LOCUS       sequence-174136-       11388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human TRIM5alpha in mammalan cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-humanTRIM5-FOS
FEATURES             Location/Qualifiers
     source          1..11388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1498..1521
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1528..1551
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    1555..1607
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(1609..2183,2184..2186,2187..2195)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1776..1793)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2003..2022
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2196..2873
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(2376..2396)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2594..2613
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2788..2811
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    2909..3497
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2962..2982)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3380..3391)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4016..4196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4222..4239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4228..4452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4498..4926
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4585..4604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4795..4816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4935..4955)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4940..5269
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5120..5255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5317..5364
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5383..5757
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5887..6008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5924..5943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5978..5997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6057..6073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6057..6073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6070..6092)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6081..6097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6105..6111,6112..6129,6130..6135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6150..6171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6288..6305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6459..7047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6539..6558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7218..8009,8010..8078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7841..7860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8079..8183)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8258..8277)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8449..8828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8830..9028
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9046..9226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9273..9398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9895..10128
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10313..10357
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10631..10748
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10849..11152
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11153..11356
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      join(11383..11388,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcttctg gaatcctggt taatgtaaag gaggaggtga cctgccccat ctgcctggaa
       61 ctcctgacac aacccctgag cctggactgc ggccacagct tctgccaagc atgcctcact
      121 gcaaaccaca agaagtccat gctagacaaa ggagagagta gctgccctgt gtgccggatc
      181 agttaccagc ctgagaacat acggcctaat cggcatgtag ccaacatagt ggagaagctc
      241 agggaggtca agttgagccc agaggggcag aaagttgatc attgtgcacg ccatggagag
      301 aaacttctac tcttctgtca ggaggacggg aaggtcattt gctggctttg tgagcggtct
      361 caggagcacc gtggtcacca cacgttcctc acagaggagg ttgcccggga gtaccaagtg
      421 aagctccagg cagctctgga gatgctgagg cagaagcagc aagaggccga ggaattagaa
      481 gctgacatca gagaagagaa agcttcctgg aagactcaaa tacagtatga caaaaccaac
      541 gtcttggcag attttgagca actgagagac atcctggact gggaggagag caatgagctg
      601 caaaacctgg agaaggagga ggaagacatt ctgaaaagcc ttacgaactc tgaaactgag
      661 atggtgcagc agacccagtc cctgagagag ctcatctcag atctggagca tcggctgcag
      721 gggtcagtga tggagctgct tcagggtgtg gatggcgtca taaaaaggac ggagaacgtg
      781 accttgaaga agccagaaac ttttccaaaa aatcaaagga gagtgtttcg agctcctgat
      841 ctgaaaggaa tgctagaagt gtttagagag ctgacagatg tccgacgcta ctgggttgat
      901 gtgacagtgg ctccaaacaa catttcatgt gctgtcattt ctgaagataa gagacaagtg
      961 agctctccga aaccacagat aatatatggg gcacgaggga caagatacca gacatttgtg
     1021 aatttcaatt attgtactgg catcctgggc tctcaaagta tcacatcagg gaaacattac
     1081 tgggaggtag acgtgtccaa gaaaactgct tggatcctgg gggtatgtgc tggcttccaa
     1141 cctgatgcaa tgtgtaatat tgaaaaaaat gaaaattatc aacctaaata cggctactgg
     1201 gttatagggt tagaggaagg agttaaatgt agtgctttcc aggatagttc cttccatact
     1261 ccttctgttc ctttcattgt gcccctctct gtgattattt gtcctgatcg tgttggagtt
     1321 ttcctagact atgaggcttg cactgtctca ttcttcaata tcacaaacca tggatttctc
     1381 atctataagt tttctcactg ttctttttct cagcctgtat ttccatattt aaatcctaga
     1441 aaatgtggag tccccatgac tctgtgctca ccaagctctg gtaccggatc cggattcgat
     1501 tacaaggatg acgatgacaa gagcgcttgg agccacccgc agttcgaaaa ataactcgag
     1561 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccgcc cctctccctc
     1621 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     1681 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     1741 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     1801 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     1861 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     1921 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     1981 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     2041 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     2101 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     2161 cctttgaaaa acacgatgat aatatggcca caaccatgga tagcactgag aacgtcatca
     2221 agcccttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     2281 tcgagggcga gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaggtga
     2341 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
     2401 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg
     2461 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     2521 aggactcctc cctgcaggac ggcaccttca tctaccacgt gaagttcatc ggcgtgaact
     2581 tcccctccga cggccccgta atgcagaaga agactctggg ctgggagccc tccaccgagc
     2641 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggcgctg aagctgaagg
     2701 gcggcggcca ctacctggtg gagttcaagt caatctacat ggccaagaag cccgtgaagc
     2761 tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac gaggactaca
     2821 ccgtggtgga gcagtacgag cgcgccgagg cccgccacca cctgttccag tagtctagag
     2881 ttaacatcga gggatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2941 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3001 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3061 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3121 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3181 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3241 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3301 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3361 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3421 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3481 ttgggccgcc tccccgcatc gataccgtcg agacctagaa aaacatggag caatcacaag
     3541 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     3601 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     3661 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3721 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     3781 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     3841 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     3901 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     3961 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     4021 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4081 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4141 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     4201 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4441 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4501 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4561 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4621 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4681 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4741 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4801 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4861 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4921 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4981 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5041 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5101 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5161 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     5221 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     5281 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     5341 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     5401 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     5461 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     5521 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     5581 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     5641 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     5701 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     5761 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5821 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5881 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5941 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6001 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6061 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6961 tcttgatctg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     8221 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     8281 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     8341 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     8401 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     8461 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     8521 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     8581 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     8641 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     8701 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     8761 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     8821 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     8881 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     8941 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     9001 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     9061 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9121 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9181 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     9241 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     9301 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9361 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9421 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9481 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9541 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9601 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9661 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9721 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9781 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9841 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9901 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9961 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10021 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10081 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10141 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    10201 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10261 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    10321 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    10381 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    10441 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10501 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    10561 tggagagaga gacagagaca gatccattcg attagtgaac ggatctacaa atggcagtat
    10621 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10681 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    10741 aaaattttcg ggtttattac agggacagca gaaattcact ttgaattaat tcgattaata
    10801 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    10861 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    10921 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    10981 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    11041 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    11101 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    11161 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    11221 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    11281 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    11341 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagccacc
//
