgatgatga gagtttattt
     1321 tttggtgata ctgggctatc caaaaatcca atcgagcttg tagaaggttg gttcagtagt
     1381 tggaaaagct ctattgcctc ttttttcttt atcatagggt taatcattgg actattcttg
     1441 gttctccgag ttggtatcca tctttgcatt aaattaaagc acaccaagaa aagacagatt
     1501 tatacagaca tagagatgaa ccgacttgga aaggaacaga aactgattag cgaagaagat
     1561 ctgtaactca aatcctgcac aacagattct tcatgtttgg accaaatcaa cttgtgatac
     1621 catgctcaaa gaggcctcaa ttatatttga gtttttaatt tttatgaaaa aaaaaaaaaa
     1681 aaacggaatt cctcgaggga tccgtcgagg aattcactcc tcaggtgcag gctgcctatc
     1741 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     1801 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     1861 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     1921 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     1981 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     2041 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     2101 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     2161 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     2221 ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat gtcatagctg
     2281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2341 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2401 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2461 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2521 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2581 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2641 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3361 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4321 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4381 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     4441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     4501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     4561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     4621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     4681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     4741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     4801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     4861 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4921 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4981 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     5041 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
     5101 gagagcttgg cccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
     5161 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     5221 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5281 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5341 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5401 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     5461 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     5521 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5581 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5641 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5701 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5761 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     5821 tccatagaag acaccgggac cgatccagcc tccggtcgac cgatcctgag aacttcaggg
     5881 tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg
     5941 gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg
     6001 gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc
     6061 ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa
     6121 tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt
     6181 ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta
     6241 taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc
     6301 ttattggtag aaacaactac accctggtca tcatcctgcc tttctcttta tggttacaat
     6361 gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta
     6421 accatgttca tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc
     6481 tgtctcatca ttttggcaaa gaattcctcg acggatccct cgaggaattc tgacact
//
