LOCUS       sequence-174146-       12461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12461)
  TITLE     p300
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1245 pCMVb p300
FEATURES             Location/Qualifiers
     source          1..12461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        427..445
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     427..444
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        469..772
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        773..976
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     927..947
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     973..997
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1078..1094
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1078..1094
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4305..6155
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5919..5928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          9062..9634
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(9766..9785)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9831..9886
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9885..9904)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10205..10222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10376..10964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10456..10475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11135..11926,11927..11995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11758..11777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11996..12100)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12168..12186
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12224..12246)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12346..12365
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
       61 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
      121 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
      181 gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc tgaccatttc
      241 cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac cgactctgac
      301 ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta cacaattagg
      361 cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      421 catacgattt aggtgacact atagaataca cggaattcga gctcgccccg ttacataact
      481 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      541 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      601 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      661 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      721 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      781 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      841 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      901 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      961 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1021 ttttgacctc catagaagac accgggaccg atccagcctc cgggggatcg cggccgctct
     1081 agaactagtg gatcccccgg gccgaagaag agatttcctg aggattctgg ttttcctcgc
     1141 ttgtatctcc gaaagaatta aaaatggccg agaatgtggt ggaaccgggg ccgccttcag
     1201 ccaagcggcc taaactctca tctccggccc tctcggcgtc cgccagcgat ggcacagatt
     1261 ttggctctct atttgacttg gagcacgact taccagatga attaatcaac tctacagaat
     1321 tgggactaac caatggtggt gatattaatc agcttcagac aagtcttggc atggtacaag
     1381 atgcagcttc taaacataaa cagctgtcag aattgctgcg atctggtagt tcccctaacc
     1441 tcaatatggg agttggtggc ccaggtcaag tcatggccag ccaggcccaa cagagcagtc
     1501 ctggattagg tttgataaat agcatggtca aaagcccaat gacacaggca ggcttgactt
     1561 ctcccaacat ggggatgggc actagtggac caaatcaggg tcctacgcag tcaacaggta
     1621 tgatgaacag tccagtaaat cagcctgcca tgggaatgaa cacagggatg aatgcgggca
     1681 tgaatcctgg aatgttggct gcaggcaatg gacaagggat aatgcctaat caagtcatga
     1741 acggttcaat tggagcaggc cgagggcgac agaatatgca gtacccaaac ccaggcatgg
     1801 gaagtgctgg caacttactg actgagcctc ttcagcaggg ctctccccag atgggaggac
     1861 aaacaggatt gagaggcccc cagcctctta agatgggaat gatgaacaac cccaatcctt
     1921 atggttcacc atatactcag aatcctggac agcagattgg agccagtggc cttggtctcc
     1981 agattcagac aaaaactgta ctatcaaata acttatctcc atttgctatg gacaaaaagg
     2041 cagttcctgg tggaggaatg cccaacatgg gtcaacagcc agccccgcag gtccagcagc
     2101 caggtctggt gactccagtt gcccaaggga tgggttctgg agcacataca gctgatccag
     2161 agaagcgcaa gctcatccag cagcagcttg ttctcctttt gcatgctcac aagtgccagc
     2221 gccgggaaca ggccaatggg gaagtgaggc agtgcaacct tccccactgt cgcacaatga
     2281 agaatgtcct aaaccacatg acacactgcc agtcaggcaa gtcttgccaa gtggcacact
     2341 gtgcatcttc tcgacaaatc atttcacact ggaagaattg tacaagacat gattgtcctg
     2401 tgtgtctccc cctcaaaaat gctggtgata agagaaatca acagccaatt ttgactggag
     2461 cacccgttgg acttggaaat cctagctctc taggggtggg tcaacagtct gcccccaacc
     2521 taagcactgt tagtcagatt gatcccagct ccatagaaag agcctatgca gctcttggac
     2581 taccctatca agtaaatcag atgccgacac aaccccaggt gcaagcaaag aaccagcaga
     2641 atcagcagcc tgggcagtct ccccaaggca tgcggcccat gagcaacatg agtgctagtc
     2701 ctatgggagt aaatggaggt gtaggagttc aaacgccgag tcttctttct gactcaatgt
     2761 tgcattcagc cataaattct caaaacccaa tgatgagtga aaatgccagt gtgccctccc
     2821 tgggtcctat gccaacagca gctcaaccat ccactactgg aattcggaaa cagtggcacg
     2881 aagatattac tcaggatctt cgaaatcatc ttgttcacaa actcgtccaa gccatatttc
     2941 ctacgccgga tcctgctgct ttaaaagaca gacggatgga aaacctagtt gcatatgctc
     3001 ggaaagttga aggggacatg tatgaatctg caaacaatcg agcggaatac taccaccttc
     3061 tagctgagaa aatctataag atccagaaag aactagaaga aaaacgaagg accagactac
     3121 agaagcagaa catgctacca aatgctgcag gcatggttcc agtttccatg aatccagggc
     3181 ctaacatggg acagccgcaa ccaggaatga cttctaatgg ccctctacct gacccaagta
     3241 tgatccgtgg cagtgtgcca aaccagatga tgcctcgaat aactccacaa tctggtttga
     3301 atcaatttgg ccagatgagc atggcccagc cccctattgt accccggcaa acccctcctc
     3361 ttcagcacca tggacagttg gctcaacctg gagctctcaa cccgcctatg ggctatgggc
     3421 ctcgtatgca acagccttcc aaccagggcc agttccttcc tcagactcag ttcccatcac
     3481 agggaatgaa tgtaacaaat atccctttgg ctccgtccag cggtcaagct ccagtgtctc
     3541 aagcacaaat gtctagttct tcctgcccgg tgaactctcc tataatgcct ccagggtctc
     3601 aggggagcca cattcactgt ccccagcttc ctcaaccagc tcttcatcag aattcaccct
     3661 cgcctgtacc tagtcgtacc cccacccctc accatactcc cccaagcata ggggctcagc
     3721 agccaccagc aacaacaatt ccagcccctg ttcctacacc tcctgccatg ccacctgggc
     3781 cacagtccca ggctctacat ccccctccaa ggcagacacc tacaccacca acaacacaac
     3841 ttccccaaca agtgcagcct tcacttcctg ctgcaccttc tgctgaccag ccccagcagc
     3901 agcctcgctc acagcagagc acagcagcgt ctgttcctac cccaacagca ccgctgcttc
     3961 ctccgcagcc tgcaactcca ctttcccagc cagctgtaag cattgaagga caggtatcaa
     4021 atcctccatc tactagtagc acagaagtga attctcaggc cattgctgag aagcagcctt
     4081 cccaggaagt gaagatggag gccaaaatgg aagtggatca accagaacca gcagatactc
     4141 agccggagga tatttcagag tctaaagtgg aagactgtaa aatggaatct accgaaacag
     4201 aagagagaag cactgagtta aaaactgaaa taaaagagga ggaagaccag ccaagtactt
     4261 cagctaccca gtcatctccg gctccaggac agtcaaagaa aaagattttc aaaccagaag
     4321 aactacgaca ggcactgatg ccaacattgg aggcacttta ccgtcaggat ccagaatccc
     4381 ttccctttcg tcaacctgtg gaccctcagc ttttaggaat ccctgattac tttgatattg
     4441 tgaagagccc catggatctt tctaccatta agaggaagtt agacactgga cagtatcagg
     4501 agccctggca gtatgtcgat gatatttggc ttatgttcaa taatgcctgg ttatataacc
     4561 ggaaaacatc acgggtatac aaatactgct ccaagctctc tgaggtcttt gaacaagaaa
     4621 ttgacccagt gatgcaaagc cttggatact gttgtggcag aaagttggag ttctctccac
     4681 agacactgtg ttgctacggc aaacagttgt gcacaatacc tcgtgatgcc acttattaca
     4741 gttaccagaa caggtatcat ttctgtgaga agtgtttcaa tgagatccaa ggggagagcg
     4801 tttctttggg ggatgaccct tcccagcctc aaactacaat aaataaagaa caattttcca
     4861 agagaaaaaa tgacacactg gatcctgaac tgtttgttga atgtacagag tgcggaagaa
     4921 agatgcatca gatctgtgtc cttcaccatg agatcatctg gcctgctgga ttcgtctgtg
     4981 atggctgttt aaagaaaagt gcacgaacta ggaaagaaaa taagttttct gctaaaaggt
     5041 tgccatctac cagacttggc acctttctag agaatcgtgt gaatgacttt ctgaggcgac
     5101 agaatcaccc tgagtcagga gaggtcactg ttagagtagt tcatgcttct gacaaaaccg
     5161 tggaagtaaa accaggcatg aaagcaaggt ttgtggacag tggagagatg gcagaatcct
     5221 ttccataccg aaccaaagcc ctctttgcct ttgaagaaat tgatggtgtt gacctgtgct
     5281 tctttggcat gcatgttcaa gagtatggct ctgactgccc tccacccaac cagaggagag
     5341 tatacatatc ttacctcgat agtgttcatt tcttccgtcc taaatgcttg aggactgcag
     5401 tctatcatga aatcctaatt ggatatttag aatatgtcaa gaaattaggt tacacaacag
     5461 ggcatatttg ggcatgtcca ccaagtgagg gagatgatta tatcttccat tgccatcctc
     5521 ctgaccagaa gatacccaag cccaagcgac tgcaggaatg gtacaaaaaa atgcttgaca
     5581 aggctgtatc agagcgtatt gtccatgact acaaggatat ttttaaacaa gctactgaag
     5641 atagattaac aagtgcaaag gaattgcctt atttcgaggg tgatttctgg cccaatgttc
     5701 tggaagaaag cattaaggaa ctggaacagg aggaagaaga gagaaaacga gaggaaaaca
     5761 ccagcaatga aagcacagat gtgaccaagg gagacagcaa aaatgctaaa aagaagaata
     5821 ataagaaaac cagcaaaaat aagagcagcc tgagtagggg caacaagaag aaacccggga
     5881 tgcccaatgt atctaacgac ctctcacaga aactatatgc caccatggag aagcataaag
     5941 aggtcttctt tgtgatccgc ctcattgctg gccctgctgc caactccctg cctcccattg
     6001 ttgatcctga tcctctcatc ccctgcgatc tgatggatgg tcgggatgcg tttctcacgc
     6061 tggcaaggga caagcacctg gagttctctt cactccgaag agcccagtgg tccaccatgt
     6121 gcatgctggt ggagctgcac acgcagagcc aggaccgctt tgtctacacc tgcaatgaat
     6181 gcaagcacca tgtggagaca cgctggcact gtactgtctg tgaggattat gacttgtgta
     6241 tcacctgcta taacactaaa aaccatgacc acaaaatgga gaaactaggc cttggcttag
     6301 atgatgagag caacaaccag caggctgcag ccacccagag cccaggcgat tctcgccgcc
     6361 tgagtatcca gcgctgcatc cagtctctgg tccatgcttg ccagtgtcgg aatgccaatt
     6421 gctcactgcc atcctgccag aagatgaagc gggttgtgca gcataccaag ggttgcaaac
     6481 ggaaaaccaa tggcgggtgc cccatctgca agcagctcat tgccctctgc tgctaccatg
     6541 ccaagcactg ccaggagaac aaatgcccgg tgccgttctg cctaaacatc aagcagaagc
     6601 tccggcagca acagctgcag caccgactac agcaggccca aatgcttcgc aggaggatgg
     6661 ccagcatgca gcggactggt gtggttgggc agcaacaggg cctcccttcc cccactcctg
     6721 ccactccaac gacaccaact ggccaacagc caaccacccc gcagacgccc cagcccactt
     6781 ctcagcctca gcctacccct cccaatagca tgccacccta cttgcccagg actcaagctg
     6841 ctggccctgt gtcccagggt aaggcagcag gccaggtgac ccctccaacc cctcctcaga
     6901 ctgctcagcc accccttcca gggcccccac ctgcagcagt ggaaatggca atgcagattc
     6961 agagagcagc ggagacgcag cgccagatgg cccacgtgca aatttttcaa aggccaatcc
     7021 aacaccagat gcccccgatg actcccatgg cccccatggg tatgaaccca cctcccatga
     7081 ccagaggtcc cagtgggcat ttggagccag ggatgggacc gacagggatg cagcaacagc
     7141 caccctggag ccaaggagga ttgcctcagc cccagcaact acagtctggg atgccaaggc
     7201 cagccatgat gtcagtggcc cagcatggtc aacctttgaa catggctcca caaccaggat
     7261 tgggccaggt aggtatcagc ccactcaaac caggcactgt gtctcaacaa gccttacaaa
     7321 accttttgcg gactctcagg tctcccagct ctcccctgca gcagcaacag gtgcttagta
     7381 tccttcacgc caacccccag ctgttggctg cattcatcaa gcagcgggct gccaagtatg
     7441 ccaactctaa tccacaaccc atccctgggc agcctggcat gccccagggg cagccagggc
     7501 tacagccacc taccatgcca ggtcagcagg gggtccactc caatccagcc atgcagaaca
     7561 tgaatccaat gcaggcgggc gttcagaggg ctggcctgcc ccagcagcaa ccacagcagc
     7621 aactccagcc acccatggga gggatgagcc cccaggctca gcagatgaac atgaaccaca
     7681 acaccatgcc ttcacaattc cgagacatct tgagacgaca gcaaatgatg caacagcagc
     7741 agcaacaggg agcagggcca ggaataggcc ctggaatggc caaccataac cagttccagc
     7801 aaccccaagg agttggctac ccaccacagc agcagcagcg gatgcagcat cacatgcaac
     7861 agatgcaaca aggaaatatg ggacagatag gccagcttcc ccaggccttg ggagcagagg
     7921 caggtgccag tctacaggcc tatcagcagc gactccttca gcaacagatg gggtcccctg
     7981 ttcagcccaa ccccatgagc ccccagcagc atatgctccc aaatcaggcc cagtccccac
     8041 acctacaagg ccagcagatc cctaattctc tctccaatca agtgcgctct ccccagcctg
     8101 tcccttctcc acggccacag tcccagcccc cccactccag tccttcccca aggatgcagc
     8161 ctcagccttc tccacaccac gtttccccac agacaagttc cccacatcct ggactggtag
     8221 ctgcccaggc caaccccatg gaacaagggc attttgccag cccggaccag aattcaatgc
     8281 tttctcagct tgctagcaat ccaggcatgg caaacctcca tggtgcaagc gccacggacc
     8341 tgggactcag caccgataac tcagacttga attcaaacct ctcacagagt acactagaca
     8401 tacactagag acaccttgta gtattttggg agcaaaaaaa ttattttctc ttaacaagac
     8461 tttttgtact gaaaacaatt tttttgaatc tttcgtagcc taaaagacaa ttttccttgg
     8521 aacacataag aactgtgcag tagccgtttg tggtttaaag caaacatgca agatgaacct
     8581 gagggatgat agaatacaaa gaatatattt ttgttatggc tggttaccac cagcctttct
     8641 tcccctttgt gtgtgtggtt caagtgtgca ctgggaggag gctgaggcct gtgaagccaa
     8701 acaatatgct cctgccttgc acctccaata ggttttatta ttttttttaa attaatgaac
     8761 atatgtaata ttaatagtta ttatttactg gtgcagatgg ttgacatttt tccctatttt
     8821 cctcacttta tggaagagtt aaaacatttc taaaccagag gacaaaaggg gttaatgtta
     8881 ctttaaaatt acattctata tatatataaa tatatataaa tatatattaa aataccagtt
     8941 ttttttctct gggtgcaaag atgttcattc ttttaaaaaa tgtttaaaaa aaaggaattc
     9001 gatatcaagc ttatgcatag atctatgcat agatctatgc atagatcctg agaacttcag
     9061 ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
     9121 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
     9181 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
     9241 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
     9301 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     9361 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     9421 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     9481 tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca
     9541 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     9601 taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt
     9661 gctgtctcat cattttggca aagaattcac tcctcaggtg caggctgcct atcagaaggt
     9721 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     9781 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     9841 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     9901 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     9961 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
    10021 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
    10081 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgc
    10141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12121 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    12181 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    12241 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    12301 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    12361 ttgtactgag agtgcaccat tcgacgctct cccttatgcg actcctgcat taggaagcag
    12421 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa g
//
