LOCUS       sequence-174173-       10708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recruitable Akt2 (LocaTOR2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: pHR-FRB:Akt2
FEATURES             Location/Qualifiers
     source          1..10708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1206..1271
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1401..1421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1846..1980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1883..1902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1937..1956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2148..2781
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2828..2953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3450..3683
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3868..3912
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4197..4314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4467..4874
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4917..5195
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     misc_feature    6719..7307
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6772..6792)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7190..7201)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7310..7326)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7311..7327)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7417..7650
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7675..7693)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7676..7693)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7983..8002)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8216..8235)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8335..8357
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8395..8413)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8481..8585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8586..8654,8655..9446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8804..8823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9617..10205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10106..10125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10359..10376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10446..10466)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10451..10708,1..72)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10631..10708,1..58)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
       61 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
      121 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
      181 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
      241 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
      301 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
      361 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
      421 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
      481 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
      541 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
      601 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
      661 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
      721 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
      781 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
      841 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
      901 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
      961 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     1021 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     1081 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     1141 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     1201 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     1261 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     1321 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     1381 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     1441 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     1501 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     1561 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     1621 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     1681 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     1741 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1801 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     1981 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     2041 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     2101 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     2161 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2221 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     2281 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     2341 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     2401 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     2461 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     2521 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     2581 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     2641 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2701 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2761 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2821 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2881 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2941 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     3001 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     3061 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     3121 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3181 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3241 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     3301 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     3361 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     3421 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     3481 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     3541 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     3601 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     3661 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     3721 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     3781 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     3841 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     3901 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     3961 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     4021 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     4081 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     4141 attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat ccacaatttt
     4201 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4261 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     4321 tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga attcctgcag
     4381 ccccgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     4441 cctgtaggtt tggcaagcta gctgcagtaa cgccattttg caaggcatgg aaaaatacca
     4501 aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc
     4561 aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca
     4621 ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc
     4681 tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc
     4741 ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc
     4801 ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgacaga
     4861 ctgagtcgcc cgggggggat ctggagctct cgagaattct cacgcgcgcc accatgatcc
     4921 tctggcatga gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa
     4981 ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca tgctatgatg gaacggggcc
     5041 cccagactct gaaggaaaca tcctttaatc aggcctatgg tcgagattta atggaggccc
     5101 aagagtggtg caggaagtac atgaaatcag ggaatgtcaa ggacctcctc caagcctggg
     5161 acctctatta tcatgtgttc cgacgaatct caaagacgcg tgccaccatg aatgaggtgt
     5221 ctgtcatcaa agaaggctgg ctccacaagc gtggtgaata catcaagacc tggaggccac
     5281 ggtacttcct gctgaagagc gacggctcct tcattgggta caaggagagg cccgaggccc
     5341 ctgatcagac tctacccccc ttaaacaact tctccgtagc agaatgccag ctgatgaaga
     5401 ccgagaggcc gcgacccaac acctttgtca tacgctgcct gcagtggacc acagtcatcg
     5461 agaggacctt ccacgtggat tctccagacg agagggagga gtggatgcgg gccatccaga
     5521 tggtcgccaa cagcctcaag cagcgggccc caggcgagga ccccatggac tacaagtgtg
     5581 gctcccccag tgactcctcc acgactgagg agatggaagt ggcggtcagc aaggcacggg
     5641 ctaaagtgac catgaatgac ttcgactatc tcaaactcct tggcaaggga acctttggca
     5701 aagtcatcct ggtgcgggag aaggccactg gccgctacta cgccatgaag atcctgcgga
     5761 aggaagtcat cattgccaag gatgaagtcg ctcacacagt caccgagagc cgggtcctcc
     5821 agaacaccag gcacccgttc ctcactgcgc tgaagtatgc cttccagacc cacgaccgcc
     5881 tgtgctttgt gatggagtat gccaacgggg gtgagctgtt cttccacctg tcccgggagc
     5941 gtgtcttcac agaggagcgg gcccggtttt atggtgcaga gattgtctcg gctcttgagt
     6001 acttgcactc gcgggacgtg gtataccgcg acatcaagct ggaaaacctc atgctggaca
     6061 aagatggcca catcaagatc actgactttg gcctctgcaa agagggcatc agtgacgggg
     6121 ccaccatgaa aaccttctgt gggaccccgg agtacctggc gcctgaggtg ctggaggaca
     6181 atgactatgg ccgggccgtg gactggtggg ggctgggtgt ggtcatgtac gagatgatgt
     6241 gcggccgcct gcccttctac aaccaggacc acgagcgcct cttcgagctc atcctcatgg
     6301 aagagatccg cttcccgcgc acgctcagcc ccgaggccaa gtccctgctt gctgggctgc
     6361 ttaagaagga ccccaagcag aggcttggtg gggggcccag cgatgccaag gaggtcatgg
     6421 agcacaggtt cttcctcagc atcaactggc aggacgtggt ccagaagaag ctcctgccac
     6481 ccttcaaacc tcaggtcacg tccgaggtcg acacaaggta cttcgatgat gaatttaccg
     6541 cccagtccat cacaatcaca ccccctgacc gctatgacag cctgggctta ctggagctgg
     6601 accagcggac ccacttcccc cagttctcct actcggccag catccgcgag gatccatgag
     6661 cggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa
     6721 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6781 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6841 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6901 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6961 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7021 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7081 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7141 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7201 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7261 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7321 acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     7381 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7441 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7501 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7561 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7621 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     7681 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     7741 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     7801 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     7861 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     7921 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     7981 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     8041 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     8101 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     8161 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     8221 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8281 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     8341 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     8401 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     8461 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8521 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8581 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8641 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8701 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8761 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8821 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8881 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8941 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9001 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9061 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9121 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9181 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9241 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9301 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9361 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9421 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9481 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9541 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9661 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9721 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9781 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9841 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9901 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9961 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10021 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10081 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10141 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10201 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10261 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10321 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10381 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10441 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    10501 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    10561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    10621 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10681 actaattttt tttatttatg cagaggcc
//
