LOCUS       sequence-174172-       10732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recycling endosome-targeted mCherry-tagged recruiter construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: pHR-FKBP:mCherry-Rab11a
FEATURES             Location/Qualifiers
     source          1..10732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1311..1376
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1506..1526
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1951..2085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1988..2007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2042..2061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2253..2886
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2933..3058
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3555..3788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3973..4017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4302..4419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4572..4979
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      5013..5022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5019..5021,5022..5342)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             5367..6074
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5512..5530)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5780..5799
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6087..6113
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6114..6140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6848..7436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6901..6921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7319..7330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7439..7455)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7440..7456)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7546..7779
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7804..7822)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7805..7822)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8112..8131)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8345..8364)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8464..8486
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8524..8542)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8610..8714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8715..8783,8784..9575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8933..8952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9746..10334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10235..10254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10488..10505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10575..10595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10580..10732,1..177)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
       61 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      181 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
      241 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
      301 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
      361 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
      421 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
      481 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
      541 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
      601 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
      661 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
      721 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
      781 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
      841 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
      901 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
      961 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     1021 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     1081 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
     1141 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
     1201 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
     1261 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     1321 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     1381 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     1441 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     1501 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     1561 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     1621 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     1681 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     1741 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     1801 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     1861 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     1921 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     1981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     2101 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     2161 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     2221 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     2281 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     2341 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     2401 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     2461 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     2521 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     2581 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     2641 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     2701 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2881 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     2941 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     3001 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     3061 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     3121 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     3181 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     3241 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     3301 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     3361 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     3421 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3481 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3541 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3601 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3661 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3721 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3781 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     3841 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     3901 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     3961 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     4021 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     4081 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4141 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     4201 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     4261 ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg
     4321 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4381 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     4441 agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa
     4501 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     4561 agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga
     4621 agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc
     4681 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
     4741 cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa
     4801 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
     4861 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
     4921 aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccgggg
     4981 gggatctgga gctctcgaga attctcacgc gtgccaccat gggagtgcag gtggagacta
     5041 tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca
     5101 ccgggatgct tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta
     5161 agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga
     5221 gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc
     5281 acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg
     5341 aaggtgctgc acttctgccc ggttctatgg tgagcaaggg cgaggaggat aacatggcca
     5401 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     5461 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     5521 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     5581 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     5641 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     5701 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     5761 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     5821 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     5881 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     5941 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     6001 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     6061 acgagctgta caagcccggt tctatgtacc catacgatgt tccggattac gcttacccat
     6121 acgatgttcc ggattacgct ggtacccgcg acgacgagta cgactacctc tttaaagttg
     6181 tccttattgg agattctggt gttggaaaga gtaatctcct gtctcgattt actcgaaatg
     6241 agtttaatct ggaaagcaag agcaccattg gagtagagtt tgcaacaaga agcatccagg
     6301 ttgatggaaa aacaataaag gcacagatat gggacacagc agggcaagag cgatatcgag
     6361 ctataacatc agcatattat cgtggagctg taggtgcctt attggtttat gacattgcta
     6421 aacatctcac atatgaaaat gtagagcgat ggctgaaaga actgagagat catgctgata
     6481 gtaacattgt tatcatgctt gtgggcaata agagtgatct acgtcatctc agggcagttc
     6541 ctacagatga agcaagagct tttgcagaaa agaatggttt gtcattcatt gaaacttcgg
     6601 ccctagactc tacaaatgta gaagctgctt ttcagacaat tttaacagag atttaccgca
     6661 ttgtttctca gaagcaaatg tcagacagac gcgaaaatga catgtctcca agcaacaatg
     6721 tggttcctat tcatgttcca ccaaccactg aaaacaagcc aaaggtgcag tgctgtcaga
     6781 acatctaagc ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     6841 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6901 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6961 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7021 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7081 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7141 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7201 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7261 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7321 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7381 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7441 ataccgtcga cctcgaggga attaattcga gctcggtacc tttaagacca atgacttaca
     7501 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7561 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7621 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7681 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7741 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     7801 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     7861 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     7921 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     7981 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     8041 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     8101 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     8161 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     8221 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     8281 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     8341 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     8401 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     8461 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     8521 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8581 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8641 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8701 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8761 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8821 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8881 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8941 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9001 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9061 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9121 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9181 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9241 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9301 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9361 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9421 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9481 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9541 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9601 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9661 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9721 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9781 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9841 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9901 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9961 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10021 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10081 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10141 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10201 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10261 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10321 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10381 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10441 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10501 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10561 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10621 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10681 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag ca
//
