LOCUS       sequence-174153-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against TOM40..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Yano H, Baranov SV, Baranova OV, Kim J, Pan Y, Yablonska S, Carlisle
            DL, Ferrante RJ, Kim AH, Friedlander RM
  TITLE     Inhibition of mitochondrial protein import by mutant huntingtin.
  JOURNAL   Nat Neurosci. 2014 Jun;17(6):822-31. doi: 10.1038/nn.3721. Epub 2014
            May 18.
  PUBMED    24836077
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3721"; 
            journalName: "Nat Neurosci"; date: "2014-06"; volume: "17"; issue: 
            "6"; pages: "822-31"
COMMENT     Sequence Label: pBSU6-shTom40.3/CMV-eGFP
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..33
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17..33
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        30..343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     262..282
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        491..794
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     949..969
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggcgattgnn nnnngctcta gaactagtgg atccgacgcc gccatctcta ggcccgcgcc
       61 ggccccctcg cacagacttg tgggagaagc tcggctactc ccctgccccg gttaatttgc
      121 atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag ataatctgtt
      181 ctttttaata ctagctacat tttacatgat aggcttggat ttctataaga gatacaaata
      241 ctaaattatt attttaaaaa acagcacaaa aggaaactca ccctaactgt aaagtaattg
      301 tgtgttttga gactataaat atcccttgga gaaaagcctt gtttggcact gtcatgtctc
      361 tagcattcaa gcttagctag agacatgaca gtgccttttt gaattcctgc agggtattac
      421 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      481 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      541 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      601 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      661 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      721 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      781 tcgctattac catggtgatg cggntttggc agtacatcaa tgggcgtgga tagcggtttg
      841 actcacgggg anttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      901 naaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      961 gtaggcgtgt anggngggag gnntannna
//
