LOCUS       sequence-174137-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptavidin that has unimpaired biotin binding after dye labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Jacobsen MT, Fairhead M, Fogelstrand P, Howarth M
  TITLE     Amine Landscaping to Maximize Protein-Dye Fluorescence and 
            Ultrastable Protein-Ligand Interaction.
  JOURNAL   Cell Chem Biol. 2017 Jul 17. pii: S2451-9456(17)30229-5. doi: 
            10.1016/j.chembiol.2017.06.015.
  PUBMED    28757182
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2017 Jul 17. pii: S2451-9456(17)30229-5. doi: 
            10.1016/j.chembiol.2017.06.015."
COMMENT     Sequence Label: pET21-Streptavidin-K121R
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             474..491
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(544..562)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      558..605
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(729..748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             1021..1030
                     /estimated_length=10
     gap             1101..1116
                     /estimated_length=16
     gap             1118..1146
                     /estimated_length=29
ORIGIN
        1 cnnntagaaa tnantttgtt taactttaag aaggagatat acatatggct gaagctggta
       61 tcaccggcac ctggtacaac cagctgggat ccaccttcat cgttaccgct ggtgctgacg
      121 gtgctctgac cggtacctac gaatccgctg ttggtaacgc tgaatctaga tacgttctga
      181 ccggtcgtta cgactccgct ccggctaccg acggttccgg aaccgctctg ggttggaccg
      241 ttgcttggaa aaacaactac cgtaacgctc actccgctac cacctggtct ggccagtacg
      301 ttggtggtgc tgaagctcgt atcaacaccc agtggttgtt gacctccggc accaccgaag
      361 ctaacgcgtg gcgatccacc ctggttggtc acgacacctt caccaaagtt aaaccgtccg
      421 ctgcttccga agaggaagag gaagagtaat aaaagcttgc ggccgcactc gagcaccacc
      481 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      541 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      601 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      781 tcaagctcta aatcgggggc tccctttang gttccgattt agtgctttac ggcacctcga
      841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacngg
      901 tttttcgccc tttgacgttg gagtccacgt tnctttaata gnggnntctt gttccaaact
      961 gnnncnannc tcaaccnatc tcggnctatt cttttgantt atnannnatt ttgnccnatt
     1021 nnnnnnnnnn aaaaaaanga gctgattnnc aaaatttnnn nnnanttnnn nannntnnnn
     1081 ctnnnantnn gnngnnnttt nnnnnnnnnn nnnnnngnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnntnna ncnc
//
