LOCUS       sequence-174158-       11105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER-targeted mCherry-tagged recruiter construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: pHR-TcRb:mCherry-FKBP
FEATURES             Location/Qualifiers
     source          1..11105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      27..162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1310..1375
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1505..1525
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1950..2084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1987..2006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2041..2060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2252..2885
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2932..3057
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3554..3787
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3972..4016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4301..4418
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4571..4978
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      5060..5069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5132..5176
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6077..6784
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6222..6240)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6490..6509
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      6794..6803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6806..6832
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6833..6835,6836..7159)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    7220..7808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7273..7293)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7691..7702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7811..7827)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7812..7828)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7918..8151
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8176..8194)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8177..8194)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8484..8503)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8717..8736)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8836..8858
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8896..8914)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8982..9086
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9087..9155,9156..9947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9305..9324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10118..10706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10607..10626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10860..10877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10947..10967)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10952..11105,1..176)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
       61 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
      121 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      181 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
      241 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
      301 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
      361 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
      421 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
      481 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
      541 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
      601 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
      661 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
      721 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
      781 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
      841 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
      901 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
      961 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     1021 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     1081 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     1141 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     1201 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     1261 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     1321 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     1381 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     1441 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     1501 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     1561 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     1621 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     1681 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     1741 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     1801 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     1861 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1921 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     2101 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     2161 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     2221 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     2281 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     2341 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     2401 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     2461 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     2521 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     2581 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     2641 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     2701 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2761 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2821 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2881 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     2941 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     3001 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     3061 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     3121 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     3181 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     3241 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     3301 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     3361 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     3421 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     3481 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     3541 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     3601 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     3661 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3721 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3781 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3841 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3901 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     3961 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4021 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4081 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     4141 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     4201 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4261 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4321 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4381 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4441 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     4501 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     4561 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     4621 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     4681 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     4741 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     4801 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     4861 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     4921 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     4981 ggatctggag ctctcgagaa ttctcacgcg tctgcaggat atcaagcttg cggtaccgcg
     5041 ggcccgggat ccaccggtcg ccaccatgga ctcctggacc ttctgctgtg tgtccctttg
     5101 catcctggta gcgaagcata cagatgctgg aggcctgaac gacatcttcg aggctcagaa
     5161 aatcgaatgg cacgaaggca gcgttatcca gtcaccccgc catgaggtga cagagatggg
     5221 acaagaagtg actctgagat gtaaaccaat ttcaggccac aactcccttt tctggtacag
     5281 acagaccatg atgcggggac tggagttgct catttacttt aacaacaacg ttccgataga
     5341 tgattcaggg atgcccgagg atcgattctc agctaagatg cctaatgcat cattctccac
     5401 tctgaagatc cagccctcag aacccaggga ctcagctgtg tacttctgtg ccagcagttt
     5461 ctcgacctgt tcggctaact atggctacac cttcggttcg gggaccaggt taaccgttgt
     5521 agaggacctg aacaaggtgt tcccacccga ggtcgctgtg tttgagccat cagaagcaga
     5581 aatctcccac acccaaaagg ccacactggt gtgcctggcc acaggcttct tccccgacca
     5641 cgtggagctg agctggtggg tgaatgggaa ggaggtgcac agtggggtca gcacagaccc
     5701 gcagcccctc aaggagcagc ccgccctcaa tgactccaga tactgcctga gcagccgcct
     5761 gagggtctcg gccaccttct ggcagaaccc ccgcaaccac ttccgctgtc aagtccagtt
     5821 ctacgggctc tcggagaatg acgagtggac ccaggatagg gccaaacccg tcacccagat
     5881 cgtcagcgcc gaggcctggg gtagagcaga ctgtggcttt acctcggtgt cctaccagca
     5941 aggggtcctg tctgccacca tcctctatga gatcctgcta gggaaggcca ccctgtatgc
     6001 tgtgctggtc agcgcccttg tgttgatggc catggtcaag agaaaggatt tcggtgctgc
     6061 acttctgccc ggttctatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     6121 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     6181 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     6241 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     6301 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     6361 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     6421 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     6481 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     6541 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     6601 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     6661 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     6721 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     6781 caagcccggt tctgccgcca tggcatatcc atatgacgtc ccagactatg ctatgggagt
     6841 gcaggtggag actatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
     6901 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag
     6961 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
     7021 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
     7081 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
     7141 gcttctaaaa ctggaataaa gcggccgcga ctctagagtc gacctgcagg catgcaagct
     7201 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7261 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7321 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7381 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7441 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7501 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7561 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7621 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7681 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7741 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7801 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     7861 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7921 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7981 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8041 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8101 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     8161 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     8221 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     8281 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     8341 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     8401 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     8461 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     8521 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     8581 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     8641 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     8701 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8761 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8821 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8881 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8941 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9001 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9061 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9121 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9181 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9241 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9301 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9361 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9421 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9481 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9541 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9601 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9661 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9721 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9781 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9841 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9901 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9961 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10021 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10081 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10141 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10201 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10261 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10321 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10381 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10441 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10501 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10561 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10621 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10681 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10741 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10801 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10861 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10921 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    10981 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    11041 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    11101 agcaa
//
