LOCUS       sequence-174151-        6650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Boehm JS, Hession MT, Bulmer SE, Hahn WC
  TITLE     Transformation of human and murine fibroblasts without viral 
            oncoproteins.
  JOURNAL   Mol Cell Biol. 2005 Aug . 25(15):6464-74.
  PUBMED    16024784
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Aug . 25(15):6464-74."
COMMENT     Sequence Label: pWZL Blast myc
FEATURES             Location/Qualifiers
     source          1..6650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             19..488
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    553..752
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             953..1369
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1299..1321
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1337..1353
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1420..2739
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    3078..3539
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3134..3151)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3361..3380
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4155..4624
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4754..4771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4925..5513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5005..5024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5673..6464,6465..6533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6296..6315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6534..6638)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct
       61 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
      121 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
      181 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
      241 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
      301 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
      361 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
      421 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
      481 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
      541 ccaccgggag gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
      601 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
      661 ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga
      721 ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc
      781 gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga
      841 atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat
      901 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
      961 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1021 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1081 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1141 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1201 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1261 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1321 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccc
     1381 agtgtggtgg tacagcttgg gctgcaggtc gactctagta tgcccctcaa cgttagcttc
     1441 accaacagga actatgacct cgactacgac tcggtgcagc cgtatttcta ctgcgacgag
     1501 gaggagaact tctaccagca gcagcagcag agcgagctgc agcccccggc gcccagcgag
     1561 gatatctgga agaaattcga gctgctgccc accccgcccc tgtcccctag ccgccgctcc
     1621 gggctctgct cgccctccta cgttgcggtc acacccttct cccttcgggg agacaacgac
     1681 ggcggtggcg ggagcttctc cacggccgac cagctggaga tggtgaccga gctgctggga
     1741 ggagacatgg tgaaccagag tttcatctgc gacccggacg acgagacctt catcaaaaac
     1801 atcatcatcc aggactgtat gtggagcggc ttctcggccg ccgccaagct cgtctcagag
     1861 aagctggcct cctaccaggc tgcgcgcaaa gacagcggca gcccgaaccc cgcccgcggc
     1921 cacagcgtct gctccacctc cagcttgtac ctgcaggatc tgagcgccgc cgcctcagag
     1981 tgcatcgacc cctcggtggt cttcccctac cctctcaacg acagcagctc gcccaagtcc
     2041 tgcgcctcgc aagactctag cgccttctct ccgtcctcgg attctctgct ctcctcgacg
     2101 gagtcctccc cgcagggcag ccccgagccc ctggtgctcc atgaggagac accgcccacc
     2161 accagcagcg actctgagga ggaacaagaa gatgaggaag aaatcgatgt tgtttctgtg
     2221 gaaaagaggc aggctcctgg caaaaggtca gagtctggat caccttctgc tggaggccac
     2281 agcaaacctc ctcacagccc actggtcctc aagaggtgcc acgtctccac acatcagcac
     2341 aactacgcag cgcctccctc cactcggaag gactatcctg ctgccaagag ggtcaagttg
     2401 gacagtgtca gagtcctgag acagatcagc aacaaccgaa aatgcaccag ccccaggtcc
     2461 tcggacaccg aggagaatgt caagaggcga acacacaacg tcttggagcg ccagaggagg
     2521 aacgagctaa aacggagctt ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat
     2581 gaaaaggccc ccaaggtagt tatccttaaa aaagccacag catacatcct gtccgtccaa
     2641 gcagaggagc aaaagctcat ttctgaagag gacttgttgc ggaaacgacg agaacagttg
     2701 aaacacaaac ttgaacagct acggaactct tgtgcgtaag gaaaagtaag gaaaatgatt
     2761 ccttctaaca gaaatgtcct gagcaatcac ctatgaactt gtttcaaatg catgatcaaa
     2821 tgcaacctca caaccttggc tgagtcttga gactgaaaga tttagcatct tttttttttc
     2881 tttaacagat ttgtatttaa gaattgtttt taaaaaattt taagatttac acaatgtttc
     2941 tctgtaaata ttgccattaa atgtaaataa cttccgaatt gggcgagctc gaattcactc
     3001 gagatacgta aaacaattga agatctagtc gaccaattcc ggttattttc caccatattg
     3061 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3121 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3181 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3241 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3301 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3361 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3421 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3481 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     3541 accatgaaaa catttaacat ttctcaacag gatctagaat tagtagaagt agcgacagag
     3601 aagattacaa tgctttatga ggataataaa catcatgtgg gagcggcaat tcgtacgaaa
     3661 acaggagaaa tcatttcggc agtacatatt gaagcgtata taggacgagt aactgtttgt
     3721 gcagaagcca ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt tgacacgatt
     3781 gtagctgtta gacaccctta ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct
     3841 tgtggtatgt gtagggagtt gatttcagac tatgcaccag attgttttgt gttaatagaa
     3901 atgaatggca agttagtcaa aactacgatt gaagaactca ttccactcaa atatacccga
     3961 aattaaaagt tttaccatac caagctcatc gataaaataa aagattttat ttagtctcca
     4021 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     4081 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     4141 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4201 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     4261 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     4321 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     4381 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     4441 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     4501 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     4561 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     4621 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     4681 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc
     5641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
//
